Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,105,671 G→C 11.9% T122R (ACA→AGA) ‡ PP_2716 ← arsenate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,105,6710GC11.9% 120.3 / 7.7 42T122R (ACA→AGA) ‡PP_2716arsenate reductase
Reads supporting (aligned to +/- strand):  ref base G (17/20);  new base C (2/3);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

AGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGTCGG  >  NC_002947/3105523‑3105818
                                                                                                                                                    |                                                                                                                                                   
aGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGTAGGCCTGGCAGCGAGACTCGATACGGGCCAgtg                                                                                                                                                     <  1:260556/149‑1 (MQ=255)
   tCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCCTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTgg                                                                                                                                                  >  7:377329/1‑149 (MQ=255)
         ttAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAAAGGCAGCGCGAAGAAGGCCTGGCAGCGAGCCTCGATACGGGCCAGTGTGGCCCgg                                                                                                                                             >  8:375738/1‑148 (MQ=255)
             gCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCACACTGGCCCgtatcga                                                                                                                                        <  6:186208/149‑7 (MQ=255)
             gCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCACACTGGCCCgtatcga                                                                                                                                        <  4:198204/149‑7 (MQ=255)
                   gCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCAt                                                                                                                                   <  7:206368/148‑1 (MQ=255)
                    cTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATc                                                                                                                                  <  3:366470/148‑1 (MQ=255)
                     tCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGa                                                                                                                                <  8:296911/149‑1 (MQ=255)
                       ggACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGAcc                                                                                                                              >  2:313039/1‑149 (MQ=255)
                        gaaaTGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTAGATACGGGCCAGTGTGGCCCGGAAAGCCTCATCGACCg                                                                                                                             >  8:188792/4‑149 (MQ=255)
                        gACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCg                                                                                                                             >  8:194195/1‑149 (MQ=255)
                                 tcttcCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCg                                                                                                                                              <  7:192150/123‑1 (MQ=255)
                                 tcttcCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCg                                                                                                                                              >  8:192150/1‑123 (MQ=255)
                                         ttCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCAc                                                                                                             >  6:66212/1‑148 (MQ=255)
                                         ttCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCAc                                                                                                             >  5:323021/1‑148 (MQ=255)
                                           cAAAGCGAGCCGATGCGATCCAGCGCGTGATTGGGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGa                                                                                                           >  1:235335/1‑148 (MQ=255)
                                              aGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATc                                                                                                       >  3:105607/1‑149 (MQ=255)
                                                    ccGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAggg                                                                                                 <  3:168629/149‑1 (MQ=255)
                                                          cGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTc                                                                                           <  1:313039/149‑1 (MQ=255)
                                                           gATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTc                                                                                           >  6:409550/1‑148 (MQ=255)
                                                            aTCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCa                                                                                         >  3:101657/1‑149 (MQ=255)
                                                                 gcgcGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGcccc                                                                                    <  6:66507/149‑1 (MQ=255)
                                                                   gcgTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCa                                                                                  <  5:66212/149‑1 (MQ=255)
                                                                    cgTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCaa                                                                                 <  2:80394/149‑1 (MQ=255)
                                                                           gAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGa                                                                            <  5:409550/147‑1 (MQ=255)
                                                                             gctgctCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTc                                                                        <  4:105607/149‑1 (MQ=255)
                                                                                 ctCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAAc                                                                     <  8:377329/148‑1 (MQ=255)
                                                                                       gTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCGACGACATCGGAGGTGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGc                                                              <  7:375738/149‑1 (MQ=255)
                                                                                          gAGGTGATCGAACGGCAGCGCGTAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCg                                                            <  6:37188/148‑1 (MQ=255)
                                                                                             gTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGaaa                                                         <  4:178412/148‑1 (MQ=255)
                                                                                             gTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAAt                                                        <  7:194195/149‑1 (MQ=255)
                                                                                              tGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCATTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCAACCACGACATCGGAGGTGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAAt                                                        <  7:188792/148‑1 (MQ=255)
                                                                                                         cAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGc                                                                              >  7:171089/1‑115 (MQ=255)
                                                                                                         cAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGc                                                                              <  8:171089/115‑1 (MQ=255)
                                                                                                               gaagaaGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGc                                      >  8:345665/1‑149 (MQ=255)
                                                                                                                           gCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGt                          >  2:165019/1‑149 (MQ=255)
                                                                                                                                     tCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTc                         <  7:320576/140‑1 (MQ=255)
                                                                                                                                     tCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTc                         >  8:320576/1‑140 (MQ=255)
                                                                                                                                       tttccGGGCCACACTGGCCCGTAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGcc                                                             >  8:185429/5‑102 (MQ=255)
                                                                                                                                       tttccGGGCCACACTGGCCCGTAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGcc                                                             <  7:185429/98‑1 (MQ=255)
                                                                                                                                       tttccGGGCCACACTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGtga              >  1:124909/5‑149 (MQ=255)
                                                                                                                                       gATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGtg               <  8:78552/148‑1 (MQ=255)
                                                                                                                                                cAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGt     >  7:136781/1‑149 (MQ=255)
                                                                                                                                                   tgtgGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGTCgg  >  3:289401/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGTCGAGGTTAGGCAGTTGCTCGGACATGTTTTCTTCCTGTTCAAAGCGAGCCGATGCGATCCAGCGCGTGCTTGAGCTGCTCGCGGTCGAGGTGATCGAACGGCAGCGCGAAGAAGGCCTGGCAGCGAGACTCGATACGGGCCAGTGTGGCCCGGAAAGCCGCATCGACCGTAGCTTCGTCACCCACGACATCGGAGGGGTCTTCCAGCCCCCAATGCGACTTCAACGCAGGGCCGAAATACACCGGGCAGGCTTCGCCCGCTGCCTTGTCGCAGACGGTGATGACGATGTCGG  >  NC_002947/3105523‑3105818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑