Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,455,168 G→A 7.8% N241N (AAC→AAT)  aacs ← acetoacetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,455,1680GA 7.8% 145.5 / 2.2 51N241N (AAC→AAT) aacsacetoacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

AACAGCACGCCTCCGGCGCGATGGACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGCATACTGGTAACCGGC  >  NC_002947/3455022‑3455311
                                                                                                                                                  |                                                                                                                                               
aagaGCACGCCTCCGGCGCGATGGACATTCCAGTTGCGCACGCCGGTGGTGCCACTGGAATACAGGATCCACAGCGGGTGGGCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCGTGAAGAAGTCATTCCACAGGCTGACGtt                                                                                                                                               <  6:297885/146‑1 (MQ=255)
aaCAGCACGCCTCCGGCGCGATGGACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGtt                                                                                                                                               >  1:321192/1‑149 (MQ=255)
        gCCTCCGGCGCGATGGACAATGCACTTGGGCACGCCGGTGGTACCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCt                                                                                                                                       >  8:51802/1‑149 (MQ=255)
             cGGCGCGATGGACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGaa                                                                                                                                   <  3:203361/148‑1 (MQ=255)
                 gcgATGGACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCa                                                                                                                               >  8:188048/1‑148 (MQ=255)
                      ggACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCa                                                                                                                         >  1:149169/1‑149 (MQ=255)
                       gACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCa                                                                                                                         <  4:218829/148‑1 (MQ=255)
                       gACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCAc                                                                                                                        <  2:168610/149‑1 (MQ=255)
                        aCAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGATCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCAcg                                                                                                                       >  6:241855/1‑149 (MQ=255)
                        aCAATGCACTTGGGCACGCCGGGGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCAcg                                                                                                                       <  5:328772/149‑1 (MQ=255)
                         cAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCAcgc                                                                                                                      <  2:149169/149‑1 (MQ=255)
                          aaTGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCAcgcg                                                                                                                     >  3:263543/1‑149 (MQ=255)
                          aaTGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCAcgcg                                                                                                                     >  3:386501/1‑149 (MQ=255)
                              cACTTGGGCACGCCGGTGGTACCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGc                                                                                                                  <  7:51802/148‑1 (MQ=255)
                                        cGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTa                                                                                                       >  6:316605/1‑149 (MQ=255)
                                           cGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATgg                                                                                                    <  3:5484/149‑1 (MQ=255)
                                                  gCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCg                                                                                                           >  6:113286/1‑135 (MQ=255)
                                                  gCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCg                                                                                                           <  5:113286/135‑1 (MQ=255)
                                                          aaTAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATgg                                                                                                    <  2:54484/134‑1 (MQ=255)
                                                          aaTAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATgg                                                                                                    >  1:54484/1‑134 (MQ=255)
                                                               agGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTc                                                                                <  4:386501/149‑1 (MQ=255)
                                                                ggATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTcc                                                                               <  3:317076/149‑1 (MQ=255)
                                                                 gATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTcca                                                                              <  4:263543/149‑1 (MQ=255)
                                                                  aTGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTccag                                                                             >  8:163564/1‑149 (MQ=255)
                                                                       cAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTccaggccag                                                                        <  5:241855/149‑1 (MQ=255)
                                                                        aGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACATTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATc                                                                                        <  8:138194/132‑1 (MQ=255)
                                                                        aGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACATTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATc                                                                                        >  7:144090/1‑132 (MQ=255)
                                                                        aGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACATTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATc                                                                                        >  7:138194/1‑132 (MQ=255)
                                                                        aGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACATTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATc                                                                                        <  8:144090/132‑1 (MQ=255)
                                                                                gtCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTgcg                                                               >  3:164837/1‑149 (MQ=255)
                                                                                 tCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTgcgc                                                              <  5:316605/149‑1 (MQ=255)
                                                                                 tCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGACACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTgcg                                                               >  1:106692/1‑148 (MQ=255)
                                                                                                 tGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCAAGGCAACTGCGCACACACCTGGTTGAcc                                              >  8:184090/1‑149 (MQ=255)
                                                                                                   aaaCGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGatagc                                            >  2:291784/1‑145 (MQ=255)
                                                                                                    aaCGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATg                                                                                                     <  4:61489/91‑1 (MQ=255)
                                                                                                    aaCGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATg                                                                                                     >  3:61489/1‑91 (MQ=255)
                                                                                                     aCGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTgg                                          <  4:185791/149‑1 (MQ=255)
                                                                                                         ggCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCAc                                      >  3:65731/1‑149 (MQ=255)
                                                                                                          gCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCAcc                                     <  7:163564/149‑1 (MQ=255)
                                                                                                              gccgccGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGt                                 <  2:321192/149‑1 (MQ=255)
                                                                                                               ccgccgGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTg                                           >  8:241299/1‑138 (MQ=255)
                                                                                                               ccgccgGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTg                                           <  7:241299/138‑1 (MQ=255)
                                                                                                                  ccgGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGAt                             >  2:373854/1‑149 (MQ=255)
                                                                                                                   cgGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGACACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATg                            <  2:106692/149‑1 (MQ=255)
                                                                                                                          gaagaaGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGTCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGc                     >  7:47022/1‑149 (MQ=255)
                                                                                                                            agaagGCCTTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCt                   <  6:264648/149‑1 (MQ=255)
                                                                                                                               agTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGc                 >  5:100141/1‑148 (MQ=255)
                                                                                                                                 tCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGCAt               >  7:272604/1‑148 (MQ=255)
                                                                                                                                       cacaGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGCATACTGGt         <  4:65731/148‑1 (MQ=255)
                                                                                                                                             cTGACGTTGGCTGCCTGGAATTGATCGGCACGCGTGCCTTTGCGGGTATGGGGCAGGACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGCATACTGGTAACCgg   <  7:184090/148‑1 (MQ=255)
                                                                                                                                             cTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGCATACTGGTAACCGgc  <  7:188048/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AACAGCACGCCTCCGGCGCGATGGACAATGCACTTGGGCACGCCGGTGGTGCCACTGGAATAGAGGATGTACAGCGGGTGGTCGAACGGCAGCGGGGTGAAACGGGGCTCGCCGCCGGGCTGGAAGAAGTCATTCCACAGGCTGACGTTGGCTGCCTGGAATTCATCGGCACGCGTGCCTTTGCGGGTATGGGGCACCACGATCAGGTGCTCCAGGCCAGGCAACTGCGCACACACCTGGTTGACCTTGGTCACCTGGTCGATGGCCTTGCCTGCATACTGGTAACCGGC  >  NC_002947/3455022‑3455311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑