Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,775,330 T→G 7.5% A49A (GCA→GCC)  PP_3336 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,775,3300TG 7.5% 290.1 / 13.0 93A49A (GCA→GCC) PP_3336hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (43/43);  new base G (3/4);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

GCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAAC  >  NC_002947/3775183‑3775477
                                                                                                                                                   |                                                                                                                                                   
gcagcagcaAAGCCCGGGGGCGGACGTCTTCTGGCTAAGTTTGCCGTCCTTGTCCACCATGGCCGGCTGTACGCCGCCCCACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGACCAGGGTACTGGCCATGGGCAATg                                                                                                                                                    >  4:81911/1‑149 (MQ=255)
 cagcagcaATGCCGGGGGGCTGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGc                                                                                                                                                   <  6:283748/149‑1 (MQ=255)
   gcagcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCa                                                                                                                                                 <  6:330919/149‑1 (MQ=255)
     agcaATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAg                                                                                                                                                >  2:402684/1‑148 (MQ=255)
       caATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTAc                                                                                                                                             <  8:80435/149‑1 (MQ=255)
       caATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTAc                                                                                                                                             <  4:14671/149‑1 (MQ=255)
        aaTGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCATTGCCCATGGCCAGTAcc                                                                                                                                            <  4:4268/149‑1 (MQ=255)
        aaTGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCATTGCCCATGGCCAGTAcc                                                                                                                                            <  4:9788/149‑1 (MQ=255)
            cccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAAGGTACTGGCATTGCCCATGGCCAGTACCCTgg                                                                                                                                        <  6:375867/149‑2 (MQ=38)
             ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                                       >  6:314995/1‑149 (MQ=255)
             ccGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGag                                                                                                                                       >  2:210257/1‑149 (MQ=255)
              cGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGagc                                                                                                                                      <  7:122364/149‑1 (MQ=255)
                 gggCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGagcagc                                                                                                                                   <  1:210257/149‑1 (MQ=255)
                  ggCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAgcagca                                                                                                                                  <  2:398435/149‑1 (MQ=255)
                    cGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATg                                                                                                                                <  6:170208/149‑1 (MQ=255)
                      gACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATgg                                                                                                                               >  6:244344/1‑148 (MQ=255)
                      gACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGc                                                                                                                              <  5:118297/149‑1 (MQ=255)
                      gACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGc                                                                                                                              <  8:266818/149‑1 (MQ=255)
                         gTCTTCGGGCTAAGTTTGCCGTCCTTGTCCCTCATGGCAGGCTGTACGCCGCCCGACGAGCAATAACTGCCCTCCAGCAACTGCGCCAGGCTCAGGTCTCCCAGGGTACTGGCCATGTGCAATGCCAGTACCTTGAGCAGCATGGCAag                                                                                                                           <  8:14956/149‑1 (MQ=255)
                         gTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAag                                                                                                                           <  3:219553/149‑1 (MQ=255)
                            ttCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagcag                                                                                                                        >  5:67712/1‑149 (MQ=255)
                              cGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAagca                                                                                                                         <  1:204815/146‑1 (MQ=255)
                                ggCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGcgcc                                                                                                                    >  4:398898/1‑149 (MQ=255)
                                   tAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGcgccgc                                                                                                                  >  1:273415/1‑148 (MQ=255)
                                      gTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGAcc                                                                                                              >  3:292407/1‑149 (MQ=255)
                                        ttGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCACGCAGCGCCGCGccc                                                                                                              >  8:106004/1‑147 (MQ=255)
                                          gCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCAAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCaga                                                                                                          >  4:332262/1‑149 (MQ=255)
                                           ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCagag                                                                                                         <  3:290885/149‑1 (MQ=255)
                                           ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCagag                                                                                                         >  7:164277/1‑149 (MQ=255)
                                           ccGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCaga                                                                                                          <  6:301978/148‑1 (MQ=255)
                                            cGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAgg                                                                                                        >  4:176083/1‑149 (MQ=255)
                                              tCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGtt                                                                                                      <  4:292407/149‑1 (MQ=255)
                                              tCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGt                                                                                                       >  2:8280/1‑148 (MQ=255)
                                                   gTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATc                                                                                                   <  6:380684/147‑1 (MQ=255)
                                                     ccAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAg                                                                                               >  5:192752/1‑149 (MQ=255)
                                                      cAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAg                                                                                               <  5:244344/148‑1 (MQ=255)
                                                       aGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGgt                                                                                             >  5:287124/1‑149 (MQ=255)
                                                         cATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGgtgc                                                                                           >  5:260533/1‑149 (MQ=255)
                                                             gCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGgtgcgtgc                                                                                       <  2:273415/149‑1 (MQ=255)
                                                              ccGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGtgcgtgcg                                                                                      >  4:232009/1‑149 (MQ=255)
                                                               cGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCAt                                                                                                                                 <  1:2618/105‑1 (MQ=255)
                                                               cGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCAt                                                                                                                                 >  2:2618/1‑105 (MQ=255)
                                                                  cTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTgcgcg                                                                                    >  5:381792/1‑147 (MQ=255)
                                                                        gccgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCg                                                                             >  5:147926/1‑148 (MQ=255)
                                                                        gccgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCg                                                                             >  6:204232/1‑148 (MQ=255)
                                                                          cgccCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCaa                                                                          >  1:151993/1‑149 (MQ=255)
                                                                             ccGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATgg                                                                       <  6:287124/149‑1 (MQ=255)
                                                                                acgaGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGgcgc                                                                    <  6:307552/149‑1 (MQ=255)
                                                                                   aGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATg                                                                 <  2:151993/149‑1 (MQ=255)
                                                                                     cAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGtt                                                               >  7:248442/1‑149 (MQ=255)
                                                                                       atatCTGCCCGGCAGCATCTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCTATGCCAGTACCTTGAGCAGCATGGCAAGCTGCGCCGCGACCCAGAGGTTATCCGAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCa                                                             <  3:81911/149‑1 (MQ=255)
                                                                                       aTAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCa                                                             <  1:402684/149‑1 (MQ=255)
                                                                                        tAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAgg                                                                                                        <  2:376217/105‑1 (MQ=255)
                                                                                        tAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAgg                                                                                                        >  1:376217/1‑105 (MQ=255)
                                                                                               ccGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGCGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGc                                                     >  2:276068/1‑149 (MQ=255)
                                                                                                 gCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGc                                                     >  8:30576/1‑147 (MQ=255)
                                                                                                 gCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCa                                                   <  5:314995/149‑1 (MQ=255)
                                                                                                   cAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGCGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAgg                                                 <  1:276068/149‑1 (MQ=255)
                                                                                                       aaCTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAgc                                             >  5:412747/1‑149 (MQ=255)
                                                                                                             gcCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGc                                       >  8:375360/1‑149 (MQ=255)
                                                                                                                 ggCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGTGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                                   <  1:310702/149‑1 (MQ=255)
                                                                                                                 ggCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                                   <  7:27164/149‑1 (MQ=255)
                                                                                                                  gCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                                    >  2:128614/1‑147 (MQ=255)
                                                                                                                  gCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGga                                  <  6:192752/149‑1 (MQ=255)
                                                                                                                    tCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                                    >  3:173877/1‑145 (MQ=255)
                                                                                                                    tCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTg                                    >  5:161484/1‑145 (MQ=255)
                                                                                                                     cAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                                   >  6:88983/1‑145 (MQ=255)
                                                                                                                     cAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTgg                                   <  5:88983/145‑1 (MQ=255)
                                                                                                                      aGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGgaga                                <  7:333003/147‑1 (MQ=255)
                                                                                                                       ggTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                             <  7:30576/149‑1 (MQ=255)
                                                                                                                        gTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTc                             >  5:41425/1‑148 (MQ=255)
                                                                                                                            cccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGAtt                              >  4:66463/1‑143 (MQ=255)
                                                                                                                            cccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGAtt                              <  3:66463/143‑1 (MQ=255)
                                                                                                                            cccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAg                        <  3:398898/149‑1 (MQ=255)
                                                                                                                             ccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAgg                       >  6:78921/1‑149 (MQ=255)
                                                                                                                             ccAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAgg                       >  7:321453/1‑149 (MQ=255)
                                                                                                                               aGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTc                     <  6:41425/149‑1 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCggg                                        <  1:295608/128‑1 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCggg                                        >  2:295608/1‑128 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt                   >  5:342898/1‑149 (MQ=255)
                                                                                                                                 ggTACTGGCATTGCCCATGGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt                   >  5:84069/1‑149 (MQ=255)
                                                                                                                                   tACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCAt                   >  2:270057/1‑147 (MQ=255)
                                                                                                                                   tACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGa                 <  6:161484/149‑1 (MQ=255)
                                                                                                                                    aCTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCAGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAg                >  8:316185/1‑149 (MQ=255)
                                                                                                                                       ggCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGc             <  6:412747/149‑1 (MQ=255)
                                                                                                                                          cATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtc          >  6:416527/1‑149 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtct         <  6:381792/149‑1 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtct         >  6:391282/1‑149 (MQ=255)
                                                                                                                                           aTGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTtct         >  4:226778/1‑149 (MQ=255)
                                                                                                                                                cAATGCCAGTACCTTGAGCAGCATGGCACGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCTCGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTcc     >  7:101705/1‑148 (MQ=255)
                                                                                                                                                 aaTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCaa   <  8:248442/149‑1 (MQ=255)
                                                                                                                                                 aaTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCaa   <  6:147926/149‑1 (MQ=255)
                                                                                                                                                  aTGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAac  <  3:176083/149‑1 (MQ=255)
                                                                                                                                                   tGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAac  >  6:253648/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAGCAGCAATGCCCGGGGGCGGACGTCTTCGGGCTAAGTTTGCCGTCCTTGTCCAGCATGGCCGGCTGTACGCCGCCCGACGAGCAATAACTGCCCGCCAGCAACTGCGCCAGGCTCAGGTCGCCCAGGGTACTGGCCATGGGCAATGCCAGTACCTTGAGCAGCATGGCAAGCAGCGCCGCGACCCAGAGGTTATCCCAGGTGCGTGCGCGCAGGCCGCAATGGCGCATGTTCACGCCTCGCCAGGTAGCGCGGGCGTGGAGATTCGACAGGTCATGAGGGCTTCTCTCCAAC  >  NC_002947/3775183‑3775477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑