Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,554,063 A→T 9.2% I244F (ATC→TTC)  PP_3138 → VirK domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,554,0630AT 9.2% 184.4 / 8.2 65I244F (ATC→TTC) PP_3138VirK domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (33/26);  new base T (2/4);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

ACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAT  >  NC_002947/3553915‑3554203
                                                                                                                                                    |                                                                                                                                            
aCGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGa                                                                                                                                              <  8:59831/149‑1 (MQ=255)
aCGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGa                                                                                                                                              >  6:402423/1‑149 (MQ=255)
      ccTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTgg                                                                                                                                         >  6:296189/1‑148 (MQ=255)
      ccTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGc                                                                                                                                        <  5:5433/149‑1 (MQ=255)
      ccTGTTGCTCGAAGCGTTGAAATGCCTGGGAAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGc                                                                                                                                        <  2:77137/149‑1 (MQ=255)
              tCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGAtcac                                                                                                                                >  3:236197/1‑149 (MQ=255)
              tCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAacac                                                                                                                                >  2:90991/1‑149 (MQ=255)
                gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCATCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgt                                                                                                                              <  6:338307/149‑2 (MQ=255)
                gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACgg                                                                                                                              >  4:147598/1‑149 (MQ=255)
                gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACg                                                                                                                               <  1:90991/148‑1 (MQ=255)
                 aaGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             <  4:236197/149‑3 (MQ=255)
                 aaGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGt                                                                                                                             >  6:391553/1‑149 (MQ=255)
                  atcGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCGTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             <  8:334420/146‑3 (MQ=255)
                      ttGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             <  3:278308/144‑3 (MQ=255)
                      ttGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             >  4:278308/1‑142 (MQ=255)
                        gAAATGCCTGGGCAGAACCTTGGGTGTTACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATAGCCATCCCTATTTTTGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAg                                                                                                                      >  1:12451/1‑149 (MQ=255)
                        gAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGCTgtggcac                                                                                                                      >  5:60198/1‑143 (MQ=255)
                          aaTGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGc                                                                                                                     <  3:337988/148‑1 (MQ=255)
                                   gCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt                                                                                                           >  6:18446/1‑149 (MQ=255)
                                    cAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGCCAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt                                                                                                           >  4:318454/1‑148 (MQ=255)
                                     aGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt                                                                                                           >  8:283103/1‑147 (MQ=255)
                                     aGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGa                                                                                                         <  7:283103/149‑1 (MQ=255)
                                       aaCCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAg                                                                                                        >  6:173271/1‑148 (MQ=255)
                                               gtgtGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTc                                                                                               <  5:18446/149‑1 (MQ=255)
                                                 gtgACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTctg                                                                                             >  5:83220/1‑147 (MQ=255)
                                                    acacAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCt                                                                                          >  2:66729/1‑149 (MQ=255)
                                                     cacaGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTg                                                                                         <  1:66729/149‑1 (MQ=255)
                                                        aGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTg                                                                                                                                          >  3:212633/1‑97 (MQ=255)
                                                        aGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTg                                                                                                                                          <  4:212633/97‑1 (MQ=255)
                                                             tATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTa                                                                                   <  5:296189/147‑1 (MQ=255)
                                                              gtGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAgcg                                                                                >  6:96549/2‑149 (MQ=255)
                                                                gCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGcc                                                                              >  2:180629/1‑149 (MQ=255)
                                                                   gTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGgc                                                                           >  4:231407/1‑149 (MQ=255)
                                                                           cGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACggg                                                                   <  5:396097/149‑1 (MQ=255)
                                                                                gCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGa                                                               >  5:360732/1‑148 (MQ=255)
                                                                                 ccGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGaaa                                                             <  5:96549/149‑1 (MQ=255)
                                                                                   ggCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACt                                                           <  6:298064/149‑1 (MQ=255)
                                                                                     cACCATCGCCATCCCTATTTTGGCAGCTACAAGGGCCAGGACCTGGCTGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACt                                                           <  5:402423/147‑1 (MQ=255)
                                                                                         aTCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCgg                                                                >  5:378957/1‑138 (MQ=255)
                                                                                         aTCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCgg                                                                <  6:378957/138‑1 (MQ=255)
                                                                                             ccATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg                                                 >  3:372457/1‑149 (MQ=255)
                                                                                                  ccTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc                                                <  6:377535/145‑1 (MQ=255)
                                                                                                  ccTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc                                                >  5:377535/1‑145 (MQ=255)
                                                                                                   cTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAg                                            >  6:23587/1‑148 (MQ=255)
                                                                                                        ttGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      >  4:140317/1‑149 (MQ=255)
                                                                                                         tGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      <  3:147598/148‑1 (MQ=255)
                                                                                                           gCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGc                                    <  6:360732/148‑1 (MQ=255)
                                                                                                            cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCg                                   <  2:98058/148‑1 (MQ=255)
                                                                                                            cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCg                                   >  1:98058/1‑148 (MQ=255)
                                                                                                            cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGAAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGa                                  >  8:175714/1‑149 (MQ=255)
                                                                                                            cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGA‑‑TGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATg                                >  5:15445/1‑149 (MQ=255)
                                                                                                             aGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGAt                                 >  5:91469/1‑149 (MQ=255)
                                                                                                             aGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGAt                                 <  2:12451/149‑1 (MQ=255)
                                                                                                                  cAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTAcc                            <  5:391553/149‑1 (MQ=255)
                                                                                                                         cAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACATCGTCTGACCGTGAGGATTTTTTAATCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATACCGGCCAAGAAACGGGCGATGTTccgccgccg                     >  1:305167/1‑149 (MQ=255)
                                                                                                                               cTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAgg                >  6:111022/1‑148 (MQ=255)
                                                                                                                                    ggcCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg                                                 <  6:226740/110‑1 (MQ=255)
                                                                                                                                    ggcCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg                                                 >  5:226740/1‑110 (MQ=255)
                                                                                                                                      ccAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTc        <  4:372457/149‑1 (MQ=255)
                                                                                                                                       cAACTACGACGTGATCGGGCAAGAACACGGTGCCACAGCGGCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcg       <  8:229813/149‑1 (MQ=255)
                                                                                                                                         aCTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      >  3:348125/1‑116 (MQ=255)
                                                                                                                                         aCTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      <  4:348125/116‑1 (MQ=255)
                                                                                                                                          cTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcgac     >  1:384862/1‑148 (MQ=255)
                                                                                                                                             cgacgTGATCTCGCAAGAACACGGTGCCACTGCGGCTGACCGTGAGTATGTTTGCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGAGTTCCCGTCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAt  <  5:267794/148‑1 (MQ=255)
                                                                                                                                               acgTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc                                                >  8:38285/1‑100 (MQ=255)
                                                                                                                                                   gATCTGGCAAGAACACGGTGCCACAGCGTCTGA‑CATGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcga      <  6:156969/137‑1 (MQ=255)
                                                                                                                                                   gATCTGGCAAGAACACGGTGCCACAGCGTCTGA‑CATGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcga      >  5:156969/1‑137 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
ACGCAGCCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAT  >  NC_002947/3553915‑3554203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑