Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,273,641 G→C 9.1% G106A (GGC→GCC)  PP_2871 → class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,273,6410GC 9.1% 199.6 / 9.0 66G106A (GGC→GCC) PP_2871class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base C (2/4);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGC  >  NC_002947/3273500‑3273783
                                                                                                                                             |                                                                                                                                                
gCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGcgcg                                                                                                                                           <  2:372459/149‑1 (MQ=255)
 ccTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGCCTGCGCgg                                                                                                                                          <  4:164403/149‑1 (MQ=255)
  cTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGt                                                                                                                                         <  8:78001/149‑1 (MQ=255)
   tGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTg                                                                                                                                        >  6:263626/1‑149 (MQ=255)
     gTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTg                                                                                                                                        <  3:222952/147‑1 (MQ=255)
     gTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGc                                                                                                                                      <  1:354080/149‑1 (MQ=255)
     gTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGCCTGCGCgggtgt                                                                                                                                      <  1:30979/149‑5 (MQ=255)
     gTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGGCAACGCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTg                                                                                                                                        <  3:245008/147‑1 (MQ=255)
       gAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCg                                                                                                                                    >  4:311662/1‑149 (MQ=255)
          gATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCAt                                                                                                                                 >  4:362447/1‑149 (MQ=255)
                 cTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGCCTGCGCgggt                                                                                                                                        <  6:192756/135‑3 (MQ=255)
                 cTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGCCTGCGCgggt                                                                                                                                        >  5:192756/1‑133 (MQ=255)
                   gCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAAtgtg                                                                                                                        <  4:131894/149‑1 (MQ=255)
                   gCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTAAACTCGGCCGGGTTCGACATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGAGCAGGCTGCGCGGTGGCCCCATTGGAAtgtg                                                                                                                        >  2:272402/1‑149 (MQ=255)
                    cAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAAtgtg                                                                                                                        <  7:204654/148‑1 (MQ=255)
                    cAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTgg                                                                                                                       >  1:122589/1‑149 (MQ=255)
                     aGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGc                                                                                                                      <  2:122589/149‑1 (MQ=255)
                     aGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGc                                                                                                                      <  3:318221/149‑1 (MQ=255)
                         gcggcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGTATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCctgc                                                                                                                  >  7:6596/1‑149 (MQ=255)
                            gcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCctg                                                                                                                   >  7:341182/1‑145 (MQ=255)
                            gcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCctg                                                                                                                   <  8:341182/145‑1 (MQ=255)
                            gcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCtgctgc                                                                                                               <  3:362447/149‑1 (MQ=255)
                                     ggCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                       >  2:320716/1‑148 (MQ=255)
                                          ccTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATg                                                                                                >  2:303999/1‑149 (MQ=255)
                                          ccTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATg                                                                                                >  4:387322/1‑149 (MQ=255)
                                              tCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCa                                                                                            >  4:244412/1‑149 (MQ=255)
                                                   cGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAg                                                                                       <  3:311662/149‑1 (MQ=255)
                                                    ggTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCa                                                                                            >  6:160428/1‑143 (MQ=255)
                                                    ggTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCa                                                                                            <  5:160428/143‑1 (MQ=255)
                                                    ggTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGt                                                                                      >  3:387649/1‑149 (MQ=255)
                                                     gTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGtt                                                                                     >  1:329143/1‑149 (MQ=255)
                                                          cccGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACg                                                                                <  5:263626/149‑1 (MQ=255)
                                                           ccggccggGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACgg                                                                               <  2:329143/149‑1 (MQ=255)
                                                               ccggGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAAt                                                                           <  6:203535/149‑1 (MQ=255)
                                                                    ttCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGGGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACg                                                                                <  7:143531/139‑1 (MQ=255)
                                                                    ttCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACg                                                                                >  8:143531/1‑139 (MQ=255)
                                                                        tCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTAc                                                                  >  6:306728/1‑149 (MQ=255)
                                                                          aTTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTAcc                                                                 <  4:13459/148‑1 (MQ=255)
                                                                           ttCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACACACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGATTTACGGCAATGTGGCTTACCAc                                                               >  5:333183/1‑149 (MQ=255)
                                                                                 gTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAt                                                         <  1:188276/149‑1 (MQ=255)
                                                                                    ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAg                                                     <  5:306728/149‑1 (MQ=255)
                                                                                     cATTCACGCTGCCCGCGAGTATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAgg                                                    <  8:6596/149‑1 (MQ=255)
                                                                                      aTTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAggg                                                   >  1:89648/1‑149 (MQ=255)
                                                                                       ttCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGc                                                  >  5:62644/1‑149 (MQ=255)
                                                                                       ttCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGc                                                  >  6:172063/1‑149 (MQ=255)
                                                                                              tGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCgcgc                                           >  4:23799/1‑149 (MQ=255)
                                                                                                  cgcgAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGa                                       <  4:387649/149‑1 (MQ=255)
                                                                                                  cgcgAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGAGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGa                                       >  4:237139/1‑149 (MQ=255)
                                                                                                     gAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGTTTGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACa                                     <  1:320716/149‑1 (MQ=255)
                                                                                                     gAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACAt                                    <  7:318602/149‑1 (MQ=255)
                                                                                                        gATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGa                                 <  1:303999/149‑1 (MQ=255)
                                                                                                           gCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATg                               >  2:220310/1‑148 (MQ=255)
                                                                                                             ccGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGaa                             >  8:34865/1‑148 (MQ=255)
                                                                                                                       ctgcAAACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCTGTCAACCAG‑ATTTCCATTGAGTTTTTCGGCAATGTGGCTTACCACGCCTATGGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGc                   <  1:272402/149‑1 (MQ=255)
                                                                                                                         gcACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtgg                >  4:385075/1‑149 (MQ=255)
                                                                                                                         gcACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtgg                >  1:297983/1‑149 (MQ=255)
                                                                                                                          cacacccacaccCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtggt               >  5:139320/1‑149 (MQ=255)
                                                                                                                          cacacccacaccCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtggt               >  2:284547/1‑149 (MQ=255)
                                                                                                                             acccacacccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCATCCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGc            >  4:243376/1‑149 (MQ=255)
                                                                                                                                 gccaccGCGCAGCCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACAGGTATGAACAGCAAAGGCtggt               >  6:15621/5‑142 (MQ=255)
                                                                                                                                 gccaccGCGCAGCCTGCGCGGTGGCAGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtggt               <  5:15621/138‑1 (MQ=255)
                                                                                                                                   acccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGa                              >  7:121233/1‑125 (MQ=255)
                                                                                                                                   acccGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGa                              <  8:121233/125‑1 (MQ=255)
                                                                                                                                     ccGCGCAGGCTGCGCGGTGGCCAAATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGAc        >  7:255045/1‑145 (MQ=255)
                                                                                                                                     ccGCGCAGGCGGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGAc        <  8:255045/145‑1 (MQ=255)
                                                                                                                                       gcgcAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGGCCATGGAGGTTTACGGCAATCTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGTCTGGTGGCCGACCTGGGc  <  3:237139/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GCCTGGTGAAGATCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGGCCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAG‑ATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTAT‑GAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGC  >  NC_002947/3273500‑3273783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑