Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,409,918 G→T 7.7% intergenic (‑484/‑52) PP_3022 ← / → PP_3024 AraC family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,409,9180GT 7.7% 178.3 / 4.2 60intergenic (‑484/‑52)PP_3022/PP_3024AraC family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/23);  new base T (2/3);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

ATACCTATTCCTGGCCATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCATTGAATGGATA  >  NC_002947/3409771‑3410064
                                                                                                                                                   |                                                                                                                                                   
aTACCTATTCCTGGCCATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTgg                                                                                                                                                    >  8:114372/1‑149 (MQ=255)
   ccTATTCCTGGCCATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTggcgg                                                                                                                                                 >  1:225991/1‑149 (MQ=255)
    cTATTCCTGGCCATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTggcggc                                                                                                                                                >  1:346915/1‑149 (MQ=255)
            ggCCATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAAcc                                                                                                                                        >  3:239325/1‑149 (MQ=255)
              ccATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAAccc                                                                                                                                       >  3:315850/1‑148 (MQ=255)
                 tCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGc                                                                                                                                   <  1:263311/149‑1 (MQ=255)
                   aaGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGa                                                                                                                                 >  4:338285/1‑149 (MQ=255)
                               gcgGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTTGCCGGCCGCCAcccag                                                                                                                                     <  6:98537/133‑3 (MQ=255)
                               gcgGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTTGCCGGCCGCCAcccag                                                                                                                                     >  5:98537/1‑131 (MQ=255)
                                 ggCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAAc                                                                                                                   <  4:239325/149‑1 (MQ=255)
                                     tACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTa                                                                                                               >  3:230928/1‑149 (MQ=255)
                                       ccTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAg                                                                                                              <  7:114372/148‑1 (MQ=255)
                                             cTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAg                                                                                                       <  3:338285/148‑1 (MQ=255)
                                              tACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAggg                                                                                                     >  3:204486/1‑149 (MQ=255)
                                               aCATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAggg                                                                                                     >  4:181512/1‑148 (MQ=255)
                                                cATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCACCCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGt                                                                                                    >  7:217750/1‑148 (MQ=255)
                                                 aTCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTa                                                                                                   >  4:114417/1‑148 (MQ=255)
                                                  tCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTTGCCGGCCGCCAccca                                                                                                                                      <  2:308011/113‑2 (MQ=255)
                                                  tCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTTGCCGGCCGCCAccca                                                                                                                                      >  1:308011/1‑112 (MQ=255)
                                                     ccTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTg                                                                                              <  2:225991/149‑1 (MQ=255)
                                                     ccTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTg                                                                                              >  1:249728/1‑149 (MQ=255)
                                                                  gcgcgcACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTc                                                                                  <  5:135664/148‑1 (MQ=255)
                                                                          ccGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGc                                                                             <  7:314388/145‑1 (MQ=255)
                                                                          ccGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGc                                                                             >  8:314388/1‑145 (MQ=255)
                                                                          ccGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCAtt                                                                          >  1:155054/1‑148 (MQ=255)
                                                                            gTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGAc                                                                       <  2:249728/149‑1 (MQ=255)
                                                                            gTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGAc                                                                       >  4:175796/1‑149 (MQ=255)
                                                                             tGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACt                                                                      >  2:130749/1‑149 (MQ=255)
                                                                             tGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACt                                                                      <  8:147669/149‑1 (MQ=255)
                                                                               aGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTc                                                                     <  2:346915/148‑1 (MQ=255)
                                                                                 cGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTcagc                                                                  <  4:204486/149‑1 (MQ=255)
                                                                                   agaCCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTcagcag                                                                <  3:175796/149‑1 (MQ=255)
                                                                                     aCCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAgg                                                               <  4:230928/148‑1 (MQ=255)
                                                                                     aCCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGc                                                              >  8:268422/1‑149 (MQ=255)
                                                                                        gtggtgGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGaa                                                           >  6:322749/1‑149 (MQ=255)
                                                                                            tggCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGaa                                                           <  6:7852/145‑1 (MQ=255)
                                                                                            tggCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGaa                                                           >  5:7852/1‑145 (MQ=255)
                                                                                             ggCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACagag                                                      >  2:22937/1‑149 (MQ=255)
                                                                                              gCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCCACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAgg                                                     >  5:393064/1‑149 (MQ=255)
                                                                                              gCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAgg                                                     >  5:64767/1‑149 (MQ=255)
                                                                                              gCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAgg                                                     >  1:208116/1‑149 (MQ=255)
                                                                                              gCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAgg                                                     >  2:275088/1‑149 (MQ=255)
                                                                                                aaGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCCTCCATTGACTCAGCATGCGAACAGAGGc                                                    >  8:384243/1‑148 (MQ=255)
                                                                                                   cGATCACCCAGGCGTCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAAt                                                <  6:64767/149‑1 (MQ=255)
                                                                                                         gccAGGCGCCAAGTGCCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCAc                                          >  7:76303/2‑149 (MQ=255)
                                                                                                          ccAGGCGGCACTTGGCGCCTGGCGCGACAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACg                                         >  2:118693/1‑149 (MQ=255)
                                                                                                           ctggcggcCCTTTGCGCCTGGCGCGCAAGGAGTTCTGGGTTGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGc                                        <  4:315850/147‑1 (MQ=255)
                                                                                                                ggcACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTc                                   >  7:104516/1‑149 (MQ=255)
                                                                                                                       ggCGCCTGGCTCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTcgtacgta                            >  4:21279/1‑149 (MQ=255)
                                                                                                                       ggCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACt                              <  3:114417/147‑1 (MQ=255)
                                                                                                                        gcgcCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTa                            >  1:194223/1‑148 (MQ=255)
                                                                                                                               gcgcgcCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCt                                                                                   >  7:6337/1‑86 (MQ=255)
                                                                                                                               gcgcgcCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCt                                                                                   <  8:6337/86‑1 (MQ=255)
                                                                                                                               gcgcgcCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATc                      <  2:155054/147‑1 (MQ=255)
                                                                                                                               gcgcgcCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCa                     <  6:393064/148‑1 (MQ=255)
                                                                                                                                        gAGTTCTGGGTGGCGGCCGGCACCCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCCCGCTGGTCGTACTTTCAAATCACCTGCGCagt           >  4:283578/1‑147 (MQ=255)
                                                                                                                                        gAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCAtt           <  1:22937/149‑1 (MQ=255)
                                                                                                                                              tGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCTTGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCATTGAATgg     <  7:384243/149‑1 (MQ=255)
                                                                                                                                              tGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCATTGAATg      >  4:350340/1‑148 (MQ=255)
                                                                                                                                                ggTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGCATTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCACATTGAAt       >  1:82905/1‑146 (MQ=255)
                                                                                                                                                ggTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCACATTGAAt       <  2:82905/145‑1 (MQ=255)
                                                                                                                                                ggTGGCGGCAGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCATTGAATGGat   <  8:104516/149‑1 (MQ=255)
                                                                                                                                                  tGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCATTGAATGGata  >  2:133317/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATACCTATTCCTGGCCATCAAGGTGGTGGGCGCGGCCTACCTGTTCTACATCGCCTGGCAACTGTGGCGCGCACCCGTGAGCGAGACCGTGGTGGCAAGCGATCACCCAGGCGGCACTTGGCGCCTGGCGCGCCAGGAGTTCTGGGTGGCGGCCGGCAACCCTAGCGACTGACAATATAAACTCTAGC‑TTCAGGGTAAATTGGTATCAACCCTCCATGCATTGACTCAGCAGGCGAACAGAGGCAAATTGCCACGCTTGTCGTACTTACAAATCACCTGCGCATTGAATGGATA  >  NC_002947/3409771‑3410064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑