Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,268,059 T→A 5.9% L177Q (CTG→CAG)  azoR1 → FMN‑dependent NADH‑azoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,268,0590TA 5.9% 197.7 / 2.5 68L177Q (CTG→CAG) azoR1FMN‑dependent NADH‑azoreductase
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base A (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

TGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAG  >  NC_002947/3267914‑3268206
                                                                                                                                                 |                                                                                                                                                   
tgCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACggcctg                                                                                                                                                    <  5:281944/147‑1 (MQ=255)
   ggCAACACGGAACTGGTGCTGGTTCCCACTGCGGGGGGCCTGCACGCTCGCCAGCCGACTGCCGCCGGGCATGAACACTTCCTGAAACTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTa                                                                                                                                               <  8:294168/149‑1 (MQ=255)
         aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                         >  8:239059/1‑149 (MQ=255)
         aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                         >  6:339503/1‑149 (MQ=255)
         aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                         >  3:20832/1‑149 (MQ=255)
         aaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGccc                                                                                                                                         >  6:145282/1‑149 (MQ=255)
          cgAAAGGGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGTCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCg                                                                                                                                        <  6:76365/148‑1 (MQ=255)
            aaaGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCGGa                                                                                                                                      <  2:280781/149‑1 (MQ=255)
             aaGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGa                                                                                                                                      <  6:129673/148‑1 (MQ=255)
               gtggtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGagca                                                                                                                                   <  4:20832/149‑1 (MQ=255)
                  gtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAgcagcg                                                                                                                                >  3:136457/1‑149 (MQ=255)
                     cTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAgcagcgca                                                                                                                              <  7:239059/148‑1 (MQ=255)
                          ttCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAgg                                                                                                                        >  6:219353/1‑149 (MQ=255)
                           tCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAgg                                                                                                                        <  4:231203/148‑1 (MQ=255)
                              aCTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgat                                                                                                                    >  6:308010/1‑149 (MQ=255)
                              aCTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgat                                                                                                                    >  7:64174/1‑149 (MQ=255)
                                   gggTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGANTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtg                                                                                                               >  1:128414/1‑149 (MQ=255)
                                      tGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtgctg                                                                                                            <  8:64174/149‑1 (MQ=255)
                                         ccTGCACGCTGGCCAGCCGACTAGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGca                                                                                                          >  5:335447/1‑148 (MQ=255)
                                          cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtgctgta                                                                                                          >  7:178436/1‑145 (MQ=255)
                                          cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGca                                                                                                          >  7:387082/1‑147 (MQ=255)
                                          cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCGGa                                                                                                                                      >  8:301477/1‑119 (MQ=38)
                                             cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgc                                                                                                     <  5:145282/149‑1 (MQ=255)
                                             cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgc                                                                                                     >  8:162782/1‑149 (MQ=255)
                                                gCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGAGTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcg                                                                                                    >  7:206931/1‑147 (MQ=255)
                                                    gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGTACAGGCGCAGAt                                                                                               <  8:178436/148‑1 (MQ=255)
                                                    gCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGAtt                                                                                              <  1:305519/149‑1 (MQ=255)
                                                       aGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGc                                                                                            >  7:97518/1‑148 (MQ=255)
                                                        gCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGc                                                                                            >  2:376991/1‑147 (MQ=255)
                                                            aCTGGCGCCGGGCATGAAGTCTTCCTGAAAGTGTTCCTGGGCTTCATCGGGTTTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                      <  3:248419/149‑1 (MQ=255)
                                                            aCTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                      <  3:9260/149‑1 (MQ=255)
                                                                gcgcCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCt                                                                                   <  4:136457/148‑1 (MQ=255)
                                                                        cATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGcaca                                                                                                        >  2:360143/1‑119 (MQ=255)
                                                                        cATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGcaca                                                                                                        <  1:360143/119‑1 (MQ=255)
                                                                           gAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGc                                                                       >  2:270515/1‑149 (MQ=255)
                                                                            aaGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGc                                                                       <  2:22767/148‑1 (MQ=255)
                                                                            aaGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGc                                                                       <  6:335447/148‑1 (MQ=255)
                                                                               aCTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGACCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGa                                                                   >  7:282620/1‑149 (MQ=255)
                                                                                 ttCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGTCCCACGTCCTGGCCTATTGCAAGGAGCAGCGCAGCCAGGCGATCGATTCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGTCTGCATGAt                                                                  >  3:342669/1‑148 (MQ=255)
                                                                                 ttCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGAt                                                                  >  8:253413/1‑148 (MQ=255)
                                                                                    cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGcc                                                                                                                                          <  7:103731/73‑1 (MQ=255)
                                                                                    cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGcc                                                                                                                                          >  8:103731/1‑73 (MQ=255)
                                                                                    cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCaa                                                               <  5:35905/148‑1 (MQ=255)
                                                                                    cTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAg                                                              <  5:219353/149‑1 (MQ=255)
                                                                                     tGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAgg                                                             >  8:359793/1‑149 (MQ=255)
                                                                                     tGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCgg                                                                                                                                       <  6:33037/75‑1 (MQ=21)
                                                                                     tGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCgg                                                                                                                                       >  5:33037/1‑75 (MQ=21)
                                                                                      gAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAgg                                                             >  2:341854/1‑148 (MQ=255)
                                                                                             ttCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGtt                                                      <  7:162782/148‑1 (MQ=255)
                                                                                               ccTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTg                                                    <  5:308010/148‑1 (MQ=255)
                                                                                                cTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCg                                                                            <  8:26821/123‑1 (MQ=255)
                                                                                                cTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCg                                                                            >  7:26821/1‑123 (MQ=255)
                                                                                                cTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGt                                                  <  5:339503/149‑1 (MQ=255)
                                                                                                   ggCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGc                                               <  8:387082/149‑1 (MQ=255)
                                                                                                         aTCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGt                                         >  4:85117/1‑149 (MQ=255)
                                                                                                               aTTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCt                                   >  5:373573/1‑149 (MQ=255)
                                                                                                                  aCTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGt                                 <  7:359793/148‑1 (MQ=255)
                                                                                                                   cTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTc                                <  8:97518/148‑1 (MQ=255)
                                                                                                                   cTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTc                                <  3:353252/148‑1 (MQ=255)
                                                                                                                   cTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCg                               <  6:373573/149‑1 (MQ=255)
                                                                                                                      aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                      <  6:322723/91‑1 (MQ=255)
                                                                                                                      aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGa                                                                                      >  5:322723/1‑91 (MQ=255)
                                                                                                                      aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGaaa                            <  5:184478/149‑1 (MQ=255)
                                                                                                                      aCCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGaaa                            >  3:382874/1‑149 (MQ=255)
                                                                                                                           gAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAacac                         <  3:85117/147‑1 (MQ=255)
                                                                                                                                     gggCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTcc              >  6:200022/1‑148 (MQ=255)
                                                                                                                                       gCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAAc           <  1:341854/149‑1 (MQ=255)
                                                                                                                                         ccACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCt         >  2:347562/1‑149 (MQ=255)
                                                                                                                                           aCGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCACGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGt       <  2:128414/149‑1 (MQ=255)
                                                                                                                                              gcctggcctATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGa    <  1:270515/149‑1 (MQ=255)
                                                                                                                                               cctggcctATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGaa   >  3:65088/1‑149 (MQ=255)
                                                                                                                                               cctggcctATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGaa   <  1:204892/149‑1 (MQ=255)
                                                                                                                                                ctggcctATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAg  >  8:304825/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGATCAAGGGAGCGTTTGGTTGCAAGCGTGGCCCTGTCGAAACACAAGGCCACTCCAACCTGTAGAAG  >  NC_002947/3267914‑3268206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑