Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,067,430 G→T 7.5% A157S (GCC→TCC)  qedH‑II → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,067,4300GT 7.5% 165.8 / 3.8 53A157S (GCC→TCC) qedH‑IIquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

GCCGTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGT  >  NC_002947/3067283‑3067569
                                                                                                                                                   |                                                                                                                                           
gCCGTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGc                                                                                                                                            >  5:336188/1‑149 (MQ=255)
 ccGTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGGCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGcc                                                                                                                                           >  2:290828/1‑149 (MQ=255)
 ccGTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGcc                                                                                                                                           >  2:106541/1‑149 (MQ=255)
  cGTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGccc                                                                                                                                          <  1:62752/149‑1 (MQ=255)
   gTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCt                                                                                                                                         <  1:281442/149‑1 (MQ=255)
         gCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAAc                                                                                                                                     >  6:380068/1‑147 (MQ=255)
          cccGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACaa                                                                                                                                   <  8:36003/148‑1 (MQ=255)
           ccGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAg                                                                                                                                  >  3:293554/1‑148 (MQ=255)
               aCCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGacac                                                                                                                             <  4:293554/149‑1 (MQ=255)
                     aaGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCCGGTGTCCTTGTTCAGGGCCACCAGctt                                                                                                                       >  8:1207/1‑147 (MQ=255)
                      aGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCCGGTGTCCTTGTTCAGGGCCACCAGcttg                                                                                                                      <  7:1207/149‑4 (MQ=255)
                        aaaCTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAggt                                                                                                                    >  3:34434/1‑149 (MQ=255)
                         aaCTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAggtg                                                                                                                   <  6:336188/149‑1 (MQ=255)
                              tgGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTg                                                                                                              <  1:106541/149‑1 (MQ=255)
                                        aTGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                    <  5:101690/149‑1 (MQ=255)
                                          gCACGTCTGCCGGATGACATCCGCCCCTGCTGCGCCGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGt                                                                                                  >  8:275012/1‑149 (MQ=255)
                                           cACGTCTGGCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTc                                                                                                 <  1:290828/149‑1 (MQ=255)
                                               tCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGTTCGCCg                                                                                             >  3:6193/1‑149 (MQ=255)
                                                   ccGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAcc                                                                                          <  5:380068/148‑1 (MQ=255)
                                                     ggATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGCCcac                                                                                        >  1:162031/1‑148 (MQ=255)
                                                         gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                    <  3:213679/132‑1 (MQ=255)
                                                         gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                    >  4:213679/1‑132 (MQ=255)
                                                         gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaag                                                                                    >  1:351877/1‑148 (MQ=255)
                                                          aCATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                  <  4:34434/149‑1 (MQ=255)
                                                          aCATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                  <  8:240990/149‑1 (MQ=255)
                                                              ccGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGc                                                                               >  3:92418/1‑148 (MQ=255)
                                                                             cGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAc                                                                                           <  5:120666/121‑1 (MQ=255)
                                                                             cGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAc                                                                                           >  6:120666/1‑121 (MQ=255)
                                                                                aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAg                                                                                                                      <  6:176496/91‑1 (MQ=255)
                                                                                aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAg                                                                                                                      >  5:176496/1‑91 (MQ=255)
                                                                                aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                  <  4:335992/127‑1 (MQ=255)
                                                                                aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                  >  3:335992/1‑127 (MQ=255)
                                                                                  tCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGTCCTTGTTCAGGGCCACCAGc                                                                                                                         >  6:402471/1‑86 (MQ=37)
                                                                                  tCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGTCCTTGTTCAGGGCCACCAGc                                                                                                                         <  5:402471/86‑1 (MQ=37)
                                                                                        gcggcgTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGGCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATc                                                    <  6:26830/149‑1 (MQ=255)
                                                                                          ggcgTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGt                                                  <  5:92538/149‑1 (MQ=255)
                                                                                              tCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAAt                                              <  6:313611/149‑1 (MQ=255)
                                                                                                gcgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATgg                                            <  2:351877/149‑1 (MQ=255)
                                                                                                gcgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATgg                                            >  4:260546/1‑149 (MQ=255)
                                                                                                 cgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGc                                           <  8:241746/149‑1 (MQ=255)
                                                                                                                  gtgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGc                            <  4:92418/147‑1 (MQ=255)
                                                                                                                   tgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTTGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGtt                         <  4:6193/149‑1 (MQ=255)
                                                                                                                       cttcGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCcg                     >  8:252621/1‑149 (MQ=255)
                                                                                                                          cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                <  7:273683/135‑1 (MQ=255)
                                                                                                                          cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                >  8:273683/1‑135 (MQ=255)
                                                                                                                          cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                >  8:279161/1‑135 (MQ=255)
                                                                                                                          cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGAACGTCAATGGCAAGCTGATcac                                <  7:279161/135‑1 (MQ=255)
                                                                                                                           ggCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCcggcgg                 >  4:219846/1‑149 (MQ=255)
                                                                                                                             cACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCggcggc                >  6:9443/1‑148 (MQ=255)
                                                                                                                             cACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCggcggc                >  2:127397/1‑148 (MQ=255)
                                                                                                                               cGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAg             <  1:127397/149‑1 (MQ=255)
                                                                                                                                     aCGCCAAGCTGGTGGCCCTGAACAAGGGCACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGc       >  1:194042/1‑149 (MQ=255)
                                                                                                                                          aaGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCgtggt  >  1:162657/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GCCGTGGATGCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGT  >  NC_002947/3067283‑3067569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑