Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,638,663 T→G 9.3% L220R (CTG→CGG)  PP_3205 → fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,638,6630TG 9.3% 158.3 / 7.5 54L220R (CTG→CGG) PP_3205fumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base G (3/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGA  >  NC_002947/3638517‑3638810
                                                                                                                                                  |                                                                                                                                                   
tGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGTGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGc                                                                                                                                                   <  1:231633/149‑1 (MQ=255)
   cAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACg                                                                                                                                                >  7:246615/1‑149 (MQ=255)
    aGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGt                                                                                                                                               >  5:164069/1‑149 (MQ=255)
    aGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGt                                                                                                                                               >  2:349816/1‑149 (MQ=255)
    aGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACGTGCCGCGGCAAGt                                                                                                                                               >  7:227803/1‑149 (MQ=255)
          cGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACGTGCAGCGGCAAGTGCAc                                                                                                                                           <  8:227803/147‑1 (MQ=255)
          cGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACGTGCAGCGGCAAGTGCAc                                                                                                                                           <  1:63426/147‑1 (MQ=255)
             gCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGaa                                                                                                                                      >  3:108735/1‑149 (MQ=255)
                      ccGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTg                                                                                                                             <  8:327374/149‑1 (MQ=255)
                       cgcgGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTgg                                                                                                                            <  2:274883/149‑1 (MQ=255)
                           ggAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCg                                                                                                                        >  4:326442/1‑149 (MQ=255)
                                 gAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTCGTTCGGCCaac                                                                                                                  >  5:293742/1‑147 (MQ=255)
                                  aaGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATc                                                                                                                  <  6:192339/148‑1 (MQ=255)
                                    gACCGGCTTTGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATccc                                                                                                                >  3:274244/1‑148 (MQ=255)
                                    gACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATcccc                                                                                                               <  6:164069/149‑1 (MQ=255)
                                          cttcggcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATggcg                                                                                                         >  8:12766/1‑149 (MQ=255)
                                              ggcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGGACTGGAACGGCGAGTGGTTCGGCCATCCCCATGgcggcg                                                                                                      <  8:246615/148‑1 (MQ=255)
                                               gcttTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCgccgcc                                                                                                    <  7:12766/149‑1 (MQ=255)
                                                    ctcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCa                                                                                               >  8:184575/1‑149 (MQ=255)
                                                     tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATc                                                                                                                  <  2:97904/129‑1 (MQ=255)
                                                     tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATc                                                                                                                  >  1:97904/1‑129 (MQ=255)
                                                     tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCAc                                                                                              >  7:93542/1‑149 (MQ=255)
                                                     tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGCCGCCATgg                                                                                                >  2:390348/1‑146 (MQ=255)
                                                     tcCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGCCGCCATgg                                                                                                <  1:390348/147‑2 (MQ=255)
                                                           ccAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGt                                                                                        >  1:350812/1‑149 (MQ=255)
                                                                cccGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTcca                                                                                   <  7:184575/149‑1 (MQ=255)
                                                                  cGCCAGCAGTTTCGCGCCCGTTGCGGTCACACCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTccac                                                                                  >  8:254781/1‑148 (MQ=255)
                                                                      agcagTTTCGCGGCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGtt                                                                             <  8:138051/149‑1 (MQ=255)
                                                                           tttCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTg                                                                        >  1:249990/1‑149 (MQ=255)
                                                                           tttCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTg                                                                        <  3:326442/149‑1 (MQ=255)
                                                                                cgcCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCAt                                                                   >  7:348871/1‑149 (MQ=255)
                                                                                            tCACCCCGGATGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacg                                                       <  8:243754/149‑1 (MQ=255)
                                                                                                cccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGGACTTCGGTTTCCACCAGTTGATTGCCCATGCTGCGCTgacgcgac                                                   <  2:350812/149‑1 (MQ=255)
                                                                                                cccGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTgacgcgac                                                   >  6:74529/1‑149 (MQ=255)
                                                                                                   ggACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCt                                                >  6:264396/1‑149 (MQ=255)
                                                                                                   ggACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCAGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCt                                                >  1:235356/1‑149 (MQ=255)
                                                                                                      cGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGa                                             <  4:274244/149‑1 (MQ=255)
                                                                                                      cGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGa                                             <  2:249990/149‑1 (MQ=255)
                                                                                                         aCTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCCCCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTg                                          >  8:95683/1‑149 (MQ=255)
                                                                                                             ggCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCAGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCgg                                       <  2:235356/148‑1 (MQ=255)
                                                                                                              gCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGc                                     <  8:93542/149‑1 (MQ=255)
                                                                                                                     cTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTAc                              <  7:254781/149‑1 (MQ=255)
                                                                                                                     cTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTAc                              >  2:274998/1‑149 (MQ=255)
                                                                                                                          gcgcCGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGc                         <  1:349816/149‑1 (MQ=255)
                                                                                                                                ttccagTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGc                   >  2:345635/6‑149 (MQ=255)
                                                                                                                                 ccGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCa                  >  1:300937/1‑149 (MQ=255)
                                                                                                                                  cGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCAc                 >  6:364102/1‑149 (MQ=255)
                                                                                                                                   gggTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCAcc                <  5:364102/149‑1 (MQ=255)
                                                                                                                                    agTGCACGTGCAGCGGCAAGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCg               >  5:320329/2‑149 (MQ=255)
                                                                                                                                        cACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTc           <  3:193495/149‑1 (MQ=255)
                                                                                                                                         aCTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGATTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTc           >  6:191710/1‑148 (MQ=255)
                                                                                                                                              ccGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACACGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGc     >  8:128417/1‑149 (MQ=255)
                                                                                                                                               cGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCTACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGcc    <  2:374751/149‑1 (MQ=255)
                                                                                                                                                 cTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACACGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGa  <  7:128417/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGTCAGCCTGCGGGCCCTGGCACCGCGGGAAATGAAGACCGGCTTCGGCTTTCTCCAGGCCAAGCCCGCCAGCAGTTTCGCGCCCGTTGCGGTCACCCCGGACGAACTCGGCGAGGCCTGGCGCGACGGCCGGGTGCACTTGCCGCTGCACGTGCACTGGAACGGCGAGTGGTTCGGCCATCCCCATGGCGGCGCCATGCACTTCGGTTTCCACCAGTTGATTGCCCATGCGGCGCTGACGCGACGCTTGAGTGCGGGCACGCTACTGGGCTCCGGCACCGTGTCCAACGCCGA  >  NC_002947/3638517‑3638810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑