Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 268,746 G→C 7.1% intergenic (‑77/+23) PP_0216 ← / ← desA sensory box/GGDEF family protein/delta‑9 fatty acid desaturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947268,7460GC 7.1% 223.0 / 6.4 70intergenic (‑77/+23)PP_0216/desAsensory box/GGDEF family protein/delta‑9 fatty acid desaturase
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base C (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

GGGAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTGG  >  NC_002947/268599‑268889
                                                                                                                                                   |                                                                                                                                               
gggAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTggg                                                                                                                                                <  1:188865/149‑1 (MQ=255)
 ggAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAACGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTgggg                                                                                                                                               <  3:333044/149‑1 (MQ=255)
 ggAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTgggg                                                                                                                                               <  3:215661/149‑1 (MQ=255)
 ggAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACCTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTgggg                                                                                                                                               >  1:261341/1‑149 (MQ=255)
    aaaTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGc                                                                                                                                            <  7:375896/149‑1 (MQ=255)
      aTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGt                                                                                                                                          <  8:244438/149‑1 (MQ=255)
      aTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCCCAGCGGGGCGt                                                                                                                                          <  3:402784/149‑1 (MQ=255)
       tCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCCCAGCGGGGCGTg                                                                                                                                         <  7:28239/149‑1 (MQ=255)
       tCGTTGGAATGTGCTTGTCGATGTGCACGGAGCAGGGCTCAACGTGAACGCGTGCGGCCAGCATCCGGCCCGCCACGGAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTg                                                                                                                                         <  7:179627/149‑1 (MQ=255)
             gAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGGGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCGGGGGGGCGTGCCGATc                                                                                                                                   <  3:78053/149‑1 (MQ=255)
             gAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGAt                                                                                                                                    >  3:95384/1‑148 (MQ=255)
             caaTGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGTATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGAt                                                                                                                                    <  5:356638/147‑1 (MQ=255)
                tGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATc                                                                                                                                   <  8:246061/146‑1 (MQ=255)
                tGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCaag                                                                                                                                >  7:246061/1‑148 (MQ=255)
                     ttGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggcgg                                                                                                                           >  8:201835/1‑149 (MQ=255)
                     ttGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggcgg                                                                                                                           >  7:1994/1‑149 (MQ=255)
                     ttGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggcgg                                                                                                                           >  3:332761/1‑149 (MQ=255)
                       ggCCATGTGCACGGAGCAGGTCTCAACGAGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGCGCGAACTTCCGAATGGCACGCCCCGCTGGGGGGCGAGCCGATCAAcggcggc                                                                                                                          >  1:71979/1‑147 (MQ=255)
                        gCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCa                                                                                                                        <  2:250055/149‑1 (MQ=255)
                         ccATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAg                                                                                                                       <  1:253753/149‑1 (MQ=255)
                          cATGTGCACGGAGCAGGTCTCAACGTGAAAGCTTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAgg                                                                                                                      <  3:48450/149‑1 (MQ=255)
                          cATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAgg                                                                                                                      >  5:373835/1‑149 (MQ=255)
                           aTGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCg                                                                                                                                      >  4:105251/1‑132 (MQ=255)
                           aTGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCg                                                                                                                                      <  3:105251/132‑1 (MQ=255)
                                cACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCg                                                                                                                 <  5:166552/148‑1 (MQ=255)
                                 aCGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGt                                                                                                               >  4:11924/1‑149 (MQ=255)
                                 aCGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGt                                                                                                               >  4:143902/1‑149 (MQ=255)
                                     aGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCggg                                                                                                           <  4:95384/149‑1 (MQ=255)
                                      gCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGgc                                                                                                          <  4:150660/149‑1 (MQ=255)
                                       cAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGgcg                                                                                                         >  3:292256/1‑149 (MQ=255)
                                         ggTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCa                                                                                                       <  2:96885/149‑1 (MQ=255)
                                          gTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCg                                                                                                                 >  7:297225/1‑138 (MQ=255)
                                          gTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCg                                                                                                                 <  8:297225/138‑1 (MQ=255)
                                            ctcAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCAGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGc                                                                                                     >  1:300149/1‑148 (MQ=255)
                                               aaCGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAg                                                                                                  >  2:334436/1‑148 (MQ=255)
                                                      aaGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGTTATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCt                                                                                           <  2:261341/148‑1 (MQ=255)
                                                        gCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCtt                                                                                          >  7:245392/1‑147 (MQ=255)
                                                             cGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTtgt                                                                                   <  1:132790/149‑1 (MQ=255)
                                                             cGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTtgt                                                                                   >  2:132790/1‑149 (MQ=255)
                                                                ccAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGtgct                                                                                >  1:124630/1‑149 (MQ=255)
                                                                 cAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGtgctg                                                                               >  2:402952/1‑149 (MQ=255)
                                                                  aGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGtgctgc                                                                              <  8:245392/149‑1 (MQ=255)
                                                                   gCATCCGGCCCGCCACGCCGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGtgctgc                                                                              >  2:65495/1‑148 (MQ=255)
                                                                     aTCCGGCCCGCCACGCAGGCGATATTGCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTTGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGaa                                                                           <  7:201835/149‑1 (MQ=255)
                                                                     aTCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCg                                                                             >  7:116663/1‑147 (MQ=255)
                                                                     aTCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGaa                                                                           <  8:245868/149‑1 (MQ=255)
                                                                      tCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGGTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTTCTGCGAAg                                                                          >  5:245809/1‑149 (MQ=255)
                                                                         ggCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTcg                                                                       <  8:116663/149‑1 (MQ=255)
                                                                                   gCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCt                                                             <  8:177921/149‑1 (MQ=255)
                                                                                         gATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGc                                                       <  4:292256/149‑1 (MQ=255)
                                                                                         gATACTCCGGACTCGCGTGGAGCGGTGGGGGCGAAGTTCCGAATGGCCCGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGc                                                       <  4:332761/149‑1 (MQ=255)
                                                                                             ttCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCt                                                   >  5:418801/1‑149 (MQ=255)
                                                                                              tCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGa                                                                                                   >  3:183394/1‑100 (MQ=255)
                                                                                              tCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGa                                                                                                   <  4:183394/100‑1 (MQ=255)
                                                                                               ccGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTg                                                  <  6:373835/148‑1 (MQ=255)
                                                                                               ccGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTg                                                  <  5:139594/148‑1 (MQ=255)
                                                                                                         gTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCt                                        >  6:381170/1‑148 (MQ=255)
                                                                                                          tGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCa                                      <  1:65495/149‑1 (MQ=255)
                                                                                                          tGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCa                                      >  4:261684/1‑149 (MQ=255)
                                                                                                            gagaGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCAt                                     <  2:300149/148‑1 (MQ=255)
                                                                                                            gagaGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATg                                    >  5:105713/1‑149 (MQ=255)
                                                                                                                       gCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCtt                         >  5:182239/1‑149 (MQ=255)
                                                                                                                       gCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTAACCAGTCtt                         >  4:261722/1‑149 (MQ=255)
                                                                                                                                 gAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGc               <  8:302648/149‑1 (MQ=255)
                                                                                                                                   tcggCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAg                                                                  <  6:315571/94‑1 (MQ=39)
                                                                                                                                     ggCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAg            >  4:128154/1‑148 (MQ=255)
                                                                                                                                      gCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCa          >  5:376689/1‑149 (MQ=255)
                                                                                                                                      gCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCa          >  8:96603/1‑149 (MQ=255)
                                                                                                                                       cACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCAt         >  6:97355/1‑149 (MQ=255)
                                                                                                                                        aCGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCg                                            <  6:37566/113‑1 (MQ=255)
                                                                                                                                        aCGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCg                                            >  5:37566/1‑113 (MQ=255)
                                                                                                                                             ccGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAg            >  6:57516/1‑140 (MQ=255)
                                                                                                                                             ccGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAg            <  5:57516/140‑1 (MQ=255)
                                                                                                                                              cGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTgg  <  3:11924/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGGAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTGG  >  NC_002947/268599‑268889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑