Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,067,436 A→T 8.3% N159Y (AAC→TAC) ‡ qedH‑II → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,067,4360AT 8.3% 136.0 / 3.3 48N159Y (AAC→TAC) ‡qedH‑IIquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (21/23);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

GCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGC  >  NC_002947/3067292‑3067584
                                                                                                                                                |                                                                                                                                                    
gCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAAc                                                                                                                                                    >  6:380068/1‑147 (MQ=255)
 cccGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACaa                                                                                                                                                  <  8:36003/148‑1 (MQ=255)
  ccGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAg                                                                                                                                                 >  3:293554/1‑148 (MQ=255)
      aCCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGacac                                                                                                                                            <  4:293554/149‑1 (MQ=255)
            aaGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCCGGTGTCCTTGTTCAGGGCCACCAGctt                                                                                                                                      >  8:1207/1‑147 (MQ=255)
             aGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCCGGTGTCCTTGTTCAGGGCCACCAGcttg                                                                                                                                     <  7:1207/149‑4 (MQ=255)
               aaaCTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAggt                                                                                                                                   >  3:34434/1‑149 (MQ=255)
                aaCTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAggtg                                                                                                                                  <  6:336188/149‑1 (MQ=255)
                     tgGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTg                                                                                                                             <  1:106541/149‑1 (MQ=255)
                               aTGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                                   <  5:101690/149‑1 (MQ=255)
                                 gCACGTCTGCCGGATGACATCCGCCCCTGCTGCGCCGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGt                                                                                                                 >  8:275012/1‑149 (MQ=255)
                                  cACGTCTGGCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTc                                                                                                                <  1:290828/149‑1 (MQ=255)
                                      tCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGTTCGCCg                                                                                                            >  3:6193/1‑149 (MQ=255)
                                          ccGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAcc                                                                                                         <  5:380068/148‑1 (MQ=255)
                                            ggATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGCCcac                                                                                                       >  1:162031/1‑148 (MQ=255)
                                                gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                                   <  3:213679/132‑1 (MQ=255)
                                                gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                                   >  4:213679/1‑132 (MQ=255)
                                                gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaag                                                                                                   >  1:351877/1‑148 (MQ=255)
                                                 aCATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                 <  8:240990/149‑1 (MQ=255)
                                                 aCATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                 <  4:34434/149‑1 (MQ=255)
                                                     ccGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGc                                                                                              >  3:92418/1‑148 (MQ=255)
                                                                    cGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAc                                                                                                          <  5:120666/121‑1 (MQ=255)
                                                                    cGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAc                                                                                                          >  6:120666/1‑121 (MQ=255)
                                                                       aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAg                                                                                                                                     >  5:176496/1‑91 (MQ=255)
                                                                       aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAg                                                                                                                                     <  6:176496/91‑1 (MQ=255)
                                                                       aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                 <  4:335992/127‑1 (MQ=255)
                                                                       aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                 >  3:335992/1‑127 (MQ=255)
                                                                         tCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGTCCTTGTTCAGGGCCACCAGc                                                                                                                                        <  5:402471/86‑1 (MQ=37)
                                                                         tCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGTCCTTGTTCAGGGCCACCAGc                                                                                                                                        >  6:402471/1‑86 (MQ=37)
                                                                               gcggcgTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGGCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATc                                                                   <  6:26830/149‑1 (MQ=255)
                                                                                 ggcgTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGt                                                                 <  5:92538/149‑1 (MQ=255)
                                                                                     tCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAAt                                                             <  6:313611/149‑1 (MQ=255)
                                                                                       gcgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATgg                                                           >  4:260546/1‑149 (MQ=255)
                                                                                       gcgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATgg                                                           <  2:351877/149‑1 (MQ=255)
                                                                                        cgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGc                                                          <  8:241746/149‑1 (MQ=255)
                                                                                                         gtgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGc                                           <  4:92418/147‑1 (MQ=255)
                                                                                                          tgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTTGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGtt                                        <  4:6193/149‑1 (MQ=255)
                                                                                                              cttcGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCcg                                    >  8:252621/1‑149 (MQ=255)
                                                                                                                 cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                               <  7:273683/135‑1 (MQ=255)
                                                                                                                 cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                               >  8:273683/1‑135 (MQ=255)
                                                                                                                 cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                               >  8:279161/1‑135 (MQ=255)
                                                                                                                 cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGAACGTCAATGGCAAGCTGATcac                                               <  7:279161/135‑1 (MQ=255)
                                                                                                                  ggCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCcggcgg                                >  4:219846/1‑149 (MQ=255)
                                                                                                                    cACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCggcggc                               >  6:9443/1‑148 (MQ=255)
                                                                                                                    cACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCggcggc                               >  2:127397/1‑148 (MQ=255)
                                                                                                                      cGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAg                            <  1:127397/149‑1 (MQ=255)
                                                                                                                            aCGCCAAGCTGGTGGCCCTGAACAAGGGCACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGc                      >  1:194042/1‑149 (MQ=255)
                                                                                                                                 aaGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCgtggt                 >  1:162657/1‑149 (MQ=255)
                                                                                                                                                aaCAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAg    <  5:9443/147‑1 (MQ=255)
                                                                                                                                                aaCAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGc  >  2:202303/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCCCGCACCGGCAAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGC  >  NC_002947/3067292‑3067584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑