| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,853,118 | T→A | 28.3% | I169N (ATT→AAT) | alkB → | alpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,853,118 | 0 | T | A | 28.3% | 43.1 / 12.0 | 30 | I169N (ATT→AAT) | alkB | alpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (19/2); new base A (5/3); total (24/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAACCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCTGA > NC_002947/3852971‑3853260 | tCAACCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAtt > 1:354144/1‑149 (MQ=255) aCCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAt < 8:36404/145‑1 (MQ=255) aCCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAt > 7:36404/1‑145 (MQ=255) ccTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGAGcc < 2:52011/149‑1 (MQ=255) cTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCa > 7:315756/1‑149 (MQ=255) tGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCAc > 4:68826/1‑149 (MQ=255) tGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGAGCCAc > 7:170508/1‑149 (MQ=255) gggCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGc > 1:203135/1‑148 (MQ=255) cACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGAtg > 8:316600/1‑149 (MQ=255) gcCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTg > 1:150093/1‑149 (MQ=255) gAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCt > 7:164340/1‑149 (MQ=255) aGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGAt > 1:244763/1‑138 (MQ=255) ccTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTggg > 3:223866/1‑149 (MQ=255) cTGCATCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGTGCCACGGCGATGTGCTGGTCTgggg > 6:60192/1‑149 (MQ=255) cTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTgggg > 7:217030/1‑149 (MQ=255) gCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCt > 3:63272/1‑149 (MQ=255) gTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGTGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTg < 5:60192/149‑1 (MQ=255) gCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCg > 1:220502/1‑143 (MQ=255) gggTGTTCGTGGGCGGGGGCCTGCCGGGGCCCTACATGGCTCCGCGCTTGGCGCGCAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGc < 3:68826/148‑1 (MQ=255) cggTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGc > 6:43420/1‑149 (MQ=255) ccTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCTACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcggc > 4:192080/1‑149 (MQ=255) tGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcggcg > 6:29697/1‑148 (MQ=255) tGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGAcc > 8:75963/1‑149 (MQ=255) gTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTgcg > 3:250167/1‑148 (MQ=255) gCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTgcgc > 3:280001/1‑147 (MQ=255) cTCAGCGCAATGCGCTGAGCCTCGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGcc > 7:258462/1‑149 (MQ=255) cTCAGCGCAATGCGCTGAGCCTCGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGcc < 8:258462/149‑1 (MQ=255) cTCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGc < 7:147008/142‑1 (MQ=255) cTCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGc > 8:147008/1‑142 (MQ=255) tgaGCGCACTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCCCGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGt < 2:321435/143‑1 (MQ=255) tctgcgcAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCAACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTga > 1:321435/4‑146 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCt > 1:27082/1‑149 (MQ=255) gcgcATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTgtccgc > 3:307185/1‑143 (MQ=255) | TCAACCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCTGA > NC_002947/3852971‑3853260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |