Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,105,669 G→C 12.0% L123V (CTG→GTG)  PP_2716 ← arsenate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,105,6690GC12.0% 111.4 / 7.7 42L123V (CTG→GTG) PP_2716arsenate reductase
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base C (2/3);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

GAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGT  >  NC_002947/3105522‑3105815
                                                                                                                                                   |                                                                                                                                                  
gAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAgt                                                                                                                                                   >  4:168629/1‑149 (MQ=255)
 aGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGTAGGCCTGGCAGCGAGACTCGATACGGGCCAgtg                                                                                                                                                  <  1:260556/149‑1 (MQ=255)
    tCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCCTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTgg                                                                                                                                               >  7:377329/1‑149 (MQ=255)
          ttAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAAAGGCAGCGCGAAGAAGGCCTGGCAGCGAGCCTCGATACGGGCCAGTGTGGCCCgg                                                                                                                                          >  8:375738/1‑148 (MQ=255)
              gCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCACACTGGCCCgtatcga                                                                                                                                     <  6:186208/149‑7 (MQ=255)
              gCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCACACTGGCCCgtatcga                                                                                                                                     <  4:198204/149‑7 (MQ=255)
                    gCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCAt                                                                                                                                <  7:206368/148‑1 (MQ=255)
                     cTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATc                                                                                                                               <  3:366470/148‑1 (MQ=255)
                      tCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGa                                                                                                                             <  8:296911/149‑1 (MQ=255)
                        ggACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGAcc                                                                                                                           >  2:313039/1‑149 (MQ=255)
                         gaaaTGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTAGATACGGGCCAGTGTGGCCCGGAAAGCCTCATCGACCg                                                                                                                          >  8:188792/4‑149 (MQ=255)
                         gACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCg                                                                                                                          >  8:194195/1‑149 (MQ=255)
                                  tcttcCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCg                                                                                                                                           <  7:192150/123‑1 (MQ=255)
                                  tcttcCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCg                                                                                                                                           >  8:192150/1‑123 (MQ=255)
                                          ttCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCAc                                                                                                          >  5:323021/1‑148 (MQ=255)
                                          ttCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCAc                                                                                                          >  6:66212/1‑148 (MQ=255)
                                            cAAAGCGAGCCGATGCGATCCAGCGCGTGATTGGGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGa                                                                                                        >  1:235335/1‑148 (MQ=255)
                                               aGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATc                                                                                                    >  3:105607/1‑149 (MQ=255)
                                                     ccGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAggg                                                                                              <  3:168629/149‑1 (MQ=255)
                                                           cGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTc                                                                                        <  1:313039/149‑1 (MQ=255)
                                                            gATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTc                                                                                        >  6:409550/1‑148 (MQ=255)
                                                             aTCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCa                                                                                      >  3:101657/1‑149 (MQ=255)
                                                                  gcgcGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGcccc                                                                                 <  6:66507/149‑1 (MQ=255)
                                                                    gcgTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCa                                                                               <  5:66212/149‑1 (MQ=255)
                                                                     cgTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCaa                                                                              <  2:80394/149‑1 (MQ=255)
                                                                            gAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGa                                                                         <  5:409550/147‑1 (MQ=255)
                                                                              gctgctCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTc                                                                     <  4:105607/149‑1 (MQ=255)
                                                                                  ctCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAAc                                                                  <  8:377329/148‑1 (MQ=255)
                                                                                        gTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCGACGACATCGGAGGTGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGc                                                           <  7:375738/149‑1 (MQ=255)
                                                                                           gAGGTGATCGAACGGCAGCGCGTAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCg                                                         <  6:37188/148‑1 (MQ=255)
                                                                                              gTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGaaa                                                      <  4:178412/148‑1 (MQ=255)
                                                                                              gTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAAt                                                     <  7:194195/149‑1 (MQ=255)
                                                                                               tGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCATTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCAACCACGACATCGGAGGTGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAAt                                                     <  7:188792/148‑1 (MQ=255)
                                                                                                          cAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGc                                                                           >  7:171089/1‑115 (MQ=255)
                                                                                                          cAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGc                                                                           <  8:171089/115‑1 (MQ=255)
                                                                                                                gaagaaGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGc                                   >  8:345665/1‑149 (MQ=255)
                                                                                                                            gCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGt                       >  2:165019/1‑149 (MQ=255)
                                                                                                                                      tCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTc                      <  7:320576/140‑1 (MQ=255)
                                                                                                                                      tCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTc                      >  8:320576/1‑140 (MQ=255)
                                                                                                                                        tttccGGGCCACACTGGCCCGTAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGcc                                                          >  8:185429/5‑102 (MQ=255)
                                                                                                                                        tttccGGGCCACACTGGCCCGTAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGcc                                                          <  7:185429/98‑1 (MQ=255)
                                                                                                                                        tttccGGGCCACACTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGtga           >  1:124909/5‑149 (MQ=255)
                                                                                                                                        gATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGtg            <  8:78552/148‑1 (MQ=255)
                                                                                                                                                 cAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGt  >  7:136781/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGT  >  NC_002947/3105522‑3105815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑