Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	42,123	C→T	5.9%	R23C (CGC→TGC)	PP_0039 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	42,123	0	C	T	5.9%	201.3 / 2.3	67	R23C (CGC→TGC)	PP_0039	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/29);  new base T (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TTGAACCTGATTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTACGCGGCGGTCGAG  >  NC_002947/41975‑42269                                                                                                                                                     |                                                                                                                                                   ttGAACCTGATTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATATTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGgcc                                                                                                                                                    >  6:288822/1‑149 (MQ=255)    aaCCTGATTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGgccgc                                                                                                                                                  <  6:258379/148‑1 (MQ=255)     aCCTGATTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGccc                                                                                                                                                >  4:263222/1‑149 (MQ=255)      ccTGATTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCg                                                                                                                                               >  8:338942/1‑149 (MQ=255)          aTTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATATTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTa                                                                                                                                             <  5:288822/147‑1 (MQ=255)               aCGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCACCCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCAt                                                                                                                                      >  7:79078/1‑149 (MQ=255)                 gTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCCTCTGGCCGCCCGTACTCTCATCt                                                                                                                                    >  2:12943/1‑149 (MQ=255)                 gTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCCTCTGGCCGCCCGTACTCTCATCt                                                                                                                                    >  2:19347/1‑149 (MQ=255)                     aGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCATCTGGCCGCCCGTACTCTCATCTGCAc                                                                                                                                >  3:306346/1‑149 (MQ=255)                       cTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGc                                                                                                                                  >  1:205518/1‑145 (MQ=255)                       cTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGc                                                                                                                                  <  2:205518/145‑1 (MQ=255)                           gcgTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTccac                                                                                                                          <  3:378535/149‑1 (MQ=255)                              tCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAg                                                                                                                       <  3:250882/149‑1 (MQ=255)                               cTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCTGCCCGTACTCTCATCTGCAc                                                                                                                                <  5:177283/139‑1 (MQ=255)                               cTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCTGCCCGTACTCTCATCTGCAc                                                                                                                                >  6:177283/1‑139 (MQ=255)                                tAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCt                                                                                                                     >  6:367093/1‑149 (MQ=255)                                 aaCGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTg                                                                                                                    >  8:82515/1‑149 (MQ=255)                                 aaCGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTg                                                                                                                    >  3:33932/1‑149 (MQ=255)                                    ggCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTc                                                                                                                 <  3:263222/149‑1 (MQ=255)                                    ggCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTc                                                                                                                 <  4:49779/149‑1 (MQ=255)                                             aGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGc                                                                                                         >  8:114022/1‑148 (MQ=255)                                               cgcAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCAt                                                                                                      <  7:338942/149‑1 (MQ=255)                                                       ccccAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCg                                                                                              >  6:220831/1‑149 (MQ=255)                                                        cccAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGt                                                                                             <  3:282864/149‑1 (MQ=255)                                                             cGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCAc                                                                                        <  5:367093/149‑1 (MQ=255)                                                             cGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTCCAc                                                                                        <  7:82515/149‑1 (MQ=255)                                                                     aTTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGtt                                                                                <  5:345629/149‑1 (MQ=255)                                                                      ttAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTg                                                                               <  7:114022/149‑1 (MQ=255)                                                                      ttAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTg                                                                               >  8:323085/1‑149 (MQ=255)                                                                      ttAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCCTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGTCCATCGGCTCCGTTGCACCGCAAGTTg                                                                               <  1:19347/149‑1 (MQ=255)                                                                      ttAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCCTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTg                                                                               <  1:12943/149‑1 (MQ=255)                                                                          gggATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCAc                                                                                        <  5:150916/136‑1 (MQ=255)                                                                          gggATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCAc                                                                                        >  6:150916/1‑136 (MQ=255)                                                                          gggATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCAt                                                                           >  4:368346/1‑149 (MQ=255)                                                                          gggATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCAt                                                                           >  6:31953/1‑149 (MQ=255)                                                                            gATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATcc                                                                         <  7:323085/149‑1 (MQ=255)                                                                            gATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATc                                                                          >  8:364401/1‑148 (MQ=255)                                                                              tAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCaa                                                                       <  5:220831/149‑1 (MQ=255)                                                                               aGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCCCCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCCCCGCAAGTTGCCATcc                                                                         >  6:417786/1‑146 (MQ=255)                                                                                   aTGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACaa                                                                  >  6:270086/1‑149 (MQ=255)                                                                                      ccTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCaa                                                                                   >  8:70562/1‑129 (MQ=255)                                                                                      ccTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCaa                                                                                   <  7:70562/129‑1 (MQ=255)                                                                                        tGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGc                                                             >  6:157266/1‑149 (MQ=255)                                                                                         gCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGc                                                             >  5:380688/1‑148 (MQ=255)                                                                                          cAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCTGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGcc                                                            >  6:64891/1‑148 (MQ=255)                                                                                          cAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGACGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTACACGCCGCCATCGGCTACGTAGAACCGCAAGTTGCCATCCAAGAAAACCTGcc                                                            >  4:206698/1‑148 (MQ=255)                                                                                             aaaaGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCTGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAAtt                                                        <  5:64891/149‑1 (MQ=255)                                                                                               aaGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTTCCAAtt                                                        >  6:338455/1‑147 (MQ=255)                                                                                                aGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCAGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAg                                                     <  2:53092/149‑1 (MQ=255)                                                                                                 gctgctTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAg                                                                                  <  3:239745/119‑1 (MQ=255)                                                                                                 gctgctTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAg                                                                                  >  4:239745/1‑119 (MQ=255)                                                                                                   tgctTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTc                                                  >  2:72518/1‑149 (MQ=255)                                                                                                     ctTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCt                                                 <  5:157266/148‑1 (MQ=255)                                                                                                         aaCCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCg                                             <  6:32150/148‑1 (MQ=255)                                                                                                           ccTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGa                                          <  5:31953/149‑1 (MQ=255)                                                                                                           ccTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGa                                          >  3:387244/1‑149 (MQ=255)                                                                                                           ccTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGa                                          <  7:99925/149‑1 (MQ=255)                                                                                                                   tgctCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACgaga                                  >  4:357311/1‑149 (MQ=255)                                                                                                                      tCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGacac                               >  6:124699/1‑149 (MQ=255)                                                                                                                      tCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATAAAGTTCTGTCGTGACAACGAGacac                               >  3:118357/1‑149 (MQ=255)                                                                                                                            gCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAg                          >  5:322663/1‑148 (MQ=255)                                                                                                                                gCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACaa                     >  2:119270/1‑149 (MQ=255)                                                                                                                                 ccTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAAc                    <  3:368346/149‑1 (MQ=255)                                                                                                                                  cTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGc                                                             >  4:207623/1‑107 (MQ=255)                                                                                                                                  cTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGc                                                             <  3:207623/107‑1 (MQ=255)                                                                                                                                      ttCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCCCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTa               <  3:316103/149‑1 (MQ=255)                                                                                                                                           ggCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTACgcgg          <  4:273684/149‑1 (MQ=255)                                                                                                                                           ggCTTCTGGCCGCCAGTACTCTCATCTGCACCTCAACCCGCTGTTCCCCGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACTAGACACGGCAGTACAACCTCTACgcgg          <  8:79078/149‑1 (MQ=255)                                                                                                                                                cTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTACGCGGCGGTc     >  4:358070/1‑149 (MQ=255)                                                                                                                                                   gccgccCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTACGCGGCGGTCg    <  4:33932/147‑1 (MQ=255)                                                                                                                                                   gccgccCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTACGCGGCGGTCGAg  >  4:380380/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTGAACCTGATTCGACGTCCAGCTTCGCGTCTAACGGCCCTACAAGCGCAGCTACCCCAACGTCGCTCATTAGGGGATAGTCATGCCTGCAAAAAAGCTGCTTCAACCTCTCGCTGCTCAGCTGCACGCCTCGTTCTCGGCTTCTGGCCGCCCGTACTCTCATCTGCACCTCCACCAGCTGTTCCACGCGGCCATCGGCTCCGTTGCACCGCAAGTTGCCATCCAAGACAAGCTGCCAATTCAGGTCTGTCGTGACAACGAGACACGGCAGTACAACCTCTACGCGGCGGTCGAG  >  NC_002947/41975‑42269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑