| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,554,070 | A→C | 9.6% | Q246P (CAA→CCA) | PP_3138 → | VirK domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,554,070 | 0 | A | C | 9.6% | 174.1 / 5.3 | 60 | Q246P (CAA→CCA) | PP_3138 | VirK domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (31/23); new base C (2/4); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAT > NC_002947/3553929‑3554203 | tCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGAtcac > 3:236197/1‑149 (MQ=255)tCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAacac > 2:90991/1‑149 (MQ=255) gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCATCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgt < 6:338307/149‑2 (MQ=255) gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACgg > 4:147598/1‑149 (MQ=255) gAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACg < 1:90991/148‑1 (MQ=255) aaGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc < 4:236197/149‑3 (MQ=255) aaGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGt > 6:391553/1‑149 (MQ=255) atcGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCGTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc < 8:334420/146‑3 (MQ=255) ttGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc < 3:278308/144‑3 (MQ=255) ttGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGTTCTTGCCAGATCACgtc > 4:278308/1‑142 (MQ=255) gAAATGCCTGGGCAGAACCTTGGGTGTTACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATAGCCATCCCTATTTTTGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAg > 1:12451/1‑149 (MQ=255) gAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGCTgtggcac > 5:60198/1‑143 (MQ=255) aaTGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGc < 3:337988/148‑1 (MQ=255) gCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt > 6:18446/1‑149 (MQ=255) cAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGCCAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt > 4:318454/1‑148 (MQ=255) aGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGt > 8:283103/1‑147 (MQ=255) aGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGa < 7:283103/149‑1 (MQ=255) aaCCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAg > 6:173271/1‑148 (MQ=255) gtgtGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTc < 5:18446/149‑1 (MQ=255) gtgACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTctg > 5:83220/1‑147 (MQ=255) acacAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCt > 2:66729/1‑149 (MQ=255) cacaGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTg < 1:66729/149‑1 (MQ=255) tATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTa < 5:296189/147‑1 (MQ=255) gtGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAgcg > 6:96549/2‑149 (MQ=255) gCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGcc > 2:180629/1‑149 (MQ=255) gTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGgc > 4:231407/1‑149 (MQ=255) cGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACggg < 5:396097/149‑1 (MQ=255) gCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGa > 5:360732/1‑148 (MQ=255) ccGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGaaa < 5:96549/149‑1 (MQ=255) ggCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACt < 6:298064/149‑1 (MQ=255) cACCATCGCCATCCCTATTTTGGCAGCTACAAGGGCCAGGACCTGGCTGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACt < 5:402423/147‑1 (MQ=255) aTCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCgg < 6:378957/138‑1 (MQ=255) aTCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCgg > 5:378957/1‑138 (MQ=255) ccATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg > 3:372457/1‑149 (MQ=255) ccTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc > 5:377535/1‑145 (MQ=255) ccTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc < 6:377535/145‑1 (MQ=255) cTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAg > 6:23587/1‑148 (MQ=255) ttGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg > 4:140317/1‑149 (MQ=255) tGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg < 3:147598/148‑1 (MQ=255) gCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGc < 6:360732/148‑1 (MQ=255) cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCg < 2:98058/148‑1 (MQ=255) cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCg > 1:98058/1‑148 (MQ=255) cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGAAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGa > 8:175714/1‑149 (MQ=255) cAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGA‑‑TGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATg > 5:15445/1‑149 (MQ=255) aGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGAt < 2:12451/149‑1 (MQ=255) aGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGAt > 5:91469/1‑149 (MQ=255) cAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTAcc < 5:391553/149‑1 (MQ=255) cAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACATCGTCTGACCGTGAGGATTTTTTAATCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATACCGGCCAAGAAACGGGCGATGTTccgccgccg > 1:305167/1‑149 (MQ=255) cTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAgg > 6:111022/1‑148 (MQ=255) ggcCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg < 6:226740/110‑1 (MQ=255) ggcCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCgg > 5:226740/1‑110 (MQ=255) ccAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTc < 4:372457/149‑1 (MQ=255) cAACTACGACGTGATCGGGCAAGAACACGGTGCCACAGCGGCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcg < 8:229813/149‑1 (MQ=255) aCTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg < 4:348125/116‑1 (MQ=255) aCTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACgg > 3:348125/1‑116 (MQ=255) cTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcgac > 1:384862/1‑148 (MQ=255) cgacgTGATCTCGCAAGAACACGGTGCCACTGCGGCTGACCGTGAGTATGTTTGCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGAGTTCCCGTCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAt < 5:267794/148‑1 (MQ=255) acgTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGc > 8:38285/1‑100 (MQ=255) gATCTGGCAAGAACACGGTGCCACAGCGTCTGA‑CATGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcga < 6:156969/137‑1 (MQ=255) gATCTGGCAAGAACACGGTGCCACAGCGTCTGA‑CATGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTcga > 5:156969/1‑137 (MQ=255) | TCGAAGCGTTGAAATGCCTGGGCAGAACCTTGGGTGTGACACAGCTGTATGCCGTCAGCGACGCCTGCCGGCACCATCGCCATCCCTATTTTGGCAGCGACAAGGGCCAGGACCTGGCGGCCAACTACGACGTGATCTGGCAAGAACACGGTGCCACAGCGTCTGACCGTGAGGATTTTTTCAGCCTGCCATTAGCGCCGGCGCAACGGGCGGAAACTGACATCCCGGCCAAGAAACGGGCGATGTACCGCCGCCGCCAGGCATTGCTCGACGAT > NC_002947/3553929‑3554203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |