Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,183,329 A→C 6.0% E330A (GAG→GCG)  PP_2793 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,183,3290AC 6.0% 203.2 / 3.4 67E330A (GAG→GCG) PP_2793acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base C (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGAGCCTG  >  NC_002947/3183182‑3183474
                                                                                                                                                   |                                                                                                                                                 
gCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAg                                                                                                                                                  >  4:161224/1‑149 (MQ=255)
  gAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGgc                                                                                                                                                >  8:117198/1‑149 (MQ=255)
         aGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCTCGGCGCtgatc                                                                                                                                         <  5:291059/149‑4 (MQ=255)
              gTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggt                                                                                                                                    <  2:217445/149‑1 (MQ=255)
              gTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggt                                                                                                                                    >  7:339525/1‑149 (MQ=255)
                   tttCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggtggt                                                                                                                                 >  2:226584/1‑147 (MQ=255)
                   tttCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggtggt                                                                                                                                 <  1:226584/147‑1 (MQ=255)
                   tttCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCg                                                                                                                               <  2:274979/149‑1 (MQ=255)
                       aTGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATc                                                                                                                            <  6:123416/148‑1 (MQ=255)
                       aTGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATc                                                                                                                            <  4:177359/148‑1 (MQ=255)
                          cTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGtt                                                                                                                         <  3:324803/148‑1 (MQ=255)
                            ggCCGACACCATGATGGACCTGCACGTGGTGCGTCTGGCGGGCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCg                                                                                                                      <  5:299292/149‑1 (MQ=255)
                               cGACAACATGATGGACCTGAACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCAGGCGCGCCAATGTCGATTCGAGCATGGCCAACGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTAGATCGTTGCGTc                                                                                                                    >  3:217109/1‑148 (MQ=255)
                                gACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCa                                                                                                                  <  4:254579/149‑1 (MQ=255)
                                   aacaTGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCgg                                                                                                                                      <  4:78643/126‑1 (MQ=255)
                                   aacaTGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCgg                                                                                                                                      >  3:78643/1‑126 (MQ=255)
                                   aacaTGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAgg                                                                                                                >  2:297644/1‑148 (MQ=255)
                                           ggACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTg                                                                                                       >  4:320398/1‑149 (MQ=255)
                                            gACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTg                                                                                                       <  1:297644/148‑1 (MQ=255)
                                                 gCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTAGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGAcgcg                                                                                                 >  3:253936/1‑149 (MQ=255)
                                                  cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGCCCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCgg                                                                                                >  1:77230/1‑149 (MQ=255)
                                                  cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCgg                                                                                                <  1:311808/149‑1 (MQ=255)
                                                   aCGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTggg                                                                                                         <  8:373406/139‑1 (MQ=255)
                                                   aCGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTggg                                                                                                         >  7:373406/1‑139 (MQ=255)
                                                     gtggtgCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGt                                                                                             >  8:227648/1‑149 (MQ=255)
                                                      tggtgCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGt                                                                                             >  2:165815/1‑148 (MQ=255)
                                                         tgCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGAcc                                                                                         >  7:320541/1‑149 (MQ=255)
                                                           cGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCggg                                                                                               >  2:322715/1‑141 (MQ=255)
                                                           cGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCggg                                                                                               <  1:322715/141‑1 (MQ=255)
                                                            gTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCggg                                                                                      <  3:320398/149‑1 (MQ=255)
                                                                ggCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGaca                                                                                  >  7:328652/1‑149 (MQ=255)
                                                                 gCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCg                                                                                        >  7:178047/1‑142 (MQ=255)
                                                                 gCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCg                                                                                        <  8:178047/142‑1 (MQ=255)
                                                                 gCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGacac                                                                                 >  2:212388/1‑149 (MQ=255)
                                                                         gCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtggt                                                                           <  7:117198/147‑1 (MQ=255)
                                                                               cgcgTGGGTGCTCGAGCAGGGCGTGCGCGCCAATGTCGATTCTAGCATGGCCAAGGTGATGATCGCCGAGGCGCTGTGGCGGGTGGTCGATGGTTGCGTCCAGGTATTTGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGa                                                                   <  4:217109/149‑1 (MQ=255)
                                                                               cgcCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGa                                                                   <  4:138805/149‑1 (MQ=255)
                                                                                gcCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGAt                                                                  >  3:4407/1‑149 (MQ=255)
                                                                                gcCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGAt                                                                  <  1:409055/149‑1 (MQ=255)
                                                                                 ccTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACAGGGGACACCGTGGTGGAGCGGATc                                                                 >  3:316602/1‑149 (MQ=255)
                                                                                    gggTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTc                                                              >  6:306507/1‑149 (MQ=255)
                                                                                      gTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCcg                                                            <  1:165815/149‑1 (MQ=255)
                                                                                                aGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGc                                                  >  3:92925/1‑149 (MQ=255)
                                                                                                aGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTAGAGCGGATCTTCCGCGACATTCGc                                                  <  2:77230/149‑1 (MQ=255)
                                                                                                  ggCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGccc                                                >  3:329432/1‑149 (MQ=255)
                                                                                                      ggCGTGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTc                                            >  1:194041/1‑149 (MQ=255)
                                                                                                      ggCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGGCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtggtg                                                                          <  4:307282/119‑1 (MQ=255)
                                                                                                           gcCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCAt                                       >  6:342441/1‑149 (MQ=255)
                                                                                                           gcCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCAt                                       >  4:90973/1‑149 (MQ=255)
                                                                                                            acAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATc                                      <  8:339525/148‑1 (MQ=255)
                                                                                                                    gATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACgg                              >  7:279813/1‑149 (MQ=255)
                                                                                                                     aTTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCggg                                                                                      <  1:256679/92‑1 (MQ=255)
                                                                                                                         gAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCg                                          >  8:23228/1‑132 (MQ=255)
                                                                                                                         gAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCg                                          <  7:23228/132‑1 (MQ=255)
                                                                                                                            cATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCg                      <  4:329432/149‑1 (MQ=255)
                                                                                                                              tGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGaa                    <  7:220695/149‑1 (MQ=255)
                                                                                                                              tGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGaa                    <  8:320541/149‑1 (MQ=255)
                                                                                                                                 ccAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGaa                    >  6:134118/1‑146 (MQ=255)
                                                                                                                                 ccAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGaa                    <  5:134118/146‑1 (MQ=255)
                                                                                                                                   aaGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACAGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCa               >  7:36779/1‑149 (MQ=255)
                                                                                                                                     ggTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCAcc             <  1:212388/149‑1 (MQ=255)
                                                                                                                                       gccacAGCGCCTCGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCg            >  3:376099/5‑148 (MQ=255)
                                                                                                                                        ccacAGCGCCTCGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGc                       >  2:188956/4‑136 (MQ=255)
                                                                                                                                        ccacAGCGCCTCGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGc                       <  1:188956/133‑1 (MQ=255)
                                                                                                                                             gcgcCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGc                             <  6:245381/125‑1 (MQ=255)
                                                                                                                                             gcgcCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGc                             >  5:245381/1‑125 (MQ=255)
                                                                                                                                                ccGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGAGCCTg  >  5:343855/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGAGCCTG  >  NC_002947/3183182‑3183474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑