Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,766,152 A→C 5.8% R161R (CGA→CGC) ‡ PP_5599 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,766,1520AC 5.8% 273.4 / 6.6 86R161R (CGA→CGC) ‡PP_5599LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (41/40);  new base C (2/3);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AGCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCAG  >  NC_002947/3766006‑3766299
                                                                                                                                                  |                                                                                                                                                   
aGCATTGCCCCGCAACTGAGGCTACGCATCACGGCATGTGACTCTCGCAGTCTTTACGAAAAAGTAGGCTTCGGAGTGCTCGATGCGGGATTCTTGGTATGTCCGGCGTTCAGCTTCGCCAAAATCATTCGTATACAGGTTCTTCgata                                                                                                                                                   <  4:388865/149‑3 (MQ=255)
 gCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGagaa                                                                                                                                                  <  5:171749/149‑1 (MQ=255)
       ccccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAAccc                                                                                                                                            <  4:266728/149‑1 (MQ=255)
       ccccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAAccc                                                                                                                                            <  2:72819/149‑1 (MQ=255)
         ccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCTCGAAGAAcctgt                                                                                                                                          <  6:97724/149‑4 (MQ=255)
         ccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCt                                                                                                                                          >  5:152935/1‑149 (MQ=255)
           gCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGa                                                                                                                                        <  7:119282/149‑1 (MQ=255)
                  aGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCttt                                                                                                                                  <  2:20234/148‑1 (MQ=255)
                      tACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGAc                                                                                                                              >  5:78835/1‑148 (MQ=255)
                       aCGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGAcc                                                                                                                             >  6:328430/1‑148 (MQ=255)
                       aCGAATCACGCCAGTGGACTCGCGCAGTCTTTACGAAAAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGAcc                                                                                                                             <  4:134791/148‑1 (MQ=255)
                         gCATCACGCCAGGGGACTTGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCaaa                                                                                                                          <  8:359141/149‑1 (MQ=255)
                           aTCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGa                                                                                                                        >  8:184121/1‑149 (MQ=255)
                           aTCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCATCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGa                                                                                                                        >  1:178784/1‑149 (MQ=255)
                            tCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGAc                                                                                                                       >  3:226527/1‑149 (MQ=255)
                             cACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGAcc                                                                                                                      >  7:80720/1‑149 (MQ=255)
                                gCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCg                                                                                                                   <  6:78835/149‑1 (MQ=255)
                                  cAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGtt                                                                                                                 <  4:234227/149‑1 (MQ=255)
                                  cAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGtt                                                                                                                 <  6:211257/149‑1 (MQ=255)
                                    ggggACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGa                                                                                                               <  1:253027/149‑1 (MQ=255)
                                          tCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAGAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAgcg                                                                                                         >  4:358726/1‑149 (MQ=255)
                                          tCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAgcg                                                                                                         >  3:301572/1‑149 (MQ=255)
                                            gcgcAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGCCCCCGTTGCCAAgcgc                                                                                                        >  8:316768/1‑148 (MQ=255)
                                            gcgcAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGtt                                                                                                                 >  1:397433/1‑139 (MQ=255)
                                            gcgcAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGtt                                                                                                                 <  2:397433/139‑1 (MQ=255)
                                            gcgcAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAgcgc                                                                                                        >  1:74064/1‑148 (MQ=255)
                                              gcAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGAc                                                                                                     >  8:97612/1‑149 (MQ=255)
                                                  tCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATcc                                                                                                 >  1:297327/1‑149 (MQ=255)
                                                    tttACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAGCCCCTGATGCTTTAGACCAAAGACCCCGTTGACAAGCGCGAGATCCGc                                                                                               >  6:85211/1‑149 (MQ=255)
                                                    tttACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCGAAGACCCCGTTGACAAGCGCGACATCCg                                                                                                >  5:228547/1‑148 (MQ=255)
                                                    tttACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGc                                                                                               >  6:185959/1‑149 (MQ=255)
                                                       aCGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTc                                                                                             >  5:411481/1‑148 (MQ=255)
                                                        cGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTcga                                                                                           <  2:297327/149‑1 (MQ=255)
                                                         gAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTcgac                                                                                          >  6:152739/1‑149 (MQ=255)
                                                             aaGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGAtt                                                                                      <  4:226527/149‑1 (MQ=255)
                                                               gTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCt                                                                                    >  3:131238/1‑149 (MQ=255)
                                                                  gCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAGAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTgg                                                                                 <  3:358726/149‑1 (MQ=255)
                                                                  gCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTgg                                                                                 >  3:235294/1‑149 (MQ=255)
                                                                   ccTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGCTGACAAGCGCGACATCCGCTCGACGATTCTTGGt                                                                                >  3:45113/1‑149 (MQ=255)
                                                                       cGGAGAGCTCTATGCGGTAGTCATTGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGaa                                                                            <  6:411481/149‑1 (MQ=255)
                                                                       cGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACaa                                                                                                            >  4:36141/1‑117 (MQ=255)
                                                                        ggAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTTGaaa                                                                           >  1:385728/1‑149 (MQ=255)
                                                                        ggAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGaaa                                                                           <  8:287517/149‑1 (MQ=255)
                                                                        ggAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGCTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGaaa                                                                           <  4:45113/149‑1 (MQ=255)
                                                                         gagaGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAAc                                                                          >  6:404745/1‑149 (MQ=255)
                                                                              cTCGATGCGGGAGTCATGGTATGTCCGCCCTACAGCGTCCCCAAAAGCATACGAATACAGGTTCATCCAGAAGAACCCCTGATGCTTTTGACCATACACCCCCTTCACAAGCGCGACATCCCCACGACGATACTTGGTCGAAACCTGAt                                                                     >  2:182865/1‑149 (MQ=255)
                                                                                   tGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCGAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGat                                                                <  6:228547/149‑1 (MQ=255)
                                                                                   tGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGat                                                                <  4:235294/149‑1 (MQ=255)
                                                                                   tGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGat                                                                <  6:152935/149‑1 (MQ=255)
                                                                                   tGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGat                                                                <  2:74064/149‑1 (MQ=255)
                                                                                    gCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGcgcg                                                                                                       >  6:382180/1‑109 (MQ=255)
                                                                                    gCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGcgcg                                                                                                       <  5:382180/109‑1 (MQ=255)
                                                                                        gAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGAc                                                           <  4:131238/149‑1 (MQ=255)
                                                                                          gTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTc                                                         <  5:185959/149‑1 (MQ=255)
                                                                                             aTGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTAATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTcaca                                                      >  6:257090/1‑149 (MQ=255)
                                                                                               ggTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATg                                                    >  5:263519/1‑149 (MQ=255)
                                                                                               ggTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCATTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATg                                                    >  1:125201/1‑149 (MQ=255)
                                                                                                gTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGc                                                   >  1:337255/1‑149 (MQ=255)
                                                                                                     tCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTggg                                              >  4:229118/1‑149 (MQ=255)
                                                                                                     tCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTAATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTggg                                              <  5:257090/149‑1 (MQ=255)
                                                                                                         cccTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTgg                                          <  7:184121/149‑1 (MQ=255)
                                                                                                         cccTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTgg                                          <  4:301572/149‑1 (MQ=255)
                                                                                                         cccTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACACACATGCCTGGGGTgg                                          <  2:356012/149‑1 (MQ=255)
                                                                                                           cTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGc                                        <  7:77647/149‑1 (MQ=255)
                                                                                                           cTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGc                                        <  3:229118/149‑1 (MQ=255)
                                                                                                            ttCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCa                                       <  8:80720/149‑1 (MQ=255)
                                                                                                             tCAGCGTTCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTg                                                                                  <  1:238008/105‑1 (MQ=255)
                                                                                                             tCAGCGTACCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTg                                                                                  >  2:238008/1‑105 (MQ=255)
                                                                                                                 cGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTg                                  >  5:190041/1‑149 (MQ=255)
                                                                                                                  gTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCa                                       <  4:350117/143‑1 (MQ=255)
                                                                                                                  gTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCa                                       >  3:350117/1‑143 (MQ=255)
                                                                                                                       cAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCcag                             >  1:264547/1‑148 (MQ=255)
                                                                                                                       cAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCcag                             >  8:162375/1‑148 (MQ=255)
                                                                                                                              aTTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGGCATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACtt                     >  6:163058/1‑149 (MQ=255)
                                                                                                                                  gTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaat                 <  5:328430/149‑1 (MQ=255)
                                                                                                                                  gTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaat                 <  1:113431/149‑1 (MQ=255)
                                                                                                                                  gTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaa                  <  5:85211/148‑1 (MQ=255)
                                                                                                                                   atcaggggTTCTTCTCGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaat                 >  4:324804/7‑148 (MQ=255)
                                                                                                                                   atcaggggTTCTTCTCGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaat                 <  3:324804/142‑1 (MQ=255)
                                                                                                                                     taCAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTTGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaatga               <  2:385728/148‑1 (MQ=255)
                                                                                                                                     taCAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaatga               <  2:178784/148‑1 (MQ=255)
                                                                                                                                     taCAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGATCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGTTGGGCAGATTGACCAGCAGTACTTGaatga               >  8:310436/1‑148 (MQ=255)
                                                                                                                                       cAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaatgacaa            >  7:271669/1‑149 (MQ=255)
                                                                                                                                        gggTTCTTCTCGAAGAACCTCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCa                                       <  7:12720/120‑1 (MQ=255)
                                                                                                                                        gggTTCTTCTCGAAGAACCTCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCa                                       >  8:12720/2‑121 (MQ=255)
                                                                                                                                          gTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaatgacaatga         >  4:61816/1‑149 (MQ=255)
                                                                                                                                           ttcttcGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaatgacaatgac        <  7:162375/149‑1 (MQ=255)
                                                                                                                                             cttcGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGAcc       <  6:190041/148‑1 (MQ=255)
                                                                                                                                                 gagaAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCa   <  2:337255/148‑1 (MQ=255)
                                                                                                                                                 gagaAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCAg  <  3:320589/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCAG  >  NC_002947/3766006‑3766299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑