Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,324,435 G→A 9.0% G233G (GGC→GGT)  PP_2924 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,324,4350GA 9.0% 155.5 / 2.6 55G233G (GGC→GGT) PP_2924hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (3/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GCGCAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCT  >  NC_002947/3324288‑3324581
                                                                                                                                                   |                                                                                                                                                   
gcgcAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGTGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGgcg                                                                                                                                                     <  2:96992/148‑1 (MQ=255)
gcgcAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGgcgc                                                                                                                                                    <  6:278471/149‑1 (MQ=255)
gcgcAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGgcgc                                                                                                                                                    >  2:157772/1‑149 (MQ=255)
gcgcAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGgcgc                                                                                                                                                    >  1:233729/1‑149 (MQ=255)
gcgcAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGgcgc                                                                                                                                                    <  7:170183/149‑1 (MQ=255)
   cagcagCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAATGTACTGTCGTCGGCGCCGACAGCACcga                                                                                                                                                 <  7:78767/149‑1 (MQ=255)
       agcGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCAATGCCCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCGGACGAt                                                                                                                                             >  8:151244/1‑149 (MQ=255)
         cGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGa                                                                                                                                           <  3:400786/149‑1 (MQ=255)
              gtgCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTgg                                                                                                                                      >  6:156327/1‑149 (MQ=255)
                 cggcgAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTg                                                                                                                                   <  5:285666/149‑1 (MQ=255)
                    cgAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGctc                                                                                                                                <  5:300202/149‑1 (MQ=255)
                        gggTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTACTACACGAGCTTGAGGCCGTAGCCCTCGTACAGCCACTCGGCATTGAGCACCAACGTACTGTCGTCGGTCCTGTCGGCGCCGACGATGACGTGGTTCCTCTGca                                                                                                                            >  6:220834/1‑148 (MQ=255)
                         ggTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGccc                                                                                                                           <  2:233729/149‑1 (MQ=255)
                           tACGCTGTCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGAAGTACACGCGCTTGAGGCCGTAGCCCTAGTACAGCGACTCGGCATTGCGAAGCAAGGTACTGTCCTCGGAGCTGTCGGCGACGACGATCACATGGGTGCTCTGCccgg                                                                                                                         >  5:22146/1‑149 (MQ=255)
                              gCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCTGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCccggccg                                                                                                                      >  7:262013/1‑149 (MQ=255)
                                tGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCcga                                                                                                                                                 <  5:22995/120‑1 (MQ=255)
                                tGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCcga                                                                                                                                                 >  6:22995/1‑120 (MQ=255)
                                  ccGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGt                                                                                                                  <  1:172954/149‑1 (MQ=255)
                                   cGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGGTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTa                                                                                                                 <  3:201640/149‑1 (MQ=255)
                                               ggATCGGGCTGAAGGGAGAGTTGTACAGGCGCTTGAGCCCGTTGCCCTGGAACAGCGACTCGGCATTGCGCAGCATGGTACTGTCGTCGGTGCGTTCGGGGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGcc                                                                                                     <  5:220834/149‑1 (MQ=255)
                                                    gggCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTgg                                                                                                 <  5:155545/148‑1 (MQ=255)
                                                      gCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCt                                                                                              >  1:177145/1‑149 (MQ=255)
                                                       cTGAAGGCAGCGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCGGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTc                                                                                             <  7:387409/149‑1 (MQ=255)
                                                        tGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCg                                                                                            >  3:52405/1‑149 (MQ=255)
                                                             gCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTg                                                                                         <  8:263518/147‑1 (MQ=255)
                                                             gCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGCTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTg                                                                                         >  7:263518/1‑147 (MQ=255)
                                                                 agtagtACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGc                                                                                               >  3:71132/1‑137 (MQ=255)
                                                                 agtagtACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGc                                                                                               <  4:71132/137‑1 (MQ=255)
                                                                    agtaCACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCATGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑Gc                                                                                <  3:295855/148‑1 (MQ=255)
                                                                      taCACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCctg                                                                             >  1:142998/1‑149 (MQ=255)
                                                                            cgcTTGAGGCCGGAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGcc                                                                       <  6:374546/149‑1 (MQ=255)
                                                                              cTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCt                                                                     <  3:242650/149‑1 (MQ=255)
                                                                               ttGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCt                                                                     >  2:294162/1‑148 (MQ=255)
                                                                                     ccGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAAt                                                              <  1:157772/149‑1 (MQ=255)
                                                                                          gCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGcc                                                                       >  7:49041/1‑135 (MQ=255)
                                                                                          gCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGcc                                                                       <  8:49041/135‑1 (MQ=255)
                                                                                           cccTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGcc                                                        >  7:12076/1‑149 (MQ=255)
                                                                                             cTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTTCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCa                                                      >  1:288516/1‑149 (MQ=255)
                                                                                              tGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGACGGTGCTGTCGGCGACGACGATGACTTGGGTGCTCTGCCCGGCCGCGGTAAAGCGCGGCGCCCTGGGCTCGTAGTCCACG‑CCCTGCTGGCCCTGATGAATCACGCCCAt                                                     >  4:68082/1‑149 (MQ=255)
                                                                                                 tACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGc                                                  <  2:263088/149‑1 (MQ=255)
                                                                                                  aCAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGcc                                                 >  8:111657/1‑149 (MQ=255)
                                                                                                   cAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCt                                                <  2:211060/149‑1 (MQ=255)
                                                                                                       gACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGc                                             >  5:197089/1‑148 (MQ=255)
                                                                                                          tCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAAt                                         >  4:243607/1‑149 (MQ=255)
                                                                                                            ggCATTGCGCAGCAATGTACCGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATg                                        <  8:194381/148‑1 (MQ=255)
                                                                                                               aTTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCctg                                                                             <  1:117349/108‑1 (MQ=255)
                                                                                                               aTTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCctg                                                                             >  2:117349/1‑108 (MQ=255)
                                                                                                                  gcgcAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGCGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGtgtg                                   <  1:294162/148‑1 (MQ=255)
                                                                                                                   cgcAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGGCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGccc                                >  3:94230/1‑149 (MQ=255)
                                                                                                                                catcgtcgGCGCCGACAGCACCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGgc                   >  1:227496/3‑149 (MQ=255)
                                                                                                                                  tcgtcgGCGCCGACAGCACCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGgcgc                 >  1:215966/1‑149 (MQ=255)
                                                                                                                                   cgtcgGCGCCGACAGCACCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGc                                                         <  2:333747/108‑1 (MQ=255)
                                                                                                                                   cgtcgGCGCCGACAGCACCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCAGATGAATCACGc                                                         >  1:333747/1‑108 (MQ=255)
                                                                                                                                        gTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGgcg           >  5:232161/1‑149 (MQ=255)
                                                                                                                                            tGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCtt        >  1:405463/1‑148 (MQ=255)
                                                                                                                                              tCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCtt        <  2:405463/146‑1 (MQ=255)
                                                                                                                                              tCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTg       <  2:288516/147‑1 (MQ=255)
                                                                                                                                              tCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTGAg     >  6:330020/1‑149 (MQ=255)
                                                                                                                                              tCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTGAg     >  2:181742/1‑149 (MQ=255)
                                                                                                                                              tCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTGAg     >  6:215023/1‑149 (MQ=255)
                                                                                                                                                 gcgcCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCCGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCt  <  2:142998/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGCAGCAGCGGTGGTGCGGCGAGGGGTACGCTGCCGGGGCTGTCGGGGATCGGGCTGAAGGCAGAGTAGTACACGCGCTTGAGGCCGTAGCCCTGGTACAGCGACTCGGCATTGCGCAGCAAGGTACTGTCGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACG‑GCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGTGCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCT  >  NC_002947/3324288‑3324581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑