Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	262,942	A→C	6.8%	intergenic (+177/‑308)	PP_0211 → / → gabD‑I	hypothetical protein/succinate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	262,942	0	A	C	6.8%	222.1 / 7.7	74	intergenic (+177/‑308)	PP_0211/gabD‑I	hypothetical protein/succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (41/28);  new base C (3/2);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACA  >  NC_002947/262797‑263089                                                                                                                                                  |                                                                                                                                                     aGAAGCAGGAAGACCTCCTAGCCAACGATCTGGCAGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGAACGGCAt                                                                                                                                                     >  7:184601/1‑147 (MQ=255)   aaGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAgg                                                                                                                                                 >  1:222484/1‑149 (MQ=255)     gCAGGACGAAAGCCTAGACAACGAACTGGCCGACGCTAACCTGATCATTGGCGACACCAAGAGTGTGGGTACAGGGCGCAACTGCGCAAAGTTCCACCGATTACGATGCCGATGAAAACAGCATCATCATTTCAGCACCGCAACAgtcc                                                                                                                                               >  6:393897/1‑146 (MQ=255)       aGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGt                                                                                                                                             >  6:136401/1‑149 (MQ=255)         gacgacCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑gc                                                                                                                                          <  1:155380/149‑1 (MQ=255)                        aaCGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑gct                                                                                                                                         <  2:66032/135‑1 (MQ=255)                        aaCGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑gct                                                                                                                                         >  1:66032/1‑135 (MQ=255)                           gAACTGGCCGAGGGTAACCTGCTCTTTGGAGACACACCGAGTGTGGGTACAGGGCGCAACTGCGCAACGAGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCACCACGGCATCAGGGCGT‑GATGAGATGATCGACCTCGc                                                                                                                        >  8:189149/1‑149 (MQ=255)                             aCTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGcc                                                                                                                       >  1:117187/1‑148 (MQ=255)                              cTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GTTGAGCTGATCGACCTCGCCaa                                                                                                                     <  6:53909/149‑1 (MQ=255)                                   cGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGt                                                                                                                >  7:146006/1‑149 (MQ=255)                                    gAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGtt                                                                                                               <  5:136401/149‑1 (MQ=255)                                      gggTTACCTGCTCTTTGGCGACACCCCTAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCt                                                                                                             <  7:189149/149‑1 (MQ=255)                                        gTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGa                                                                                                           >  3:326161/1‑149 (MQ=255)                                         tAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGGATCATCGTTTCAGCACGGCATCAGGGCGTAGCTGAGCTGATCGACCTCGCCAAGGCGTTCTGa                                                                                                           <  1:254797/149‑1 (MQ=255)                                           aCCTGCTCTTTGGAGACACCCCGATTGTGGGTACTCTGCGCAACTTGGCAACTTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCATCACGGCATCAGGGCTT‑GCTTAGCTGATCGACCTCGCCAAGGCGTTCTGAtt                                                                                                         <  5:393897/148‑1 (MQ=255)                                            ccTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑gctgagctga                                                                                                                                  >  5:121160/1‑122 (MQ=255)                                            ccTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑gctgagctga                                                                                                                                  <  6:121160/122‑1 (MQ=255)                                             cTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCAt                                                                                                      <  8:273231/149‑1 (MQ=255)                                                   tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATccc                                                                                                   <  4:326161/146‑1 (MQ=255)                                                   tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                                >  6:390227/1‑149 (MQ=255)                                                   tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                                >  5:210152/1‑149 (MQ=255)                                                   tttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTc                                                                                                >  8:12788/1‑149 (MQ=255)                                                    ttGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTcc                                                                                               >  6:116340/1‑149 (MQ=255)                                                     tGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCg                                                                                              >  8:273009/1‑149 (MQ=255)                                                      ggCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGTATCATCCTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCg                                                                                              <  7:21639/148‑1 (MQ=255)                                                      ggCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCgg                                                                                             >  5:294089/1‑149 (MQ=255)                                                           cacCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAggg                                                                                        <  7:1250/149‑1 (MQ=255)                                                             ccccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGcc                                                                                      >  8:31370/1‑149 (MQ=255)                                                              cccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCg                                                                                     >  8:221950/1‑149 (MQ=255)                                                              cccGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCg                                                                                     >  8:216068/1‑149 (MQ=255)                                                               ccGAGTGTGGGTACAGGGCGCAACTGGTCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGCGAGT‑GCTGAGCTGATCGACATCGCCAAGGCTATCACATTCATCCCCTCCGGGAGGGACGt                                                                                    >  3:13650/1‑149 (MQ=255)                                                                cGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTc                                                                                   >  2:32682/1‑149 (MQ=255)                                                                        ggTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTccccc                                                                           >  1:350733/1‑149 (MQ=255)                                                                         gTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTcccccc                                                                          >  6:98753/1‑149 (MQ=255)                                                                             aGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTca                                                                      >  3:61904/1‑149 (MQ=255)                                                                              gggCCCACCCGGGCAACGTGCCACCGAATCCGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCAGCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTcac                                                                     <  6:387305/149‑1 (MQ=255)                                                                               ggCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTACGGGAGGGCCGTCGGTCCCCCCTTcaca                                                                    >  4:402837/1‑149 (MQ=255)                                                                                    aaCTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttct                                                               >  2:261508/1‑149 (MQ=255)                                                                                      cTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCCCCGGGAGGGCCGTCGGTCCCCCCTTCACAtttcttt                                                             <  8:146006/149‑1 (MQ=255)                                                                                       tGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTg                                                                                                            <  7:320862/101‑1 (MQ=255)                                                                                       tGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTg                                                                                                            >  8:320862/1‑101 (MQ=255)                                                                                       tGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGCCGT‑CCTCAGCTGATCTACCTCGCCAAGGCGTTCTCATTCATCACCTCCGGGACGGCCGTCCGTCCCACCATAACAtttcttt                                                             >  7:317771/1‑148 (MQ=255)                                                                                         ggCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCa                                                           <  5:98753/148‑1 (MQ=255)                                                                                         ggCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCa                                                           <  4:61904/148‑1 (MQ=255)                                                                                          gCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGt                                                         <  2:198995/149‑1 (MQ=255)                                                                                           cAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCATCGGTCCCCCCTTCACATTTCTTTCAGtt                                                        >  8:299076/1‑149 (MQ=255)                                                                                                tGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTc                                                   <  7:273009/149‑1 (MQ=255)                                                                                                 gCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCg                                                  <  2:350733/149‑1 (MQ=255)                                                                                                   cACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                >  6:352205/1‑149 (MQ=255)                                                                                                    aCCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTc                                                >  1:353767/1‑148 (MQ=255)                                                                                                       gATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCGTTCACATTTCTTTCAGTTCATTCGTCTAAc                                            <  1:32682/149‑1 (MQ=255)                                                                                                        acgatgATGCTGTTGTCATCGGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAcc                                           >  4:245317/6‑149 (MQ=255)                                                                                                            cGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTa                                       <  7:12788/149‑1 (MQ=255)                                                                                                             gATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTAc                                      >  8:18581/1‑149 (MQ=255)                                                                                                                  cGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGcc                                 <  2:222484/149‑1 (MQ=255)                                                                                                                       acaacaGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATc                            >  4:136702/1‑149 (MQ=255)                                                                                                                        caacaGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCg                           >  5:250461/1‑149 (MQ=255)                                                                                                                          tcggCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCt                         <  3:245317/146‑1 (MQ=255)                                                                                                                                tcatcGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAg                   >  3:193466/1‑149 (MQ=255)                                                                                                                                     gTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGc              >  8:53566/1‑149 (MQ=255)                                                                                                                                      tttCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGGCAGCt             >  8:252720/1‑149 (MQ=255)                                                                                                                                      tttCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCt             >  4:251014/1‑149 (MQ=255)                                                                                                                                      tttCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCATCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCt             <  7:299076/149‑1 (MQ=255)                                                                                                                                      gctCAGCACGCCCTGATGCCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTc                                                   <  2:333963/109‑1 (MQ=255)                                                                                                                                      gctCAGCACGCCCTGATGCCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTc                                                   >  1:333963/3‑111 (MQ=255)                                                                                                                                       ctCAGCACGCCCTGATGCCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTc            >  4:94911/2‑149 (MQ=255)                                                                                                                                        tCAGCACGCCCTGATGCCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                            >  3:172291/1‑116 (MQ=255)                                                                                                                                        tCAGCACGCCCTGATGCCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAAc                                            <  4:172291/116‑1 (MQ=255)                                                                                                                                            cACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCGATGGTGTTCTGATTCATCCCCTGCGGGAGGGGCGTCGGTCAGGCCTTCTCATTTCTTTGAGTTCATTCGTCTTACCTGTTCTTGGCCTATTTCTGGCAAGCCAGCttcgac        <  4:13650/148‑6 (MQ=255)                                                                                                                                            cACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcac        >  5:355100/1‑148 (MQ=255)                                                                                                                                             aCGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTTGCCCTATCGCTGGCAAGCCAGCTCCaaca       >  3:256403/1‑148 (MQ=255)                                                                                                                                             aCGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcacaa      <  1:261508/149‑1 (MQ=255)                                                                                                                                               ggCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcacaaca    <  6:210152/149‑1 (MQ=255)                                                                                                                                                  aTCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCcacaacaca  >  8:151539/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                     AGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGCCACCGATTACGATGCCGATGACAACAGCATCATCGTTTCAGCACGGCATCAGGGCGT‑GCTGAGCTGATCGACCTCGCCAAGGCGTTCTGATTCATCCCCTCCGGGAGGGCCGTCGGTCCCCCCTTCACATTTCTTTCAGTTCATTCGTCTAACCTGTACTGGCCCTATCGCTGGCAAGCCAGCTCCCACAACACA  >  NC_002947/262797‑263089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑