Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,463,544 G→C 7.9% L272V (CTG→GTG)  PP_3078 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,463,5440GC 7.9% 192.8 / 4.8 62L272V (CTG→GTG) PP_3078ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (3/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCA  >  NC_002947/3463397‑3463690
                                                                                                                                                    |                                                                                                                                                  
cGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGGGGGCAgg                                                                                                                                                   <  6:272674/149‑1 (MQ=255)
  ttGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGt                                                                                                                                                  >  5:219551/1‑148 (MQ=255)
       gCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGt                                                                                                                                             <  5:216244/148‑1 (MQ=255)
        cccAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGc                                                                                                                                           >  4:384907/1‑149 (MQ=255)
         ccAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCATGGTGGGCACGTGACCTGc                                                                                                                                           >  1:322095/1‑149 (MQ=255)
          cAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGcc                                                                                                                                          >  7:9525/1‑148 (MQ=255)
           aaGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCcca                                                                                                                                        >  6:75198/1‑149 (MQ=255)
           aaGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCcca                                                                                                                                        <  3:384907/149‑1 (MQ=255)
           aaGGTGTAGGCCGTGGCGGAGAACTCGCGGGTGTAGTCGTAGCGACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCcca                                                                                                                                        <  4:262506/149‑1 (MQ=255)
            aGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCccac                                                                                                                                       <  5:209328/149‑1 (MQ=255)
             ggTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCccacc                                                                                                                                      <  1:266294/149‑1 (MQ=255)
                gtAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATg                                                                                                                                   >  7:80738/1‑149 (MQ=255)
                 tAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCATGGTGGGCACGTGACCTGCccgccag                                                                                                                                    <  2:322095/148‑2 (MQ=255)
                 tAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGc                                                                                                                                  <  8:9525/149‑1 (MQ=255)
                  aGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCTCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGt                                                                                                                                             >  5:397063/1‑137 (MQ=255)
                  aGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGt                                                                                                                                             <  6:397063/137‑1 (MQ=255)
                   ggCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGcc                                                                                                                                 <  6:286263/148‑1 (MQ=255)
                   ggCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGcc                                                                                                                                 <  5:75198/148‑1 (MQ=255)
                   ggCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCg                                                                                                                                <  8:126987/149‑1 (MQ=255)
                       gTGGCGGTGTACTCGCGGTTGTAGTCGTATGCACCTCCTTTGAGTACCTGGCGAGCCATTTATGGTTCTCAGAAGTAATCGATGCGCTGTTTGTCTAATTTGTAGCGGCCACGTC‑TTGCGTGCAGTTCACTTGCCCAACATTCAGGGTc                                                                                                                            <  4:390562/149‑1 (MQ=255)
                       gTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTc                                                                                                                            <  7:167346/149‑1 (MQ=255)
                           cGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGt                                                                                                                        <  6:341873/149‑1 (MQ=255)
                                     gcgGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTg                                                                                                               <  5:108896/148‑1 (MQ=255)
                                               tCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCggg                                                                                                                              >  6:209215/1‑123 (MQ=255)
                                               tCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCggg                                                                                                                              <  5:209215/123‑1 (MQ=255)
                                                cGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGcc                                                                                                    <  6:219551/148‑1 (MQ=255)
                                                     ccACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTc                                                                                                                            >  8:97108/1‑119 (MQ=255)
                                                     ccACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTc                                                                                                                            <  7:97108/119‑1 (MQ=255)
                                                                      cTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGt                                                                             <  7:146857/149‑1 (MQ=255)
                                                                         gcgcggCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGGGGGCAGGTCACGTGCCCAGCATGCCGGGTCGCGTTCGTAGGTGATGCTGCGGCCGTTGTCGATGGGCCCAATGCGGTATg                                                                          <  5:195442/149‑1 (MQ=255)
                                                                         gcgcgcCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGCTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATg                                                                          <  5:189553/149‑1 (MQ=255)
                                                                          tcgagtaCTTCGGCGACACCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATgg                                                                         >  3:8928/7‑149 (MQ=255)
                                                                           gcgcCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATg                                                                                          >  8:9917/1‑131 (MQ=255)
                                                                           gcgcCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATg                                                                                          <  7:9917/131‑1 (MQ=255)
                                                                              ccACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCg                                                                     >  7:35682/1‑149 (MQ=255)
                                                                               cACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGc                                                                    >  8:374088/1‑149 (MQ=255)
                                                                                 cTTCGGCGACACCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCGCCCGGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTg                                                                  >  1:131681/1‑149 (MQ=255)
                                                                                  ttCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGc                                                                 >  4:344290/1‑149 (MQ=255)
                                                                                       tgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATgg                                                                         >  8:135398/1‑136 (MQ=255)
                                                                                       tgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATgg                                                                         <  7:135398/136‑1 (MQ=255)
                                                                                       tgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACt                                                            <  7:374088/149‑1 (MQ=255)
                                                                                         tCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTgg                                                          >  5:212251/1‑149 (MQ=255)
                                                                                            ccGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACt                                                            <  1:150436/144‑1 (MQ=255)
                                                                                            ccGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACt                                                            >  2:150436/1‑144 (MQ=255)
                                                                                              gAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGt                                                     >  7:83416/1‑149 (MQ=255)
                                                                                                    cTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGc                                               >  7:149030/1‑149 (MQ=255)
                                                                                                       gATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                            >  6:16847/1‑149 (MQ=255)
                                                                                                       gATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                            >  6:1634/1‑149 (MQ=255)
                                                                                                            gcAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCg                                       >  7:161695/1‑149 (MQ=255)
                                                                                                             cAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGt                                      >  6:183851/1‑149 (MQ=255)
                                                                                                             cAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGt                                      >  2:315491/1‑149 (MQ=255)
                                                                                                               gTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTg                                    >  2:127923/1‑149 (MQ=255)
                                                                                                                      gAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGt                             >  6:11315/1‑149 (MQ=255)
                                                                                                                      gAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGt                             >  2:261666/1‑149 (MQ=255)
                                                                                                                               gCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                            >  7:239714/1‑125 (MQ=255)
                                                                                                                               gCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                            <  8:239714/125‑1 (MQ=255)
                                                                                                                                      cGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTc             <  6:212251/149‑1 (MQ=255)
                                                                                                                                          atggtggGCACGTGACCTGCCCGCCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTg          >  4:305314/2‑149 (MQ=255)
                                                                                                                                          atggtggGCACGTGACCTGCCCGCCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTg          >  2:56868/2‑149 (MQ=255)
                                                                                                                                              cGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCg      <  5:16847/149‑1 (MQ=255)
                                                                                                                                                ggCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCGGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCggg    <  8:80738/149‑1 (MQ=255)
                                                                                                                                                 gCACGTGACCTGCCCGCCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGc   <  1:56868/149‑1 (MQ=255)
                                                                                                                                                  cAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCTGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCa  >  6:367257/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCA  >  NC_002947/3463397‑3463690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑