Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,536,544 C→G 7.1% A61G (GCC→GGC)  PP_3125 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,536,5440CG 7.1% 247.3 / 4.8 79A61G (GCC→GGC) PP_3125Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (38/35);  new base G (2/4);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAGCC  >  NC_002947/3536401‑3536688
                                                                                                                                                |                                                                                                                                                 
gATGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCg                                                                                                                                                 >  8:103726/1‑146 (MQ=255)
 aTGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAaga                                                                                                                                             <  3:268503/149‑1 (MQ=255)
    aCCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGc                                                                                                                                           <  6:9692/148‑1 (MQ=255)
     cccTGGAAATGCTGGCCGAAAAGACTGGCATGCCCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGctc                                                                                                                                         <  7:325371/149‑3 (MQ=255)
      ccTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAgg                                                                                                                                        >  7:265869/1‑149 (MQ=255)
      ccTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAgg                                                                                                                                        >  7:270367/1‑149 (MQ=255)
      ccTGGAAATGCTGGCCGAAAAGACTGGCAGGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCGCTCGCTCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCGGCGCGGCGGGGTCGAAGAGCTCTGCGGCGAAGAGCAgg                                                                                                                                        >  6:414395/1‑149 (MQ=255)
        tGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTACAAGGTCGAGCGCGGCCTCAACACGCCCTCGGTCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGcc                                                                                                                                      >  6:110939/1‑149 (MQ=255)
          gAAATGCTGGCCGAAAAGACTGGCATGACCAAAGGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCgg                                                                                                                                    <  5:196609/149‑1 (MQ=255)
          gAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGctcttc                                                                                                                                      <  8:264691/147‑6 (MQ=255)
           aaaTGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACCTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCgg                                                                                                                                    <  1:110646/149‑1 (MQ=255)
                   gCCGAAAAGACTGGCATGACCATAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCATCACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGc                                                                                                                             <  5:133218/147‑1 (MQ=255)
                   gCCGAAAAGACTGGCATGACCAAAAGCTACCTGTACAAGGTCGAGCGCGGCCTCAACACGCCCTCGGTCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGc                                                                                                                             <  5:110939/147‑1 (MQ=255)
                            aCTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCt                                                                                                                  >  2:67164/1‑149 (MQ=255)
                             cTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTg                                                                                                                 <  8:265869/149‑1 (MQ=255)
                              tGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTgg                                                                                                                >  6:179105/1‑149 (MQ=255)
                                gCATGACCAAAAGCTACCTGTCCAAGGTCGAGAGAGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGATCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTg                                                                                                              >  5:309853/1‑149 (MQ=255)
                                  aTGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTgcg                                                                                                            >  4:109344/1‑149 (MQ=255)
                                       cAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTa                                                                                                       >  3:125391/1‑149 (MQ=255)
                                       cAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTa                                                                                                       <  8:334532/149‑1 (MQ=255)
                                        aaaaGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGCGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTAc                                                                                                      >  5:312935/1‑149 (MQ=255)
                                         aaaGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGctc                                                                                                                                         >  5:4683/1‑111 (MQ=255)
                                         aaaGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGctc                                                                                                                                         <  6:4683/113‑3 (MQ=255)
                                            gCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAg                                                                                                  >  8:266804/1‑149 (MQ=255)
                                             cTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAgc                                                                                                 <  8:274811/149‑1 (MQ=255)
                                                   gTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGATGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAgg                                                                                           >  5:335752/1‑149 (MQ=255)
                                                      cAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGcc                                                                                         <  7:103726/148‑1 (MQ=255)
                                                         ggTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑gg                                                                                    <  4:129436/149‑1 (MQ=255)
                                                             gAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTgg                                                                                <  4:125391/149‑1 (MQ=255)
                                                             gAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTgg                                                                                >  2:390081/1‑149 (MQ=255)
                                                                 gcgGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGAt                                                                            <  7:65352/149‑1 (MQ=255)
                                                                       tCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGGGAGGCGATGGGc                                                                        >  1:343664/1‑148 (MQ=255)
                                                                        cAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAg                                                                     >  7:150273/1‑149 (MQ=255)
                                                                         aaCACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAACTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATgg                                                                          <  4:168184/143‑1 (MQ=255)
                                                                         aaCACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAACTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATgg                                                                          >  3:168184/1‑143 (MQ=255)
                                                                           cacGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGc                                                                  >  4:119107/1‑149 (MQ=255)
                                                                             cGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCg                                                                <  5:179105/149‑1 (MQ=255)
                                                                               cccTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCggg                                                              <  3:145130/149‑1 (MQ=255)
                                                                               cccTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCggg                                                              >  4:145130/1‑149 (MQ=255)
                                                                                 cTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCgggg                                                             >  7:359083/1‑148 (MQ=255)
                                                                                  tCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTa                                                           >  2:379441/1‑149 (MQ=255)
                                                                                  tCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGACGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTa                                                           >  1:294730/1‑149 (MQ=255)
                                                                                    gATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTAc                                                          <  3:119107/148‑1 (MQ=255)
                                                                                        gCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCgg                                                      <  8:359083/148‑1 (MQ=255)
                                                                                        gCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGc                                                     >  6:226303/1‑149 (MQ=255)
                                                                                         cTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGcc                                                    <  1:379441/149‑1 (MQ=255)
                                                                                            cggcgCTGATA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCTAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCC‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCt                                                  <  1:67164/148‑1 (MQ=255)
                                                                                            cggcgCTGAAA‑CTGGCGCGGGCGCTGAACGTCAATCGCGACGAGCTCATCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTc                                                 <  5:414395/149‑1 (MQ=255)
                                                                                              gcgcTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCAc                                               <  7:266804/149‑1 (MQ=255)
                                                                                               cgcTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCggg                                                                                                                                   <  7:191768/65‑1 (MQ=255)
                                                                                               cgcTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCggg                                                                                                                                   >  8:191768/1‑65 (MQ=255)
                                                                                                gcTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCAc                                               >  6:100921/1‑147 (MQ=255)
                                                                                                   gAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCAc                                               <  1:256722/144‑1 (MQ=255)
                                                                                                   gAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCAc                                               >  2:256722/1‑144 (MQ=255)
                                                                                                    aaa‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGcc                                                    <  5:124384/138‑1 (MQ=255)
                                                                                                    aaa‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGcc                                                    >  6:124384/1‑138 (MQ=255)
                                                                                                    aaa‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTc                                          <  5:226303/148‑1 (MQ=255)
                                                                                                         tGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAgg                                                                    >  6:330965/1‑118 (MQ=255)
                                                                                                         tGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAgg                                                                    <  5:330965/118‑1 (MQ=255)
                                                                                                         tGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGAtt                                     >  1:400925/1‑149 (MQ=255)
                                                                                                            ggAGGGCGCTGTACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGc                                  <  6:309853/148‑1 (MQ=255)
                                                                                                            cGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGc                                  <  6:312935/149‑1 (MQ=255)
                                                                                                             gAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGa                                       >  7:258787/1‑143 (MQ=255)
                                                                                                             gAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGa                                       <  8:258787/143‑1 (MQ=255)
                                                                                                                    cTGAACGGCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAg                          <  8:270367/149‑1 (MQ=255)
                                                                                                                     tGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGc                         >  5:372832/1‑149 (MQ=255)
                                                                                                                     tGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGc                         >  3:381996/1‑149 (MQ=255)
                                                                                                                     tGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGc                         >  3:46413/1‑149 (MQ=255)
                                                                                                                          gTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCctgct                    >  6:291038/1‑149 (MQ=255)
                                                                                                                                   gAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGTCGTTTc            <  5:291038/148‑1 (MQ=255)
                                                                                                                                   gAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTc            <  5:100921/148‑1 (MQ=255)
                                                                                                                                   gAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCt           >  5:332370/1‑149 (MQ=255)
                                                                                                                                       agCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGAtt       <  2:294730/149‑1 (MQ=255)
                                                                                                                                        gCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGAtt       >  8:209041/1‑148 (MQ=255)
                                                                                                                                            ttCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCAc                                               >  3:352685/1‑104 (MQ=255)
                                                                                                                                            ttCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCAc                                               <  4:352685/104‑1 (MQ=255)
                                                                                                                                            ttCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgcc  >  4:24827/1‑149 (MQ=255)
                                                                                                                                            ttCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgcc  >  3:229476/1‑149 (MQ=255)
                                                                                                                                              cGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgcc  <  6:372832/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAA‑CTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCT‑GGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAGCC  >  NC_002947/3536401‑3536688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑