Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,025,532 G→A 8.0% V368V (GTC→GTT)  PP_2641 ← iron‑sulfur cluster‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,025,5320GA 8.0% 142.5 / 2.7 50V368V (GTC→GTT) PP_2641iron‑sulfur cluster‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCGCCCATCGCCTGAAGGGTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCTCGCGGGCCTGGCTGATCATCGTGGCA  >  NC_002947/3025386‑3025679
                                                                                                                                                  |                                                                                                                                                   
gcgcCCATCGCCTTAAGGGTATGCGCGGCCATCTCCAGGGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTTGGCCGAc                                                                                                                                                   <  7:343618/149‑1 (MQ=255)
      aTCGCCTGAAGGGTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTc                                                                                                                                             <  3:189959/149‑1 (MQ=255)
       tCGCATGAAGGGTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTcc                                                                                                                                            <  5:278019/149‑1 (MQ=255)
          ccTGAAGGGTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGc                                                                                                                                         >  1:367890/1‑149 (MQ=255)
                 ggTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGt                                                                                                                                  <  7:112102/149‑1 (MQ=255)
                  gTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTg                                                                                                                                 >  8:242404/1‑149 (MQ=255)
                           gCCTTCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGtt                                                                                                                        <  2:193647/149‑1 (MQ=255)
                           gCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGtt                                                                                                                        <  5:400165/149‑1 (MQ=255)
                                   cAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGc                                                                                                                                         <  1:32904/124‑1 (MQ=255)
                                   cAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGc                                                                                                                                         >  2:32904/1‑124 (MQ=255)
                                   cAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAg                                                                                                                 >  8:315193/1‑148 (MQ=255)
                                       gCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGc                                                                                                            <  2:130264/149‑1 (MQ=255)
                                         cTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCt                                                                                                          <  5:417819/149‑1 (MQ=255)
                                               ggtggtCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTg                                                                                                    >  5:659/1‑149 (MQ=255)
                                                gtggtCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGt                                                                                                   <  2:91552/149‑1 (MQ=255)
                                                gtggtCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGt                                                                                                   >  3:263085/1‑149 (MQ=255)
                                                  ggtCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCa                                                                                                 <  7:315193/149‑1 (MQ=255)
                                                   gtCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAg                                                                                                <  6:281794/149‑1 (MQ=255)
                                                      aTACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGc                                                                                             >  1:336057/1‑149 (MQ=255)
                                                                gCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGc                                                                                    >  3:115808/1‑148 (MQ=255)
                                                                  aGTTGGCTGCCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGAc                                                                                 <  7:242404/149‑1 (MQ=255)
                                                                                 gCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGa                                                                   <  2:367890/148‑1 (MQ=255)
                                                                                      cACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGc                                                             <  6:659/149‑1 (MQ=255)
                                                                                          gCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCt                                                                                       >  7:9283/1‑119 (MQ=255)
                                                                                          gCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCt                                                                                       <  8:9283/119‑1 (MQ=255)
                                                                                            ccGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATggg                                                       >  6:202017/1‑149 (MQ=255)
                                                                                             cGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATgggg                                                      <  4:263085/149‑1 (MQ=255)
                                                                                              gCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATgg                                                        >  3:103113/1‑146 (MQ=255)
                                                                                              gCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATgg                                                        <  4:103113/146‑1 (MQ=255)
                                                                                               cACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCg                                                    >  3:208431/1‑149 (MQ=255)
                                                                                                aCCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTgg                                                                                     <  8:27505/115‑1 (MQ=255)
                                                                                                       attCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCct                                            >  7:4841/2‑149 (MQ=255)
                                                                                                        ttCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCctg                                           >  4:380498/1‑149 (MQ=255)
                                                                                                           gcgGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAg                                                              <  1:133010/127‑1 (MQ=255)
                                                                                                           gcgGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAg                                                              >  2:133010/1‑127 (MQ=255)
                                                                                                           gcgGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCctgctg                                        <  2:336057/149‑1 (MQ=255)
                                                                                                            cgGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCtgctgc                                       <  8:4841/149‑1 (MQ=255)
                                                                                                             gggCCGCGAGCATGCCGTCCAGCAAATAACGTGGGCCAACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCtgctgc                                       >  4:132667/1‑148 (MQ=255)
                                                                                                             gggCCGCGAGCATGCCGTCCAGAGAATAACGTGGGCCAACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGAGGTACCCAtgctgc                                       >  8:219449/1‑148 (MQ=255)
                                                                                                               gCCGCTAGCATGCCGTCCAGCGAATAACGTGGGCCAACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCTCTGGCGACAAAGGCCTCGGCGACAATGCGATGGGGCGGTACCCCTGCTGCAAg                                    <  7:219449/149‑1 (MQ=255)
                                                                                                               gCCGCGAGCATGCCGTCCAGCAAATAACGTGGGCCAACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAg                                    <  3:132667/149‑1 (MQ=255)
                                                                                                                    gAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCAtt                               >  1:154266/1‑149 (MQ=255)
                                                                                                                      gCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGACTGAGGAACCCTTCGTTGTAAGTTCACCGCTGGCGACCAAGGCCTCCGCGGCCAAGCGAAGGGGGGGGTCCCCTTCTTCAAGGGAtttc                             >  1:320981/1‑147 (MQ=255)
                                                                                                                       cATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCt                            >  5:125847/1‑149 (MQ=255)
                                                                                                                        aTGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGGCAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGc                             >  5:367982/1‑147 (MQ=255)
                                                                                                                         tGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCTcg                          >  6:31323/1‑149 (MQ=255)
                                                                                                                                cAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGGTGCAAGGCATTGCTCGCGGGCCt                   >  6:121243/1‑149 (MQ=255)
                                                                                                                                  gCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCTCGCGGGCCTgg                 <  3:292872/149‑1 (MQ=255)
                                                                                                                                           gTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCTCGCGGGCCTGGCTGatcatc        <  5:31323/149‑1 (MQ=255)
                                                                                                                                                 cGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCTCGCGGGCCTGGCTGATCATCGTGGCa  <  6:125847/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGCCCATCGCCTGAAGGGTATGCGCGGCCATCTCCAGTGCCTGGGCGGTGGTCATACCGACCTGCAGTTGGCTGGCAATGGCAGCCACGGCCCGCACCGACTGTTCGCGGGCCGCGAGCATGCCGTCCAGCGAATAACGTGGGCCGACCTTTTCCAGCTCAGGGTGCAGCGGGTTGGCTGAGGAAGCCTGCGTTGTCAGTGCACCGCTGGCGACAAAGGCCTCGGCGACAAAGCGATGGGGCGGTACCCCTGCTGCAAGGCATTGCTCGCGGGCCTGGCTGATCATCGTGGCA  >  NC_002947/3025386‑3025679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑