| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,534,777 | C→A | 8.0% | intergenic (+64/‑91) | atoB → / → PP_3124 | 3‑oxoacid CoA‑transferase subunit B/short chain fatty acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,534,777 | 0 | C | A | 8.0% | 178.7 / 4.1 | 58 | intergenic (+64/‑91) | atoB/PP_3124 | 3‑oxoacid CoA‑transferase subunit B/short chain fatty acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (25/28); new base A (3/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01 | |||||||||||
GGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTGCGCTGCT > NC_002947/3534634‑3534919 | gggTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACcgccgc < 7:314128/147‑1 (MQ=255) aGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTcgcg < 8:138071/149‑1 (MQ=255) gCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCgg < 7:196560/149‑1 (MQ=255) cGTCGAGGAAATCATCGAGAAAACCGCCCGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCAACCTGTAGGCCCGGCTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCCCggg > 4:189575/1‑149 (MQ=255) gAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTaa < 8:46826/148‑1 (MQ=255) aGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGc < 2:312026/149‑1 (MQ=255) aaTCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGt < 1:221327/149‑1 (MQ=255) tcatcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTc > 5:31604/1‑149 (MQ=255) catcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTc > 1:145377/1‑148 (MQ=255) atcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCt < 3:103842/149‑1 (MQ=255) tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTa > 1:66336/1‑149 (MQ=255) tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTa < 8:147704/149‑1 (MQ=255) gagaAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACa < 2:66336/149‑1 (MQ=255) agaAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAg > 1:156025/1‑149 (MQ=255) aaCCGCCGGCAAACTGATCGTGCCGGATGATGTAGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGtt > 1:254810/1‑149 (MQ=255) ccgGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGc < 6:31604/149‑1 (MQ=255) ccgGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGc < 2:145377/149‑1 (MQ=255) ccgGCAAACTGATCGGGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGc < 8:350360/149‑1 (MQ=255) cTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTg < 4:307540/149‑1 (MQ=255) tGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTgg > 4:87679/1‑149 (MQ=255) gatgT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGGTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCa > 7:152728/1‑148 (MQ=255) tgt‑gAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATc < 7:2200/148‑1 (MQ=255) t‑gAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCa > 5:387456/1‑149 (MQ=255) gAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTg > 3:330743/1‑129 (MQ=255) gAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTg < 4:330743/129‑1 (MQ=255) aaGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCAt > 8:352196/1‑148 (MQ=255) aaGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCAt > 6:85745/1‑148 (MQ=255) aGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATaa < 8:256393/149‑1 (MQ=255) ggAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGtt > 5:170819/1‑102 (MQ=255) ggAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGtt < 6:170819/102‑1 (MQ=255) ggAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATc < 5:173949/142‑1 (MQ=255) ggAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATc > 6:173949/1‑142 (MQ=255) aaaTGACCTTCTAAGGCATGACGTGATCTCACCCTTTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGAAATACCGCTTCATCCCATAAAAc < 1:44052/149‑1 (MQ=255) aaaTGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAAc < 2:254810/149‑1 (MQ=255) ttCTAATGCATGACGTGATCTAACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGTTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATaaaa < 3:189575/149‑1 (MQ=255) tAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTTACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGAGTTGACTGGCAATACCGCTTCATCACATAAAACCAATaaaatg > 1:131860/1‑146 (MQ=255) aGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGaa < 7:352196/148‑1 (MQ=255) aCGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCg > 3:345653/1‑149 (MQ=255) cGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGt < 3:87679/149‑1 (MQ=255) cACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCt > 2:51483/1‑148 (MQ=255) cTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAAt < 6:387456/149‑1 (MQ=255) gcgGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAAcc > 2:327350/1‑127 (MQ=255) gcgGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAAcc < 1:327350/127‑1 (MQ=255) gggTTTACCCGCGAATGCGTCTGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGc < 8:152728/149‑1 (MQ=255) ccGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAACCCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgc > 8:218143/1‑149 (MQ=255) ccGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgc > 5:200516/1‑149 (MQ=255) ccGCGAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAACCCGCGCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgc > 6:330488/1‑149 (MQ=25) cgcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGc > 4:320364/1‑121 (MQ=255) cgcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGc < 3:320364/121‑1 (MQ=255) cgcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcg > 3:98837/1‑149 (MQ=255) gcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcgc > 8:327070/1‑149 (MQ=255) gcgAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcgc > 2:284982/1‑149 (MQ=255) gcgAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAACCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCAGCTCcgcgc > 8:318848/1‑149 (MQ=37) gcgAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAACCCGCGCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAAt > 7:27423/1‑100 (MQ=17) gcgAATGCGGCGGTCAATGTACCGACGCATTCGCGGGTAAACCCGCGCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAAt < 8:27423/100‑1 (MQ=17) tGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCttt < 7:327070/149‑1 (MQ=255) tCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCACCCGTGGCCGCTGAAATTCAAGACAGCCGCTACGCGCGCTTTGCCt > 7:237767/1‑149 (MQ=255) gTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTgc < 6:200516/149‑1 (MQ=255) taCCGACGCATTCGCGGGTAAACCCGCGCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTGCgctgct < 5:330488/147‑1 (MQ=39) | GGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGT‑GAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTGACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTTGCCTTGCGCTGCT > NC_002947/3534634‑3534919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |