Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,030,722 A→G 8.4% Q10R (CAG→CGG)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,030,7220AG 8.4% 160.1 / 4.8 59Q10R (CAG→CGG) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base G (3/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAG  >  NC_002947/3030581‑3030863
                                                                                                                                             |                                                                                                                                             
gcgAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAg                                                                                                                                        <  8:364499/149‑1 (MQ=255)
    gTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTG‑CTCCTGCCGCTGCGGCGGCACGacac                                                                                                                                   <  3:214163/149‑4 (MQ=255)
       gAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGca                                                                                                                                   >  8:157439/1‑147 (MQ=255)
          aGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCa                                                                                                                              <  5:412093/149‑1 (MQ=255)
           gTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCaa                                                                                                                             >  5:323669/1‑149 (MQ=255)
            ttCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAAc                                                                                                                            <  2:368583/149‑1 (MQ=255)
                cTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCTGCGGCGGCACGac                                                                                                                                     <  4:248704/136‑2 (MQ=255)
                cTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCTGCGGCGGCACGac                                                                                                                                     >  3:248704/1‑135 (MQ=255)
                 tGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGAc                                                                                                                       >  4:196552/1‑149 (MQ=255)
                  gCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGCCCCGACATGCCTTCGTTCCCCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGCCt                                                                                                                      >  8:195709/1‑149 (MQ=255)
                  gCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACt                                                                                                                      >  2:17299/1‑149 (MQ=255)
                   ccGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCAAAGCAACATGACTg                                                                                                                     >  7:94222/1‑149 (MQ=255)
                          aaCTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGa                                                                                                                                      <  5:413654/125‑1 (MQ=255)
                          aaCTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGa                                                                                                                                      >  6:413654/1‑125 (MQ=255)
                          aaCTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGcac                                                                                                                                  <  8:75410/129‑1 (MQ=255)
                          aaCTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGcac                                                                                                                                  >  7:75410/1‑129 (MQ=255)
                           aCTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATAc                                                                                                             <  6:224676/149‑1 (MQ=255)
                               ccGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAACACAGCAACATGACTGTCAAATACCGt                                                                                                          <  1:17299/148‑1 (MQ=255)
                                cGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTaa                                                                                                        <  4:232461/149‑1 (MQ=255)
                                cGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTaa                                                                                                        <  2:291378/149‑1 (MQ=255)
                                 gACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTaa                                                                                                        >  7:111975/1‑148 (MQ=255)
                                      tcGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACAcc                                                                                                    <  7:89145/147‑1 (MQ=255)
                                        ggCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCa                                                                                                 <  3:196552/148‑1 (MQ=255)
                                        ggCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCCTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCAt                                                                                                <  2:80973/149‑1 (MQ=255)
                                         gCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATc                                                                                               <  3:222795/149‑1 (MQ=255)
                                         gCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACAGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATc                                                                                               >  4:53789/1‑149 (MQ=255)
                                           aGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCgg                                                                                             >  3:266716/1‑149 (MQ=255)
                                              ggACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGcc                                                                                           >  8:274502/1‑148 (MQ=255)
                                                 cagacaACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAgg                                                                                       <  3:225665/149‑1 (MQ=255)
                                                 cagacaACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAgg                                                                                       <  3:53789/149‑1 (MQ=255)
                                                  agacaACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGc                                                                                      <  4:266716/149‑1 (MQ=255)
                                                      aaCTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCACATCGGCCAAGGCCGaa                                                                                  >  8:58374/1‑149 (MQ=255)
                                                       aCTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAAc                                                                                 <  5:375906/149‑1 (MQ=255)
                                                        cTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAAcg                                                                                >  1:103344/1‑149 (MQ=255)
                                                           tCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCga                                                                             <  8:111975/149‑1 (MQ=255)
                                                              gAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAATGCCGAACGCGAGAc                                                                          <  8:94222/149‑1 (MQ=255)
                                                              gAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGAc                                                                          >  3:271082/1‑149 (MQ=255)
                                                               aaaGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGAcc                                                                         >  6:252522/1‑149 (MQ=255)
                                                                  gAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGc                                                                      <  6:323669/149‑1 (MQ=255)
                                                                    aCCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCt                                                                    <  7:157439/149‑1 (MQ=255)
                                                                      cAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCgg                                                                                             >  2:34491/1‑122 (MQ=255)
                                                                      cAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCgg                                                                                             <  1:34491/122‑1 (MQ=255)
                                                                      cAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCaa                                                                                         <  4:199528/126‑1 (MQ=255)
                                                                      cAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCaa                                                                                         >  3:199528/1‑126 (MQ=255)
                                                                       aGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTc                                                                 >  1:114571/1‑149 (MQ=255)
                                                                        gCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCt                                                                    >  2:186496/1‑145 (MQ=255)
                                                                        gCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCt                                                                    <  1:186496/145‑1 (MQ=255)
                                                                                     aCCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGccc                                                   >  2:372264/1‑149 (MQ=255)
                                                                                               ccTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAgcgcg                                         <  7:103184/149‑1 (MQ=255)
                                                                                               ccTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAgcgc                                          <  5:252522/148‑1 (MQ=255)
                                                                                                          cGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTgg                              <  1:372264/149‑1 (MQ=255)
                                                                                                            ccTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCg                            <  4:351763/149‑1 (MQ=255)
                                                                                                                aGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGt                          <  4:271082/147‑1 (MQ=255)
                                                                                                                    aaaCGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCt                    >  8:299831/1‑149 (MQ=255)
                                                                                                                             tgtgtCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCt           >  6:316046/1‑149 (MQ=255)
                                                                                                                                 tcctGCCGCTGCGGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGAt       >  6:192833/4‑149 (MQ=255)
                                                                                                                                  cGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATg      >  4:345728/1‑149 (MQ=255)
                                                                                                                                   ctGCCGCTGCGGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGc     >  7:284968/2‑149 (MQ=255)
                                                                                                                                      ccgccgCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAg  <  7:274502/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGACCCGACATGCCTTCGTTCACCGCCTGAGTGAAACGTCTGTGTGTCGTGCCGCCGCAGCGGCAGGAGCACAGCAACATGACTGTCAAATACCGTAACACCTCATCGGCCAAGGCCGAACGCGAGACCCGCCTGTCCACCCGCGCCGCCCGTGAAGCGCGCAATGGCCTGGCGGTCACCCTCGCCAACCTCGATGCCAG  >  NC_002947/3030581‑3030863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑