Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,228,103	C→G	6.0%	A314A (GCC→GCG)	PP_2828 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,228,103	0	C	G	6.0%	206.2 / 3.2	67	A314A (GCC→GCG)	PP_2828	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/30);  new base G (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

CGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGT  >  NC_002947/3227956‑3228251                                                                                                                                                    |                                                                                                                                                      cGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGccc                                                                                                                                                      >  2:25179/1‑149 (MQ=255)   cTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGTCGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCg                                                                                                                                                     >  1:286476/1‑148 (MQ=255)      ttGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCa                                                                                                                                                   <  1:244931/147‑1 (MQ=255)          tgaCCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTg                                                                                                                                              >  3:126608/1‑148 (MQ=255)             ccTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑Gc                                                                                                                                         >  6:51879/1‑149 (MQ=255)                gTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGc                                                                                                                                      >  5:239048/1‑149 (MQ=255)                    ccGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGACACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTgg                                                                                                                                   <  1:297782/148‑1 (MQ=255)                     cGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGcc                                                                                                                                 >  1:114786/1‑149 (MQ=255)                      gCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCg                                                                                                                                <  1:25179/149‑1 (MQ=255)                      gCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCg                                                                                                                                >  6:138246/1‑149 (MQ=255)                         cttgcttgAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGgc                                                                                                                             <  5:49545/149‑1 (MQ=255)                          ttgcttgAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGgcg                                                                                                                            >  5:373594/1‑149 (MQ=255)                          ttgcttgAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGgcg                                                                                                                            >  3:356661/1‑149 (MQ=255)                            gcttgAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGgc                                                                                                                             >  5:244804/1‑146 (MQ=255)                            gcttgAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGgc                                                                                                                             <  6:244804/146‑1 (MQ=255)                                 aaGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAgc                                                                                                                      <  4:356661/148‑1 (MQ=255)                                   gagCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCt                                                                                                                   >  3:389294/1‑149 (MQ=255)                                     gcagcagcTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGa                                                                                                                 <  6:239048/149‑1 (MQ=255)                                     gcagcagcTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGa                                                                                                                 <  1:151286/149‑1 (MQ=255)                                      cagcagcTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAg                                                                                                                >  1:56205/1‑149 (MQ=255)                                         cagcTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCt                                                                                                             <  2:56205/149‑1 (MQ=255)                                           gcTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTgg                                                                                                           <  7:15474/149‑1 (MQ=255)                                               gACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTggcagg                                                                                                       >  7:126699/1‑149 (MQ=255)                                               gACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTggca                                                                                                         <  2:286476/147‑1 (MQ=255)                                                  gcgATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGCCTggcaggg                                                                                                      >  4:61172/1‑147 (MQ=255)                                                    gATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGgcagggcagg                                                                                                  <  3:112461/149‑1 (MQ=255)                                                        ctaggTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCggg                                                                                              >  8:322267/4‑149 (MQ=255)                                                         tGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGGACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGt                                                                                             >  3:97567/1‑149 (MQ=255)                                                           ggTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGt                                                                                             <  1:3080/147‑1 (MQ=255)                                                                 cACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGgcagc                                                                                       >  2:212489/1‑147 (MQ=255)                                                                  aCTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCagcaag                                                                                    >  6:267726/1‑149 (MQ=255)                                                                  aCTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCagcaa                                                                                     <  5:138246/148‑1 (MQ=255)                                                                    ttCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCagcaag                                                                                    >  8:200498/1‑147 (MQ=255)                                                                          aCGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGACGCCGCTGGCCGGGCGCCCAGCGCTGAGGCTggcaggg                                                                                                      <  1:72696/124‑1 (MQ=255)                                                                          aCGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACCGCACAGCAGCATGAACTGCGCGCCCCCAGTCTGGA‑GCCGCTGGCCGGGCCCCCAGCGCTCAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGa                                                                            >  4:343580/1‑149 (MQ=255)                                                                          aCGGCTGCGGATGCTTTGCAGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTggcaggg                                                                                                      >  2:72696/1‑123 (MQ=255)                                                                            ggCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGgcagc                                                                                       <  8:107953/136‑1 (MQ=255)                                                                            ggCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGgcagc                                                                                       >  7:107953/1‑136 (MQ=255)                                                                                 cGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACgccgtg                                                                     <  7:208995/149‑1 (MQ=255)                                                                                 cGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACgccgtg                                                                     <  1:267520/149‑1 (MQ=255)                                                                                      gCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGtt                                                                >  6:198877/1‑149 (MQ=255)                                                                                       cTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTg                                                               <  1:25544/149‑1 (MQ=255)                                                                                        tttGCCGCTGTCCGTATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCgg                                                                                               <  5:3934/116‑1 (MQ=255)                                                                                        tttGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCgg                                                                                               >  6:3934/1‑116 (MQ=255)                                                                                         ttGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGc                                                              >  8:144253/1‑148 (MQ=255)                                                                                               cTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTgg                                                       <  5:267726/149‑1 (MQ=255)                                                                                               cTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTgg                                                       <  4:389294/149‑1 (MQ=255)                                                                                                tGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGc                                                      <  2:76906/149‑1 (MQ=255)                                                                                                     gAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGc                                                 >  3:264766/1‑149 (MQ=255)                                                                                                     gAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGc                                                 <  1:212489/149‑1 (MQ=255)                                                                                                           gtcgACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACgg                                           >  6:243659/1‑149 (MQ=255)                                                                                                              gACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCa                                         >  4:215070/1‑148 (MQ=255)                                                                                                               aCTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCAt                                        >  6:145071/1‑148 (MQ=255)                                                                                                                 tCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCTGGTGGCAGCAAGCAGCGTGACGCAGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCaa                                     >  8:234082/1‑149 (MQ=255)                                                                                                                   gCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAAc                                    <  7:144253/148‑1 (MQ=255)                                                                                                                       gCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAgg                                <  3:215070/148‑1 (MQ=255)                                                                                                                          aCGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGccc                             >  4:171804/1‑148 (MQ=255)                                                                                                                               acaGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGAAAGTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTgg                       >  1:134644/1‑149 (MQ=255)                                                                                                                                  gCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGGCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGgtgt                    <  3:343580/149‑1 (MQ=255)                                                                                                                                  gCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGgtgt                    <  4:264766/149‑1 (MQ=255)                                                                                                                                         gAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCctgct             >  1:79135/1‑149 (MQ=255)                                                                                                                                           aCTGCGGGCGCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGct                                         >  2:165066/1‑118 (MQ=255)                                                                                                                                           aCTGCGGGCGCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGct                                         <  1:165066/119‑2 (MQ=255)                                                                                                                                           aCTGCGGGCGCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCa                                         >  2:298307/1‑119 (MQ=255)                                                                                                                                           aCTGCGGGCGCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCa                                         <  1:298307/119‑1 (MQ=255)                                                                                                                                            cTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGtt          <  5:198877/149‑1 (MQ=255)                                                                                                                                               cgcgcCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAg       >  6:62245/1‑149 (MQ=255)                                                                                                                                                gcgcCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGc      <  2:114786/149‑1 (MQ=255)                                                                                                                                                    ccGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGt  >  6:269214/1‑149 (MQ=255)                                                                                                                                                    ccGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGt  >  4:348300/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CGCTATTGATGACCTGTTGCCGCACTTGCTTGAAGAGCAGCAGCTTGACGCGATGCTGGGTTGTCACTTCGAGACGGCTGCGGATGCTTTGCCGCTGTCCGAATTCGTCGACTCGCTAGCCACGGCACAGCTGCATGAACTGCGCGCCCGCAGTCTGGA‑GCCGCTGGCCGGGCGCCCAGCGCTGAGGCTGGCAGGGCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGT  >  NC_002947/3227956‑3228251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑