Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,268,054 C→A 5.7% H175Q (CAC→CAA) ‡ azoR1 → FMN‑dependent NADH‑azoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,268,0540CA 5.7% 228.7 / 3.3 70H175Q (CAC→CAA) ‡azoR1FMN‑dependent NADH‑azoreductase
Reads supporting (aligned to +/- strand):  ref base C (32/34);  new base A (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

AGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGT  >  NC_002947/3267907‑3268201
                                                                                                                                                   |                                                                                                                                                   
aGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACg                                                                                                                                                    >  6:184478/1‑149 (MQ=255)
       tgCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACggcctg                                                                                                                                               <  5:281944/147‑1 (MQ=255)
          ggCAACACGGAACTGGTGCTGGTTCCCACTGCGGGGGGCCTGCACGCTCGCCAGCCGACTGCCGCCGGGCATGAACACTTCCTGAAACTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTa                                                                                                                                          <  8:294168/149‑1 (MQ=255)
                aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                    >  6:339503/1‑149 (MQ=255)
                aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                    >  3:20832/1‑149 (MQ=255)
                aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                    >  8:239059/1‑149 (MQ=255)
                aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                    >  6:145282/1‑149 (MQ=255)
                 cgAAAGGGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGTCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCg                                                                                                                                   <  6:76365/148‑1 (MQ=255)
                   aaaGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCGGa                                                                                                                                 <  2:280781/149‑1 (MQ=255)
                    aaGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGa                                                                                                                                 <  6:129673/148‑1 (MQ=255)
                      gtggtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGagca                                                                                                                              <  4:20832/149‑1 (MQ=255)
                         gtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAgcagcg                                                                                                                           >  3:136457/1‑149 (MQ=255)
                            cTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAgcagcgca                                                                                                                         <  7:239059/148‑1 (MQ=255)
                                 ttCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAgg                                                                                                                   >  6:219353/1‑149 (MQ=255)
                                  tCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAgg                                                                                                                   <  4:231203/148‑1 (MQ=255)
                                     aCTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgat                                                                                                               >  7:64174/1‑149 (MQ=255)
                                     aCTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgat                                                                                                               >  6:308010/1‑149 (MQ=255)
                                          gggTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGANTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtg                                                                                                          >  1:128414/1‑149 (MQ=255)
                                             tGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtgctg                                                                                                       <  8:64174/149‑1 (MQ=255)
                                                ccTGCACGCTGGCCAGCCGACTAGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGca                                                                                                     >  5:335447/1‑148 (MQ=255)
                                                 cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtgctgta                                                                                                     >  7:178436/1‑145 (MQ=255)
                                                 cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGca                                                                                                     >  7:387082/1‑147 (MQ=255)
                                                 cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCGGa                                                                                                                                 >  8:301477/1‑119 (MQ=38)
                                                    cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgc                                                                                                >  8:162782/1‑149 (MQ=255)
                                                    cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgc                                                                                                <  5:145282/149‑1 (MQ=255)
                                                       gCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGAGTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcg                                                                                               >  7:206931/1‑147 (MQ=255)
                                                           gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGTACAGGCGCAGAt                                                                                          <  8:178436/148‑1 (MQ=255)
                                                           gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGAtt                                                                                         <  1:305519/149‑1 (MQ=255)
                                                              aGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGc                                                                                       >  7:97518/1‑148 (MQ=255)
                                                               gCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGc                                                                                       >  2:376991/1‑147 (MQ=255)
                                                                   aCTGGCGCCGGGCATGAAGTCTTCCTGAAAGTGTTCCTGGGCTTCATCGGGTTTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                 <  3:248419/149‑1 (MQ=255)
                                                                   aCTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                 <  3:9260/149‑1 (MQ=255)
                                                                       gcgcCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCt                                                                              <  4:136457/148‑1 (MQ=255)
                                                                               cATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGcaca                                                                                                   >  2:360143/1‑119 (MQ=255)
                                                                               cATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGcaca                                                                                                   <  1:360143/119‑1 (MQ=255)
                                                                                  gAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGc                                                                  >  2:270515/1‑149 (MQ=255)
                                                                                   aaGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGc                                                                  <  2:22767/148‑1 (MQ=255)
                                                                                   aaGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGc                                                                  <  6:335447/148‑1 (MQ=255)
                                                                                      aCTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGACCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGa                                                              >  7:282620/1‑149 (MQ=255)
                                                                                        ttCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGTCCCACGTCCTGGCCTATTGCAAGGAGCAGCGCAGCCAGGCGATCGATTCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGTCTGCATGAt                                                             >  3:342669/1‑148 (MQ=255)
                                                                                        ttCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGAt                                                             >  8:253413/1‑148 (MQ=255)
                                                                                           cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGcc                                                                                                                                     >  8:103731/1‑73 (MQ=255)
                                                                                           cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGcc                                                                                                                                     <  7:103731/73‑1 (MQ=255)
                                                                                           cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCaa                                                          <  5:35905/148‑1 (MQ=255)
                                                                                           cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAg                                                         <  5:219353/149‑1 (MQ=255)
                                                                                            tGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAgg                                                        >  8:359793/1‑149 (MQ=255)
                                                                                            tGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCgg                                                                                                                                  <  6:33037/75‑1 (MQ=21)
                                                                                            tGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCgg                                                                                                                                  >  5:33037/1‑75 (MQ=21)
                                                                                             gAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAgg                                                        >  2:341854/1‑148 (MQ=255)
                                                                                                    ttCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGtt                                                 <  7:162782/148‑1 (MQ=255)
                                                                                                      ccTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTg                                               <  5:308010/148‑1 (MQ=255)
                                                                                                       cTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCg                                                                       <  8:26821/123‑1 (MQ=255)
                                                                                                       cTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCg                                                                       >  7:26821/1‑123 (MQ=255)
                                                                                                       cTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGt                                             <  5:339503/149‑1 (MQ=255)
                                                                                                          ggCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGc                                          <  8:387082/149‑1 (MQ=255)
                                                                                                                aTCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGt                                    >  4:85117/1‑149 (MQ=255)
                                                                                                                      aTTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCt                              >  5:373573/1‑149 (MQ=255)
                                                                                                                         aCTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGt                            <  7:359793/148‑1 (MQ=255)
                                                                                                                          cTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTc                           <  8:97518/148‑1 (MQ=255)
                                                                                                                          cTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTc                           <  3:353252/148‑1 (MQ=255)
                                                                                                                          cTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCg                          <  6:373573/149‑1 (MQ=255)
                                                                                                                             aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                 <  6:322723/91‑1 (MQ=255)
                                                                                                                             aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                 >  5:322723/1‑91 (MQ=255)
                                                                                                                             aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGaaa                       >  3:382874/1‑149 (MQ=255)
                                                                                                                             aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGaaa                       <  5:184478/149‑1 (MQ=255)
                                                                                                                                  gAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAacac                    <  3:85117/147‑1 (MQ=255)
                                                                                                                                            gggCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTcc         >  6:200022/1‑148 (MQ=255)
                                                                                                                                              gCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAAc      <  1:341854/149‑1 (MQ=255)
                                                                                                                                                ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCt    >  2:347562/1‑149 (MQ=255)
                                                                                                                                                  aCGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCACGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGt  <  2:128414/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGT  >  NC_002947/3267907‑3268201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑