Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,065,902	G→C	8.7%	G12G (GGG→GGC)	PP_2678 →	hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,065,902	0	G	C	8.7%	133.5 / 5.4	46	G12G (GGG→GGC)	PP_2678	hydrolase
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base C (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCATCGTCACCGACAGCG  >  NC_002947/3065768‑3066047                                                                                                                                        |                                                                                                                                                    gCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGCCGGCAGCCCCAGGc                                                                                                                                       <  6:125941/149‑1 (MQ=255)     acacGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGAt                                                                                                                                   >  4:215220/1‑149 (MQ=255)       acGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCt                                                                                                                                 >  6:284132/1‑149 (MQ=255)                 ttGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCgg                                                                                                                       >  8:131642/1‑149 (MQ=255)                    cGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCt                                                                                                                     >  5:57636/1‑148 (MQ=255)                    cGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCt                                                                                                                     <  3:246417/148‑1 (MQ=255)                    cGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTACCGGCCCAGGCTGATCTGGCGTACCGGCt                                                                                                                     <  6:351734/148‑1 (MQ=255)                            caGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCAc                                                                                                                  <  8:314354/143‑1 (MQ=255)                            caGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCAc                                                                                                                  >  7:314354/1‑143 (MQ=255)                            caGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCg                                                                                                            >  1:352632/1‑149 (MQ=255)                               cGGCTACCAGTTCGAGGCGGCGCTGCCGTGAGCGCGTTGTCTGATTTCACACTGTCCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GTGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCa                                                                                                         <  6:329899/149‑1 (MQ=255)                                 gCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGa                                                                                                       <  7:131642/149‑1 (MQ=255)                                   aaCCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCGCCACCCgg                                                                                                           >  1:140646/1‑143 (MQ=255)                                        gTTCGCGGCGGCGCTGCCCTGAGCGCGTTGGCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGa                                                                                                <  4:261892/149‑1 (MQ=255)                                         ttCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAg                                                                                               <  3:215220/149‑1 (MQ=255)                                                    gcTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGtt                                                                                    <  3:285314/149‑1 (MQ=255)                                                     cTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTg                                                                                   <  6:57636/149‑1 (MQ=255)                                                             tgcgcGTTGTCTGATTTCACCCTGAGCCGAGGCACCGTCATGCGTTGTATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAg                                                                            <  2:140646/147‑1 (MQ=255)                                                                           tttCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGa                                                                                                >  1:203998/1‑114 (MQ=255)                                                                           tttCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGa                                                                                                <  2:203998/114‑1 (MQ=255)                                                                            ttCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGgca                                                                         <  5:351268/136‑1 (MQ=255)                                                                            ttCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGgca                                                                         >  6:351268/1‑136 (MQ=255)                                                                            ttCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑ttt                                                          >  7:88479/1‑149 (MQ=255)                                                                              cACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTc                                                         <  1:55900/148‑1 (MQ=255)                                                                                 ccTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCTGGGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGc                                                       >  1:315242/1‑148 (MQ=255)                                                                                 ccTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGcc                                                      >  2:186043/1‑148 (MQ=255)                                                                                  cTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCAcccccc                                                                                                             >  1:336051/1‑94 (MQ=255)                                                                                  cTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCAcccccc                                                                                                             <  2:336051/94‑1 (MQ=255)                                                                                   tGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAgg                                                                           <  3:83000/127‑1 (MQ=255)                                                                                   tGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAgg                                                                           >  8:185795/1‑127 (MQ=255)                                                                                   tGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAgg                                                                           >  4:83000/1‑127 (MQ=255)                                                                                   tGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAgg                                                                           <  7:185795/127‑1 (MQ=255)                                                                                        cGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCaa                                              >  3:325088/1‑149 (MQ=255)                                                                                           ggCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATgg                                           <  2:315242/149‑1 (MQ=255)                                                                                                     tGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAAtgtg                                 <  2:352632/149‑1 (MQ=255)                                                                                                        gTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGt                               >  1:401361/1‑148 (MQ=255)                                                                                                              tCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACAcc                        >  6:210313/1‑149 (MQ=255)                                                                                                              tCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACAcc                        >  7:252382/1‑149 (MQ=255)                                                                                                                     gTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCa                 >  1:15819/1‑149 (MQ=255)                                                                                                                            ctgTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGTCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCATCGTCa           <  5:284132/148‑1 (MQ=255)                                                                                                                            ctgTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCATCGTc            <  2:15819/147‑1 (MQ=255)                                                                                                                              gtgCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGAGAGCACCGATGAGGTTTCGCCAAGGCCAATGGGGGCAATGTGGTCAACACCGCGTTCATCGTCAc          >  1:334924/1‑149 (MQ=255)                                                                                                                                gCCT‑GGGCCGGCAGCCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAaca                          <  3:23776/129‑1 (MQ=255)                                                                                                                                gCCT‑GGGCCGGCAGCCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAaca                          >  4:23776/1‑129 (MQ=255)                                                                                                                                gCCT‑GGGCCGGCAGCCCCAGGCTGATCTGGCGTACCGGCTCAACCCCCGGCAGATCGCCGAGGGCACCTCGTTGCTGGAAGGCAGCACAGACAA‑‑TTTCGACAAGGCCAATGGCGGCAATGTGGTCATCACGGCGTTCATCGTCAccaa       >  7:65242/1‑146 (MQ=255)                                                                                                                                  cT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCATCGTCACCGACAg    >  7:151066/1‑149 (MQ=255)                                                                                                                                     ggggCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGATGGCAGCACCGCCAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCATCGTCACCGACAGCg  >  3:27830/1‑149 (MQ=255)                                                                                                                                        |                                                                                                                                                    GCCAACACGGCTGCTGTTGCGGGACAACAGCGGCAACCAGTTCGAGGCGGCGCTGCCCTGAGCGCGTTGTCTGATTTCACCCTGACCCGAGGCACCGTCATGCGTTGGATCCTGCTGTTGCTGCTGTGCCT‑GGGGCTGCCGGCCCAGGCTGATCTGGCGTACCGGCTCACCCCCCGGCAGATCGCCGAGGGCACCTGGTTGCTGGAAGGCAGCACCGACAA‑‑TTTCGCCAAGGCCAATGGCGGCAATGTGGTCAACACCGCGTTCATCGTCACCGACAGCG  >  NC_002947/3065768‑3066047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑