Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,144,909	T→C	10.3%	V106A (GTT→GCT)	PP_2760 →	ribose ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,144,909	0	T	C	10.3%	152.9 / 6.4	58	V106A (GTT→GCT)	PP_2760	ribose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base C (3/3);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGACCGAA  >  NC_002947/3144762‑3145054                                                                                                                                                    |                                                                                                                                                   tCGGCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGAtgtt                                                                                                                                                   >  5:47473/1‑149 (MQ=255) tCGGCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTCGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGAtgtt                                                                                                                                                   >  5:394773/1‑149 (MQ=255)   ggCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGAtgtt                                                                                                                                                   <  1:192885/147‑1 (MQ=255)    gCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTc                                                                                                                                                <  5:10632/149‑1 (MQ=255)    gCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTc                                                                                                                                                <  2:376805/149‑1 (MQ=255)          ttgccttCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATc                                                                                                                                          >  2:1442/1‑149 (MQ=255)             ccttCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGCTGACAACATcgc                                                                                                                                        <  5:214991/148‑1 (MQ=255)                 cGGCCTGCCCACCGCGATCATCGGCGGCGGCTCGAACGTGGGGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCGGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCCTCGCcgcg                                                                                                                                    <  4:345273/148‑1 (MQ=255)                  ggCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGAc                                                                                                                                  >  6:62696/1‑149 (MQ=255)                   gCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGCTGACAACATcgccga                                                                                                                                     <  5:269400/145‑2 (MQ=255)                   gCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGCTGACAACATcgccga                                                                                                                                     >  6:269400/1‑144 (MQ=255)                          cACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCa                                                                                                                          >  7:48140/1‑149 (MQ=255)                             ggTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCActg                                                                                                                       <  1:232384/149‑1 (MQ=255)                                               cTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGc                                                                                                    >  1:406152/1‑149 (MQ=255)                                                          tgtCCGGTGGGCTCGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCtg                                                                                         <  6:394773/149‑1 (MQ=255)                                                            tCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCtggt                                                                                       <  5:211455/149‑1 (MQ=255)                                                               ggTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCtggtggt                                                                                    <  5:140525/149‑1 (MQ=255)                                                                           cTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGttt                                                                           <  7:277791/146‑1 (MQ=255)                                                                           cTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGttt                                                                           >  8:277791/1‑146 (MQ=255)                                                                            tGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGt                                                                       >  7:325190/1‑149 (MQ=255)                                                                              tCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTcc                                                                         <  7:15660/145‑1 (MQ=255)                                                                              tCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGttt                                                                     <  5:62696/149‑1 (MQ=255)                                                                                  tGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTgccg                                                                 <  2:406152/149‑1 (MQ=255)                                                                                      ggcCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCcgccgc                                                             >  7:312731/1‑149 (MQ=255)                                                                                         ctaCCTCGGGTTGTGCGTTGCGGTCTATGCCAGCCTGAACAACGGCGGTATCGGGGATGTTGTCAGCATCGCCGCGACGCTTGGCTCTGGCCTGGCC‑ATCGGCACGCTGATTGGGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCgctgc                                                          <  3:282845/147‑1 (MQ=255)                                                                                           aCCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGctgctg                                                        <  6:405204/149‑1 (MQ=255)                                                                                           aCCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGctgctg                                                        <  6:47473/149‑1 (MQ=255)                                                                                            ccTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTgg                                                       <  1:1442/149‑1 (MQ=255)                                                                                            ccTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTgg                                                       >  6:216041/1‑149 (MQ=255)                                                                                              tcaggTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTAGTCGGTTTCCGTTTGCCGCCGCTGCTGGCg                                                     >  4:23694/4‑149 (MQ=255)                                                                                                    tGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTg                                               >  4:182841/1‑149 (MQ=255)                                                                                                     gTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTg                                               <  4:280555/148‑1 (MQ=255)                                                                                                      tCCGCTGCGGTCTATGCCTGGCTGAACCACGCCGGTTTCTGCGATGTTGTCAGCATCGCCGCGCCCCTTGGCAAGGGCCTGGCCTATCGGCACGCGGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTg                                               <  1:324984/148‑1 (MQ=255)                                                                                                      tCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGc                                             >  7:43351/1‑149 (MQ=255)                                                                                                      tCCGCTGCGGTCTATGCCAACCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGc                                             >  2:33179/1‑149 (MQ=255)                                                                                                        cGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCa                                           >  8:174996/1‑149 (MQ=255)                                                                                                          cTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCAc                                          >  2:403884/1‑148 (MQ=255)                                                                                                             cGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAACATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGAt                                       >  2:134773/1‑148 (MQ=255)                                                                                                                    tGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGcc                                            >  7:125351/1‑136 (MQ=255)                                                                                                                    tGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGcc                                            <  8:125351/136‑1 (MQ=255)                                                                                                                        aGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGc                           <  8:24155/149‑1 (MQ=255)                                                                                                                         gCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGcc                          >  5:3887/1‑149 (MQ=255)                                                                                                                         gCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGcc                          >  5:380237/1‑149 (MQ=255)                                                                                                                          ccTGAACAACGCCGGTTTCGGCGATGCTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCg                         >  1:73715/1‑149 (MQ=255)                                                                                                                            tGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCggg                       >  7:14980/1‑149 (MQ=255)                                                                                                                             gAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGa                                        >  6:203809/1‑131 (MQ=255)                                                                                                                             gAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGa                                        <  5:203809/131‑1 (MQ=255)                                                                                                                                  acGCCGGTTTCGGCGATGCTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGaa                 <  2:73715/149‑1 (MQ=255)                                                                                                                                   cGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCAGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAAc                >  3:297812/1‑149 (MQ=255)                                                                                                                                    gCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACt               >  8:62966/1‑149 (MQ=255)                                                                                                                                     ccGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTg              >  3:36204/1‑149 (MQ=255)                                                                                                                                     ccGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTg              <  8:325190/149‑1 (MQ=255)                                                                                                                                        gTTTCGGCGATCTGCTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTg           <  1:344177/149‑1 (MQ=255)                                                                                                                                             ggCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGAc      <  8:312731/149‑1 (MQ=255)                                                                                                                                             ggCGATGTTGTCAACATCGCCGCGACGCTTGGCACTGGCATGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGa       <  1:134773/148‑1 (MQ=255)                                                                                                                                             ggCGATGCTGACAACATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGAc      >  1:65707/1‑149 (MQ=255)                                                                                                                                              gCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCtggtg                             <  2:164975/125‑1 (MQ=255)                                                                                                                                              gCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCtggtg                             >  1:164975/1‑125 (MQ=255)                                                                                                                                              gCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCggg                       <  3:391798/131‑1 (MQ=255)                                                                                                                                              gCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCggg                       >  4:391798/1‑131 (MQ=255)                                                                                                                                                 aTGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGACCGaa  <  1:403884/149‑1 (MQ=255)                                                                                                                                                   gttgtCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGAc      <  3:324665/143‑1 (MQ=255)                                                                                                                                                   gttgtCAGCATCGCCGCGACGCTTGGAACTCGCCTGACC‑ATCGGCACGCTGAATGCGCTAGTCGTGGTCGGTATCAGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGAAGAACCTGGTGGCCGCGATGGAACTGGTGCTGAc      >  4:324665/1‑143 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCGGCAGCCTTGCCTTCGGCCTGACCACGGTGATCATCGGCGGCGGCTCGAACGTGGTGTCCGGTGGGCTTGATCTGTCGCTGGCGGCCAACCTCGGGTTGTCCGCTGCGGTCTATGCCAGCCTGAACAACGCCGGTTTCGGCGATGTTGTCAGCATCGCCGCGACGCTTGGCACTGGCCTGGCC‑ATCGGCACGCTGAATGCGCTGGTGGTGGTCGGTTTCCGTTTGCCGCCGCTGCTGGCGACCCTGGCCACGATGAACCTGGTGGCCGGGCTGGAACTGGTGCTGACCGAA  >  NC_002947/3144762‑3145054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑