Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,554,067 G→T 9.8% W245L (TGG→TTG)  PP_3138 → VirK domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,554,0670GT 9.8% 178.3 / 9.2 62W245L (TGG→TTG) PP_3138VirK domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (31/24);  new base T (2/4);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAT  >  NC_002947/3553921‑3554203
                                                                                                                                                  |                                                                                                                                        
ccTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTgg                                                                                                                                         >  6:296189/1‑148 (MQ=255)
ccTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGc                                                                                                                                        <  5:5433/149‑1 (MQ=255)
ccTGTTGCTCGAAGCGTTGAAATGCCTGGGAAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGc                                                                                                                                        <  2:77137/149‑1 (MQ=255)
        tCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGAtcac                                                                                                                                >  3:236197/1‑149 (MQ=255)
        tCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAacac                                                                                                                                >  2:90991/1‑149 (MQ=255)
          gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCATCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgt                                                                                                                              <  6:338307/149‑2 (MQ=255)
          gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACgg                                                                                                                              >  4:147598/1‑149 (MQ=255)
          gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACg                                                                                                                               <  1:90991/148‑1 (MQ=255)
           aaGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             <  4:236197/149‑3 (MQ=255)
           aaGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGt                                                                                                                             >  6:391553/1‑149 (MQ=255)
            atcGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCGTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             <  8:334420/146‑3 (MQ=255)
                ttGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             <  3:278308/144‑3 (MQ=255)
                ttGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc                                                                                                                             >  4:278308/1‑142 (MQ=255)
                  gAAATGCCTGGGCAGAACCTTGGGTGTTACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATAGCCATCCCTATTTTTGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAg                                                                                                                      >  1:12451/1‑149 (MQ=255)
                  gAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGCTgtggcac                                                                                                                      >  5:60198/1‑143 (MQ=255)
                    aaTGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGc                                                                                                                     <  3:337988/148‑1 (MQ=255)
                             gCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt                                                                                                           >  6:18446/1‑149 (MQ=255)
                              cAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGCCAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt                                                                                                           >  4:318454/1‑148 (MQ=255)
                               aGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt                                                                                                           >  8:283103/1‑147 (MQ=255)
                               aGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGa                                                                                                         <  7:283103/149‑1 (MQ=255)
                                 aaCCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAg                                                                                                        >  6:173271/1‑148 (MQ=255)
                                         gtgtGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTc                                                                                               <  5:18446/149‑1 (MQ=255)
                                           gtgACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTctg                                                                                             >  5:83220/1‑147 (MQ=255)
                                              acacAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCt                                                                                          >  2:66729/1‑149 (MQ=255)
                                               cacaGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTg                                                                                         <  1:66729/149‑1 (MQ=255)
                                                  aGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTg                                                                                                                                          <  4:212633/97‑1 (MQ=255)
                                                  aGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTg                                                                                                                                          >  3:212633/1‑97 (MQ=255)
                                                       tATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTa                                                                                   <  5:296189/147‑1 (MQ=255)
                                                        gtGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAgcg                                                                                >  6:96549/2‑149 (MQ=255)
                                                          gCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGcc                                                                              >  2:180629/1‑149 (MQ=255)
                                                             gTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGgc                                                                           >  4:231407/1‑149 (MQ=255)
                                                                     cGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACggg                                                                   <  5:396097/149‑1 (MQ=255)
                                                                          gCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGa                                                               >  5:360732/1‑148 (MQ=255)
                                                                           ccGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGaaa                                                             <  5:96549/149‑1 (MQ=255)
                                                                             ggCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACt                                                           <  6:298064/149‑1 (MQ=255)
                                                                               cACCATCGCCATCCCTATTTTGGCAGCTACAAGGGCCAGGACCTGGCTGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACt                                                           <  5:402423/147‑1 (MQ=255)
                                                                                   aTCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCgg                                                                <  6:378957/138‑1 (MQ=255)
                                                                                   aTCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCgg                                                                >  5:378957/1‑138 (MQ=255)
                                                                                       ccATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg                                                 >  3:372457/1‑149 (MQ=255)
                                                                                            ccTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc                                                >  5:377535/1‑145 (MQ=255)
                                                                                            ccTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc                                                <  6:377535/145‑1 (MQ=255)
                                                                                             cTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAg                                            >  6:23587/1‑148 (MQ=255)
                                                                                                  ttGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      >  4:140317/1‑149 (MQ=255)
                                                                                                   tGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      <  3:147598/148‑1 (MQ=255)
                                                                                                     gCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGc                                    <  6:360732/148‑1 (MQ=255)
                                                                                                      cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCg                                   <  2:98058/148‑1 (MQ=255)
                                                                                                      cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCg                                   >  1:98058/1‑148 (MQ=255)
                                                                                                      cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGAAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGa                                  >  8:175714/1‑149 (MQ=255)
                                                                                                      cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGA‑‑TGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATg                                >  5:15445/1‑149 (MQ=255)
                                                                                                       aGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGAt                                 >  5:91469/1‑149 (MQ=255)
                                                                                                       aGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGAt                                 <  2:12451/149‑1 (MQ=255)
                                                                                                            cAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTAcc                            <  5:391553/149‑1 (MQ=255)
                                                                                                                   cAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACATCGTCTGACCGTGAGGATTTTTTAATCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATACCGGCCAAGAAACGGGCGATGTTccgccgccg                     >  1:305167/1‑149 (MQ=255)
                                                                                                                         cTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAgg                >  6:111022/1‑148 (MQ=255)
                                                                                                                              ggcCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg                                                 >  5:226740/1‑110 (MQ=255)
                                                                                                                              ggcCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg                                                 <  6:226740/110‑1 (MQ=255)
                                                                                                                                ccAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTc        <  4:372457/149‑1 (MQ=255)
                                                                                                                                 cAACTACGACGTGATCGGGCAAGAACACGGTGCCACAGCGGCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcg       <  8:229813/149‑1 (MQ=255)
                                                                                                                                   aCTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      <  4:348125/116‑1 (MQ=255)
                                                                                                                                   aCTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg                                      >  3:348125/1‑116 (MQ=255)
                                                                                                                                    cTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcgac     >  1:384862/1‑148 (MQ=255)
                                                                                                                                       cgacgTGATCTCGCAAGAACACGGTGCCACTGCGGCTGACCGTGAGTATGTTTGCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGAGTTCCCGTCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAt  <  5:267794/148‑1 (MQ=255)
                                                                                                                                         acgTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc                                                >  8:38285/1‑100 (MQ=255)
                                                                                                                                             gATCTGGCAAGAACACGGTGCCACAGCGTCTGA‑CATGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcga      <  6:156969/137‑1 (MQ=255)
                                                                                                                                             gATCTGGCAAGAACACGGTGCCACAGCGTCTGA‑CATGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcga      >  5:156969/1‑137 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
CCTGTTGCTCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAT  >  NC_002947/3553921‑3554203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑