Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,775,321 C→A 8.0% M52I (ATG→ATT) ‡ PP_3336 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,775,3210CA 8.0% 279.2 / 10.7 87M52I (ATG→ATT) ‡PP_3336hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/40);  new base A (3/4);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

CTTGGGCGCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTC  >  NC_002947/3775176‑3775469
                                                                                                                                                 |                                                                                                                                                    
cTTGGGCGCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATg                                                                                                                                                   >  5:301978/1‑149 (MQ=255)
   gggCGCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATgg                                                                                                                                                  <  8:170239/147‑1 (MQ=255)
       gcagcagcaAAGCCCGGGGGCGGACGTCTTCTGGCTAAGTTTGCCGTCCTTGTCCACCATGGCCGGCTGTACGCCGCCCCACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGACCAGGGTACTGGCCATGGGCAATg                                                                                                                                            >  4:81911/1‑149 (MQ=255)
        cagcagcaATGCCGGGGGGCTGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGc                                                                                                                                           <  6:283748/149‑1 (MQ=255)
          gcagcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCa                                                                                                                                         <  6:330919/149‑1 (MQ=255)
            agcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAg                                                                                                                                        >  2:402684/1‑148 (MQ=255)
              caATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTAc                                                                                                                                     <  8:80435/149‑1 (MQ=255)
              caATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTAc                                                                                                                                     <  4:14671/149‑1 (MQ=255)
               aaTGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCATTGCCCATGGCCAGTAcc                                                                                                                                    <  4:4268/149‑1 (MQ=255)
               aaTGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCATTGCCCATGGCCAGTAcc                                                                                                                                    <  4:9788/149‑1 (MQ=255)
                   cccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAAGGTACTGGCATTGCCCATGGCCAGTACCCTgg                                                                                                                                <  6:375867/149‑2 (MQ=38)
                    ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                               >  2:210257/1‑149 (MQ=255)
                    ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                               >  6:314995/1‑149 (MQ=255)
                     cGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGagc                                                                                                                              <  7:122364/149‑1 (MQ=255)
                        gggCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGagcagc                                                                                                                           <  1:210257/149‑1 (MQ=255)
                         ggCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAgcagca                                                                                                                          <  2:398435/149‑1 (MQ=255)
                           cGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATg                                                                                                                        <  6:170208/149‑1 (MQ=255)
                             gACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATgg                                                                                                                       >  6:244344/1‑148 (MQ=255)
                             gACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGc                                                                                                                      <  5:118297/149‑1 (MQ=255)
                             gACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGc                                                                                                                      <  8:266818/149‑1 (MQ=255)
                                gTCTTCGGGCTAAGTTTGCCGTCCTTGTCCCTCATGGCAGGCTGTACGCCGCCCGACGAGCAATAACTGCCCTCCAGCAACTGCGCCAGGCTCAGGTCTCCCAGGGTACTGGCCATGTGCAATGCCAGTACCTTGAGCAGCATGGCAag                                                                                                                   <  8:14956/149‑1 (MQ=255)
                                gTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAag                                                                                                                   <  3:219553/149‑1 (MQ=255)
                                   ttCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagcag                                                                                                                >  5:67712/1‑149 (MQ=255)
                                     cGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagca                                                                                                                 <  1:204815/146‑1 (MQ=255)
                                       ggCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGcgcc                                                                                                            >  4:398898/1‑149 (MQ=255)
                                          tAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGcgccgc                                                                                                          >  1:273415/1‑148 (MQ=255)
                                             gTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGAcc                                                                                                      >  3:292407/1‑149 (MQ=255)
                                               ttGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCACGCAGCGCCGCGccc                                                                                                      >  8:106004/1‑147 (MQ=255)
                                                 gCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCAAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCaga                                                                                                  >  4:332262/1‑149 (MQ=255)
                                                  ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCagag                                                                                                 >  7:164277/1‑149 (MQ=255)
                                                  ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCagag                                                                                                 <  3:290885/149‑1 (MQ=255)
                                                  ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCaga                                                                                                  <  6:301978/148‑1 (MQ=255)
                                                   cGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAgg                                                                                                >  4:176083/1‑149 (MQ=255)
                                                     tCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGtt                                                                                              <  4:292407/149‑1 (MQ=255)
                                                     tCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGt                                                                                               >  2:8280/1‑148 (MQ=255)
                                                          gTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATc                                                                                           <  6:380684/147‑1 (MQ=255)
                                                            ccAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAg                                                                                       >  5:192752/1‑149 (MQ=255)
                                                             cAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAg                                                                                       <  5:244344/148‑1 (MQ=255)
                                                              aGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGgt                                                                                     >  5:287124/1‑149 (MQ=255)
                                                                cATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGgtgc                                                                                   >  5:260533/1‑149 (MQ=255)
                                                                    gCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGgtgcgtgc                                                                               <  2:273415/149‑1 (MQ=255)
                                                                     ccGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGtgcgtgcg                                                                              >  4:232009/1‑149 (MQ=255)
                                                                      cGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCAt                                                                                                                         <  1:2618/105‑1 (MQ=255)
                                                                      cGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCAt                                                                                                                         >  2:2618/1‑105 (MQ=255)
                                                                         cTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTgcgcg                                                                            >  5:381792/1‑147 (MQ=255)
                                                                               gccgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCg                                                                     >  5:147926/1‑148 (MQ=255)
                                                                               gccgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCg                                                                     >  6:204232/1‑148 (MQ=255)
                                                                                 cgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCaa                                                                  >  1:151993/1‑149 (MQ=255)
                                                                                    ccGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATgg                                                               <  6:287124/149‑1 (MQ=255)
                                                                                       acgaGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGgcgc                                                            <  6:307552/149‑1 (MQ=255)
                                                                                          aGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATg                                                         <  2:151993/149‑1 (MQ=255)
                                                                                            cAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGtt                                                       >  7:248442/1‑149 (MQ=255)
                                                                                              atatCTGCCCGGCAGCATCTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCTATGCCAGTACCTTGAGCAGCATGGCAAGCTGCGCCGCGACCCAGAGGTTATCCGAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCa                                                     <  3:81911/149‑1 (MQ=255)
                                                                                              aTAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCa                                                     <  1:402684/149‑1 (MQ=255)
                                                                                               tAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAgg                                                                                                >  1:376217/1‑105 (MQ=255)
                                                                                               tAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAgg                                                                                                <  2:376217/105‑1 (MQ=255)
                                                                                                      ccGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGCGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGc                                             >  2:276068/1‑149 (MQ=255)
                                                                                                        gCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGc                                             >  8:30576/1‑147 (MQ=255)
                                                                                                        gCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCa                                           <  5:314995/149‑1 (MQ=255)
                                                                                                          cAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGCGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAgg                                         <  1:276068/149‑1 (MQ=255)
                                                                                                              aaCTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAgc                                     >  5:412747/1‑149 (MQ=255)
                                                                                                                    gcCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGc                               >  8:375360/1‑149 (MQ=255)
                                                                                                                        ggCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGTGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                           <  1:310702/149‑1 (MQ=255)
                                                                                                                        ggCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                           <  7:27164/149‑1 (MQ=255)
                                                                                                                         gCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                            >  2:128614/1‑147 (MQ=255)
                                                                                                                         gCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGga                          <  6:192752/149‑1 (MQ=255)
                                                                                                                           tCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                            >  5:161484/1‑145 (MQ=255)
                                                                                                                           tCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                            >  3:173877/1‑145 (MQ=255)
                                                                                                                            cAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                           >  6:88983/1‑145 (MQ=255)
                                                                                                                            cAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                           <  5:88983/145‑1 (MQ=255)
                                                                                                                             aGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGgaga                        <  7:333003/147‑1 (MQ=255)
                                                                                                                              ggTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                     <  7:30576/149‑1 (MQ=255)
                                                                                                                               gTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                     >  5:41425/1‑148 (MQ=255)
                                                                                                                                   cccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGAtt                      <  3:66463/143‑1 (MQ=255)
                                                                                                                                   cccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGAtt                      >  4:66463/1‑143 (MQ=255)
                                                                                                                                   cccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAg                <  3:398898/149‑1 (MQ=255)
                                                                                                                                    ccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAgg               >  6:78921/1‑149 (MQ=255)
                                                                                                                                    ccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAgg               >  7:321453/1‑149 (MQ=255)
                                                                                                                                      aGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTc             <  6:41425/149‑1 (MQ=255)
                                                                                                                                        ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCggg                                >  2:295608/1‑128 (MQ=255)
                                                                                                                                        ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCggg                                <  1:295608/128‑1 (MQ=255)
                                                                                                                                        ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt           >  5:84069/1‑149 (MQ=255)
                                                                                                                                        ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt           >  5:342898/1‑149 (MQ=255)
                                                                                                                                          tACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt           >  2:270057/1‑147 (MQ=255)
                                                                                                                                          tACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGa         <  6:161484/149‑1 (MQ=255)
                                                                                                                                           aCTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCAGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAg        >  8:316185/1‑149 (MQ=255)
                                                                                                                                              ggCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGc     <  6:412747/149‑1 (MQ=255)
                                                                                                                                                 cATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtc  >  6:416527/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTTGGGCGCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTC  >  NC_002947/3775176‑3775469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑