Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,136,826 G→A 8.9% V10I (GTC→ATC)  PP_2753 → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,136,8260GA 8.9% 125.7 / 2.8 45V10I (GTC→ATC) PP_2753ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/20);  new base A (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGAGCGCGCGCTTTCCAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGC  >  NC_002947/3136679‑3136970
                                                                                                                                                   |                                                                                                                                                
ttttgcgggcgcTTTCCTGCTGCGAATGGGGTTTTGGCCGTAACGGGTAGGGGCAACGCTTTTAAAGGTTTGGGGGCGGCAGGGGCGGCCGGCTTAACTATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGt                                                                                                                                                 <  1:99052/145‑1 (MQ=255)
   aGCGCGCGCTTTCCAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTcag                                                                                                                                              <  5:183874/149‑1 (MQ=255)
       cgcgcTTTCCAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCagcagc                                                                                                                                          <  3:139498/149‑1 (MQ=255)
          gcTTTCCAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGaa                                                                                                                                       >  6:319575/1‑149 (MQ=255)
          gcTTTCCAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGaa                                                                                                                                       >  1:407807/1‑149 (MQ=255)
                cAGTTGCGCCAGGTGCTTTTGCCGGAACCGGTACGTGCAACGAATTTCATCGCTTGGTGGCGGCAGTCGCCGCCGGCTTAACGATGACCAGAGGCTAATAATCCATGACCTATGCGACGCTTCCAGCCGCCGTCAGCAGCGAActgctg                                                                                                                                 <  7:179729/149‑1 (MQ=255)
                cAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAActgctg                                                                                                                                 >  8:46922/1‑149 (MQ=255)
                 aGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTgg                                                                                                                                <  5:319575/149‑1 (MQ=255)
                 aGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTgg                                                                                                                                <  8:100951/149‑1 (MQ=255)
                     gCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCATCAGCAGCGAACTGCTGGCAGt                                                                                                                            >  4:94737/1‑149 (MQ=255)
                     gCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCATCAGCAGCGAACTGCTGGCAGt                                                                                                                            >  4:89178/1‑149 (MQ=255)
                        aCAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTgga                                                                                                                         <  2:407807/149‑1 (MQ=255)
                        aCAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCATCAGCAGCGAACTGCTGGCAGTgga                                                                                                                         <  3:94737/149‑1 (MQ=255)
                        aCAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCATCAGCAGCGAACTGCTGGCAGTgga                                                                                                                         <  3:89178/149‑1 (MQ=255)
                         cAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTggag                                                                                                                        <  1:178355/149‑1 (MQ=255)
                          aGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTggag                                                                                                                        >  8:273007/1‑148 (MQ=255)
                          aGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACAAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTggagg                                                                                                                       <  7:250544/149‑1 (MQ=255)
                            gggCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTggagga                                                                                                                      <  2:293459/148‑1 (MQ=255)
                            gggCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTggagga                                                                                                                      >  5:101897/1‑148 (MQ=255)
                            gggCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTggagga                                                                                                                      >  1:293459/1‑148 (MQ=255)
                              gCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACAt                                                                                                                   <  7:113377/149‑1 (MQ=255)
                              gCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACAt                                                                                                                   >  1:160550/1‑149 (MQ=255)
                                tttGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAAGGATGACCAGAGGGTATTAATCCATGACAATTCCGACGGTACCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATc                                                                                                                  >  8:182725/1‑148 (MQ=255)
                                    gCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAActgctg                                                                                                                                 <  5:367544/129‑1 (MQ=255)
                                    gCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAActgctg                                                                                                                                 >  6:367544/1‑129 (MQ=255)
                                    gCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAg                                                                                                              <  7:46922/148‑1 (MQ=255)
                                          aCCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTa                                                                                                       <  1:401771/149‑1 (MQ=255)
                                                      aaCGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGAt                                                                                           >  5:277795/1‑149 (MQ=255)
                                                           aTTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTg                                                                                      <  6:277795/149‑1 (MQ=255)
                                                           aTTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTg                                                                                      >  7:93639/1‑149 (MQ=255)
                                                                aaGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTg                                                                                 >  3:349757/1‑149 (MQ=255)
                                                                    cTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCg                                                                             <  6:101897/149‑1 (MQ=255)
                                                                                  gcgcCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCgg                                                               <  7:273007/149‑1 (MQ=255)
                                                                                              gAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGAt                                                                                                          <  8:21960/94‑1 (MQ=255)
                                                                                              gAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGAt                                                                                                          >  7:21960/1‑94 (MQ=255)
                                                                                                  gATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGata                                               >  4:113443/1‑149 (MQ=255)
                                                                                                          gagGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGcc                                       <  3:113443/149‑1 (MQ=255)
                                                                                                             ggTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGAAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTg                                    >  4:70701/1‑149 (MQ=255)
                                                                                                                       cATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGcc                           >  8:8470/1‑148 (MQ=255)
                                                                                                                        aTGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCaa                         >  4:58393/1‑149 (MQ=255)
                                                                                                                               aTGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCt                  >  4:147128/1‑149 (MQ=255)
                                                                                                                               aTGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCt                  >  7:176145/1‑149 (MQ=255)
                                                                                                                                      gCTTCCAGCCGTCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCTGGCaa             >  1:51252/1‑147 (MQ=255)
                                                                                                                                      gCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCTGGCaa             >  6:339945/1‑147 (MQ=255)
                                                                                                                                          ccAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCTGGCAAGAGc         <  7:182725/147‑1 (MQ=255)
                                                                                                                                                gccgTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGc  >  1:317169/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTGAGCGCGCGCTTTCCAGCTGCGACAGGGGCTTTGGCCGGAACCGGTACGGGCAACGCATTTCAAGGCTTGGGGGCGGCAGGCGCCGCCCGCTGAACGATGACCAGAGGGTAATAATCCATGACCAATGCGACGCTTCCAGCCGCCGTCAGCAGCGAACTGCTGGCAGTGGAGGACATCGAAGTGATCTACGACGGCACGATCCTTGCCGTGGCCGGTGTATCCCTGCGGGTCGGGCAGGGCGATATCGTCGCCTTGCTAGGCGCCAACGGTGCTGGCAAGAGCACCACGC  >  NC_002947/3136679‑3136970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑