Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,697,445 C→G 5.6% S15T (AGC→ACC)  PP_5595 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,697,4450CG 5.6% 228.2 / 2.2 70S15T (AGC→ACC) PP_5595hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base G (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGC  >  NC_002947/3697300‑3697591
                                                                                                                                                 |                                                                                                                                                   
gTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTgg                                                                                                                                                  >  4:153553/1‑149 (MQ=255)
   cTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACATGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGc                                                                                                                                                 >  7:265770/1‑147 (MQ=255)
   cTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCa                                                                                                                                               <  4:192236/149‑1 (MQ=255)
   cTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGATGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGc                                                                                                                                                 >  8:351716/1‑147 (MQ=255)
        tttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTg                                                                                                                                         >  4:145590/1‑149 (MQ=255)
        tttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCAGCGGCCAggtc                                                                                                                                           >  6:176165/1‑146 (MQ=255)
          tCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCAGCGGCCAggtc                                                                                                                                           <  5:176165/146‑3 (MQ=255)
           cGTTGCTGCATGAGCAGTTGATCGCTTGTTCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCAACCGGCGTCACGTCGTCTCTGTAGTAGCGAGTAAGCAGATTTCTAGCTTCGGCCCAGACCTGGCCGGTGGCGAGG‑CTGGc                                                                                                                                       <  2:93385/148‑1 (MQ=255)
            gTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCa                                                                                                                                      <  3:153553/148‑1 (MQ=255)
               gctgcATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAAcc                                                                                                                                   <  1:321710/148‑1 (MQ=255)
                   cATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatcat                                                                                                                              >  1:12688/1‑149 (MQ=255)
                   cATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatcat                                                                                                                              >  6:361575/1‑149 (MQ=255)
                    aTGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatcatc                                                                                                                             <  3:145590/149‑1 (MQ=255)
                      gACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatcatcat                                                                                                                           >  7:373345/1‑149 (MQ=255)
                              gATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAg                                                                                                                   >  7:312486/1‑149 (MQ=255)
                               aTCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGGCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAgg                                                                                                                  <  2:48766/149‑1 (MQ=255)
                                   cTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAaca                                                                                                              <  8:265770/149‑1 (MQ=255)
                                       ggcggGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCa                                                                                                          >  1:368816/1‑149 (MQ=255)
                                          gggTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGAAGGAACACTCAtat                                                                                                       >  6:73383/1‑147 (MQ=255)
                                            gTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTc                                                                                                     <  7:351716/149‑1 (MQ=255)
                                             tATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTcc                                                                                                    <  5:361575/149‑1 (MQ=255)
                                              aTGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCa                                                                                                   >  7:161460/1‑149 (MQ=255)
                                                gTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCAc                                                                                                  <  8:161460/148‑1 (MQ=255)
                                                    ctgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTc                                                                                               >  5:26019/1‑147 (MQ=255)
                                                    ctgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTc                                                                                               >  2:129070/1‑147 (MQ=255)
                                                    ctgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTc                                                                                               >  1:244718/1‑147 (MQ=255)
                                                       tagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                           <  2:244718/148‑1 (MQ=255)
                                                       tagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                           <  5:136106/148‑1 (MQ=255)
                                                       tagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAg                                                                                            <  2:368816/147‑1 (MQ=255)
                                                             taACAGCTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACaa                                                                                     <  8:328926/148‑1 (MQ=255)
                                                                   gtgtGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                           >  1:90823/1‑136 (MQ=255)
                                                                   gtgtGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                           <  2:90823/136‑1 (MQ=255)
                                                                      actcGCTCCTCCAGCGACGCCGTGACGCCGGTGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCaa                                                                                                                        <  7:364123/101‑1 (MQ=21)
                                                                              cACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCt                                                                     >  1:388778/1‑147 (MQ=255)
                                                                                  agCGGCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAg                                                               <  1:149900/148‑1 (MQ=255)
                                                                                      tCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACGTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCtt                                                           <  3:143674/149‑1 (MQ=255)
                                                                                      tCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCtt                                                           >  3:392667/1‑149 (MQ=255)
                                                                                         cGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTc                                                        <  2:388778/149‑1 (MQ=255)
                                                                                         cGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTc                                                        <  8:229563/149‑1 (MQ=255)
                                                                                                    gaggagCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCa                                             >  8:304834/1‑149 (MQ=255)
                                                                                                            aGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTg                                     <  7:304834/149‑1 (MQ=255)
                                                                                                             gTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTgg                                    <  1:129070/149‑1 (MQ=255)
                                                                                                                aGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGgcg                                  <  2:220848/148‑1 (MQ=255)
                                                                                                                  cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGcgcgg                               >  7:224897/1‑148 (MQ=255)
                                                                                                                  cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGt                               >  6:48059/1‑149 (MQ=255)
                                                                                                                  cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGt                               <  5:48059/149‑1 (MQ=255)
                                                                                                                  cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGt                               <  2:12688/149‑1 (MQ=255)
                                                                                                                      ttGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                      >  7:246301/1‑138 (MQ=255)
                                                                                                                      ttGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                      <  8:246301/138‑1 (MQ=255)
                                                                                                                         cAAGCTTCGTCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                        <  5:73383/149‑1 (MQ=255)
                                                                                                                         cAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCCCAAGGCCCTCGTCAGGGCTGCGCAGGCTGGTCGCGCCGGCGCATGGGTTTGGGGCGTTTTTccc                        >  7:152488/1‑148 (MQ=255)
                                                                                                                          aaGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCg                                                                             <  7:338562/95‑1 (MQ=255)
                                                                                                                          aaGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCg                                                                             >  8:338562/1‑95 (MQ=255)
                                                                                                                          aaGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAg                       <  8:312486/149‑1 (MQ=255)
                                                                                                                              ttCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGAt                    >  4:378991/1‑148 (MQ=255)
                                                                                                                                  gCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggt               <  8:373345/149‑1 (MQ=255)
                                                                                                                                  gCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggt               <  3:61885/149‑1 (MQ=255)
                                                                                                                                  gCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCagg                >  5:130116/1‑148 (MQ=255)
                                                                                                                                    ccAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtag             <  6:26019/149‑1 (MQ=255)
                                                                                                                                     cAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCGGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtagg            >  7:214372/1‑149 (MQ=255)
                                                                                                                                      cagccTGGCCAGCGGCCAGGTCTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtagg            >  5:350027/4‑149 (MQ=255)
                                                                                                                                        aCCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGACCACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTca         >  3:323410/1‑149 (MQ=255)
                                                                                                                                         ccTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTcac        >  7:146255/1‑149 (MQ=255)
                                                                                                                                          cTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGgcgc                                              >  5:173758/1‑110 (MQ=255)
                                                                                                                                          cTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGgcgc                                              <  6:173758/110‑1 (MQ=255)
                                                                                                                                           tGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTcacca      >  8:385538/1‑149 (MQ=255)
                                                                                                                                             gCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACt    >  6:71221/1‑149 (MQ=255)
                                                                                                                                               cGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                        <  3:75800/127‑1 (MQ=255)
                                                                                                                                               cGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                        >  4:75800/1‑127 (MQ=255)
                                                                                                                                               cGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGc  >  6:345596/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGC  >  NC_002947/3697300‑3697591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑