Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,067,448 G→A 7.4% G163S (GGC→AGC)  qedH‑II → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,067,4480GA 7.4% 151.3 / 2.5 54G163S (GGC→AGC) qedH‑IIquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

AAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAACCCGG  >  NC_002947/3067304‑3067595
                                                                                                                                                |                                                                                                                                                   
aaGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCCGGTGTCCTTGTTCAGGGCCACCAGctt                                                                                                                                                 >  8:1207/1‑147 (MQ=255)
 aGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCCGGTGTCCTTGTTCAGGGCCACCAGcttg                                                                                                                                                <  7:1207/149‑4 (MQ=255)
   aaaCTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAggt                                                                                                                                              >  3:34434/1‑149 (MQ=255)
    aaCTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAggtg                                                                                                                                             <  6:336188/149‑1 (MQ=255)
         tgGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTg                                                                                                                                        <  1:106541/149‑1 (MQ=255)
                   aTGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                                              <  5:101690/149‑1 (MQ=255)
                     gCACGTCTGCCGGATGACATCCGCCCCTGCTGCGCCGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGt                                                                                                                            >  8:275012/1‑149 (MQ=255)
                      cACGTCTGGCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTc                                                                                                                           <  1:290828/149‑1 (MQ=255)
                          tCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGTTCGCCg                                                                                                                       >  3:6193/1‑149 (MQ=255)
                              ccGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAcc                                                                                                                    <  5:380068/148‑1 (MQ=255)
                                ggATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGCCcac                                                                                                                  >  1:162031/1‑148 (MQ=255)
                                    gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                                              <  3:213679/132‑1 (MQ=255)
                                    gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCaagaag                                                                                                                              >  4:213679/1‑132 (MQ=255)
                                    gACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaag                                                                                                              >  1:351877/1‑148 (MQ=255)
                                     aCATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                            <  8:240990/149‑1 (MQ=255)
                                     aCATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                            <  4:34434/149‑1 (MQ=255)
                                         ccGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGc                                                                                                         >  3:92418/1‑148 (MQ=255)
                                                        cGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAc                                                                                                                     >  6:120666/1‑121 (MQ=255)
                                                        cGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGAc                                                                                                                     <  5:120666/121‑1 (MQ=255)
                                                           aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAg                                                                                                                                                <  6:176496/91‑1 (MQ=255)
                                                           aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAg                                                                                                                                                >  5:176496/1‑91 (MQ=255)
                                                           aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                            >  3:335992/1‑127 (MQ=255)
                                                           aaTCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAaagaa                                                                                                            <  4:335992/127‑1 (MQ=255)
                                                             tCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGTCCTTGTTCAGGGCCACCAGc                                                                                                                                                   >  6:402471/1‑86 (MQ=37)
                                                             tCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGTCCTTGTTCAGGGCCACCAGc                                                                                                                                                   <  5:402471/86‑1 (MQ=37)
                                                                   gcggcgTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGGCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATc                                                                              <  6:26830/149‑1 (MQ=255)
                                                                     ggcgTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGt                                                                            <  5:92538/149‑1 (MQ=255)
                                                                         tCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAAt                                                                        <  6:313611/149‑1 (MQ=255)
                                                                           gcgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATgg                                                                      <  2:351877/149‑1 (MQ=255)
                                                                           gcgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATgg                                                                      >  4:260546/1‑149 (MQ=255)
                                                                            cgcTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGc                                                                     <  8:241746/149‑1 (MQ=255)
                                                                                             gtgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGc                                                      <  4:92418/147‑1 (MQ=255)
                                                                                              tgtTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTTGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGtt                                                   <  4:6193/149‑1 (MQ=255)
                                                                                                  cttcGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCcg                                               >  8:252621/1‑149 (MQ=255)
                                                                                                     cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                                          >  8:273683/1‑135 (MQ=255)
                                                                                                     cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                                          <  7:273683/135‑1 (MQ=255)
                                                                                                     cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATcac                                                          >  8:279161/1‑135 (MQ=255)
                                                                                                     cGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGAACGTCAATGGCAAGCTGATcac                                                          <  7:279161/135‑1 (MQ=255)
                                                                                                      ggCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCcggcgg                                           >  4:219846/1‑149 (MQ=255)
                                                                                                        cACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCggcggc                                          >  2:127397/1‑148 (MQ=255)
                                                                                                        cACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCggcggc                                          >  6:9443/1‑148 (MQ=255)
                                                                                                          cGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAg                                       <  1:127397/149‑1 (MQ=255)
                                                                                                                aCGCCAAGCTGGTGGCCCTGAACAAGGGCACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGc                                 >  1:194042/1‑149 (MQ=255)
                                                                                                                     aaGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCgtggt                            >  1:162657/1‑149 (MQ=255)
                                                                                                                                    atcAAGGACACCGGCAAGGTGGTCTGGAGCAAGATGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAg               <  3:266619/145‑1 (MQ=255)
                                                                                                                                    aaCAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAg               <  5:9443/147‑1 (MQ=255)
                                                                                                                                    aaCAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGc             >  2:202303/1‑149 (MQ=255)
                                                                                                                                       aaGGACACCGGCAAGGAGGTCTGGAGCAAGAAGGTCGCCGCCCACAAAGAAGGCTACTCCATCAGCGCCGCGCAGATGATCGTCAATGGCACGCTGATCACAGGCGTTGACGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGCCGTa          >  6:93923/1‑149 (MQ=255)
                                                                                                                                           acacCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAAc      >  2:372298/1‑149 (MQ=255)
                                                                                                                                             acCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAAccc    >  2:306752/1‑149 (MQ=255)
                                                                                                                                              ccGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAACCCg   <  3:219846/149‑1 (MQ=255)
                                                                                                                                               cGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAACCCgg  <  7:252621/149‑1 (MQ=255)
                                                                                                                                               cGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAACCCg   >  7:348645/1‑148 (MQ=255)
                                                                                                                                               cGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGCTGGGCAAGATCCAGGCGTACAACCCg   >  8:167661/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AAGAAACTGTGGCAATACGATGCACGTCTGCCGGATGACATCCGCCCCTGCTGCGACGTAATCAACCGCGGCGTCGCGCTGTACGGCAACCTGGTGTTCTTCGGCACGCTGGACGCCAAGCTGGTGGCCCTGAACAAGGACACCGGCAAGGTGGTCTGGAGCAAGAAGGTCGCCGACCACAAAGAAGGCTACTCCATCAGCGCCGCGCCGATGATCGTCAATGGCAAGCTGATCACAGGCGTTGCCGGCGGCGAGTTCGGCGTGGTGGGCAAGATCCAGGCGTACAACCCGG  >  NC_002947/3067304‑3067595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑