Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,112,857 A→G 8.2% L97P (CTT→CCT) ‡ PP_2727 ← C factor cell‑cell signaling protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,112,8570AG 8.2% 129.0 / 2.5 50L97P (CTT→CCT) ‡PP_2727C factor cell‑cell signaling protein
Reads supporting (aligned to +/- strand):  ref base A (17/27);  new base G (2/2);  total (19/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

CGGGTGCCGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAACAAGGTATCCAGCGCAGGGC  >  NC_002947/3112712‑3113001
                                                                                                                                                  |                                                                                                                                                
cGGGTGCCGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGATACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCg                                                                                                                                               >  4:280206/1‑149 (MQ=255)
  ggTGCCGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACGGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTg                                                                                                                                             <  8:333028/149‑1 (MQ=255)
  ggTGCCGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTg                                                                                                                                             >  7:2268/1‑149 (MQ=255)
      ccGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGa                                                                                                                                         <  2:381989/149‑1 (MQ=255)
      ccGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGATGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACTCTTGGCGTGCTgc                                                                                                                                         <  6:292316/149‑2 (MQ=255)
       cGACGCTGCAGCGGCTGCAAATGCTTGTGCAGCAGATCAGGGGCGAAACAGTGGGGTGTGAAACTTCTCAGCAACCGCGCCATGTCTAGCTGGGTCTGTGCTTTTTCAG‑GCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGaa                                                                                                                                        <  4:97322/149‑1 (MQ=255)
           gcagcaGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGAcc                                                                                                                                    <  5:151279/149‑1 (MQ=255)
              gcagcGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCATGCGTGCTGAAGACCAgg                                                                                                                                 <  4:333878/149‑1 (MQ=255)
                 gcGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTc                                                                                                                              <  3:383697/149‑1 (MQ=255)
                       gcaAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTgg                                                                                                                        <  4:350324/149‑1 (MQ=255)
                         aaaTGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAGCCCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGt                                                                                                                       <  1:309886/149‑1 (MQ=255)
                          aaTGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCg                                                                                                                     <  3:354964/149‑1 (MQ=255)
                          aaTGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCg                                                                                                                     >  4:354964/1‑149 (MQ=255)
                               ttGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCgcag                                                                                                                  >  3:347502/1‑147 (MQ=255)
                                    cagcagAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGgcgc                                                                                                           <  7:21933/149‑1 (MQ=255)
                                          aaCAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCt                                                                                                     <  7:196146/149‑1 (MQ=255)
                                              ggggCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTgcgc                                                                                                 <  4:347502/149‑1 (MQ=255)
                                                   gAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCACGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCa                                                                                            <  4:218622/149‑1 (MQ=255)
                                                   gAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGc                                                                                              >  2:288091/1‑147 (MQ=255)
                                                         gttggttgTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGttt                                                                                      <  6:293645/149‑1 (MQ=255)
                                                            ggttgTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGc                                                                                    <  6:217212/148‑1 (MQ=255)
                                                              ttgTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGt                                                                                 <  5:413535/149‑1 (MQ=255)
                                                              ttgTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGt                                                                                 <  3:304169/149‑1 (MQ=255)
                                                                 tgAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGc                                                                               <  2:361219/148‑1 (MQ=255)
                                                                   aaaCTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTg                                                                            >  8:311369/1‑149 (MQ=255)
                                                                        gCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCg                                                                        >  1:279817/1‑148 (MQ=255)
                                                                          tCAGCACCCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCCAGCGTGCTGAAGCCCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGAt                                                                     >  3:361507/1‑149 (MQ=255)
                                                                             gCAACCCCGCCATGTCTAGCTGGGGCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCgg                                                                  <  8:2268/149‑1 (MQ=255)
                                                                                           tCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCCCGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGa                                                     <  6:34325/148‑1 (MQ=255)
                                                                                           tCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGa                                                     <  5:211811/148‑1 (MQ=255)
                                                                                           tCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGa                                                     <  3:280206/148‑1 (MQ=255)
                                                                                                cTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACGAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTGGCAGTCGATGCGaa                                               <  8:300753/149‑1 (MQ=255)
                                                                                                     tCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGaa                                               <  5:321547/144‑1 (MQ=255)
                                                                                                     tCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGaa                                               >  6:321547/1‑144 (MQ=255)
                                                                                                        gTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGt                                       >  4:269994/1‑149 (MQ=255)
                                                                                                           cTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTcgc                                    >  1:365422/1‑149 (MQ=255)
                                                                                                              tttCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTcgcgcg                                 >  7:318712/1‑149 (MQ=255)
                                                                                                                  aG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGaacca                                            >  4:192034/1‑134 (MQ=255)
                                                                                                                  aG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGaacca                                            <  3:192034/134‑1 (MQ=255)
                                                                                                                  aG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGct                             >  2:181377/1‑149 (MQ=255)
                                                                                                                      ccGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCtg                          <  2:279817/149‑1 (MQ=255)
                                                                                                                           ccGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAAc                     <  5:126860/149‑1 (MQ=255)
                                                                                                                           ccGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAAc                     <  8:318712/149‑1 (MQ=255)
                                                                                                                                 cttcttGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGc                                                                                    <  6:125170/80‑1 (MQ=255)
                                                                                                                                 cttcttGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGc                                                                                    >  5:125170/1‑80 (MQ=255)
                                                                                                                                     cttcagcaCGCTTGGCGTGCTGCAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAACAAGGTATc             >  2:192105/4‑147 (MQ=255)
                                                                                                                                       gcagcaCGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAACAAGGTATCCAgc         >  8:348361/1‑149 (MQ=255)
                                                                                                                                        cagcaCGCTTGGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAAGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCtgtg                        <  2:216432/133‑1 (MQ=255)
                                                                                                                                        cagcaCGCTTGGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAAGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCtgtg                        >  1:216432/1‑133 (MQ=255)
                                                                                                                                              gCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGAAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAACAAGGTATCCAGCGCAGGGc  >  1:72637/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGGGTGCCGACGCAGCAGCGGCAGCAAATGCTTGAGCAGCAGAACAGGGGCGAAACAGTTGGTTGTGAAACTGCTCAGCAACCCCGCCATGTCTAGCTGGGTCAGTGCTTTTTCAG‑CCCGCGCCGGGCCTTCTTGCAGCACGCCAAGCGTGCTGAAGACCAGGTTCAGTTGGTCGCAGCAGGCGCCCACCTGCGCGGCCAGTGTTTGCAGTGCCTGCTCGGATCGGGCGTCGCAGTCGAGGCGAACCAACCGTCGCGCGTGCTCTGTGAACAAGGTATCCAGCGCAGGGC  >  NC_002947/3112712‑3113001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑