Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,892,921 T→G 7.4% H95Q (CAT→CAG)  rarD → chloramphenicol‑sensitive protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,892,9210TG 7.4% 158.1 / 3.4 54H95Q (CAT→CAG) rarDchloramphenicol‑sensitive protein
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base G (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

CCTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGAG  >  NC_002947/3892773‑3893062
                                                                                                                                                    |                                                                                                                                             
ccTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCAt                                                                                                                                               <  5:226265/149‑1 (MQ=255)
ccTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCAt                                                                                                                                               <  6:668/149‑1 (MQ=255)
 cTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATg                                                                                                                                              >  6:290147/1‑149 (MQ=255)
 cTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATg                                                                                                                                              >  7:231478/1‑149 (MQ=255)
    gTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATgg                                                                                                                                             <  3:60913/147‑1 (MQ=255)
       cATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGgcg                                                                                                                                          >  6:158731/1‑147 (MQ=255)
          gCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGcc                                                                                                                                     >  5:34363/1‑149 (MQ=255)
            ttGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTAGGCATCGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCacgctg                                                                                                                                   >  2:382023/1‑144 (MQ=255)
              gTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTg                                                                                                                                   <  2:396389/147‑1 (MQ=255)
               tctcGAAAGACTGGAAGCGGGTGGGCTAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGaa                                                                                                                                <  8:180666/149‑1 (MQ=255)
               tctcGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGaa                                                                                                                                <  5:406486/149‑1 (MQ=255)
                ctcGAATGTCTGGATGCGGGGGGGCGAACTGATGGGCCGGGTAAAACGCTCGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGaa                                                                                                                                <  7:220904/148‑1 (MQ=255)
                  cGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAACCGCGCGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCGGATGGGGGTGCAGCTGTGGCTGTTTCTCTGGGCGCCCCGGCTTGGGCGCAGCGGGGAAgtg                                                                                                                             >  8:294005/1‑149 (MQ=255)
                     aaGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTc                                                                                                                           >  4:400883/1‑148 (MQ=255)
                         cTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATgg                                                                                                                      <  5:158731/149‑1 (MQ=255)
                         cTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATgg                                                                                                                      <  5:290147/149‑1 (MQ=255)
                            gAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATggg                                                                                                                     <  3:400883/147‑1 (MQ=255)
                                    tGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCctg                                                                                                           <  7:294005/149‑1 (MQ=255)
                                    tGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGGTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCctg                                                                                                           <  6:184006/149‑1 (MQ=255)
                                      ggCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCctgct                                                                                                         <  6:34363/149‑1 (MQ=255)
                                             tGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTg                                                                                                  >  7:199398/1‑149 (MQ=255)
                                               aTGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGc                                                                                                >  3:34506/1‑149 (MQ=255)
                                               aTGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGc                                                                                                <  8:231478/149‑1 (MQ=255)
                                               aTGGGCCGGGTAAAACGCACGCCGGGGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGc                                                                                                <  7:293320/149‑1 (MQ=255)
                                                  ggCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCAt                                                                                             <  4:34506/149‑1 (MQ=255)
                                                        gTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTCCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCt                                                                                       >  4:122217/1‑149 (MQ=255)
                                                               gcacgcCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCggg                                                                                >  6:53476/1‑149 (MQ=255)
                                                                     cGGTGCTGTTGCTCGGCATGGGCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTg                                                                          <  1:47974/149‑1 (MQ=255)
                                                                      ggTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTgg                                                                         <  6:302585/149‑1 (MQ=255)
                                                                             ttGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATgg                                                                  >  6:137823/1‑149 (MQ=255)
                                                                                cTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGc                                                                 >  8:241058/1‑147 (MQ=255)
                                                                                 tCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAg                                                              >  1:44648/1‑149 (MQ=255)
                                                                                 tCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGCCCAGGGTCCTGACCGGGCGGCTGGTATATCGCGAg                                                              >  2:345293/1‑149 (MQ=255)
                                                                                      aTGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCt                                                         >  6:157786/1‑149 (MQ=255)
                                                                                          tCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCa                                                     <  3:149720/149‑1 (MQ=255)
                                                                                          tCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCAAGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCa                                                     >  5:291114/1‑149 (MQ=255)
                                                                                              cACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGAGGGGTTACTTCCTCATGCCACTGGCCATGGTCCTGACCGGGCGGCTCGTATATGGCGAGCGGCTGTCACGcc                                                 >  8:363232/1‑149 (MQ=255)
                                                                                                  tCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCa                                             <  8:199398/149‑1 (MQ=255)
                                                                                                    cTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTAGTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGa                                           >  8:225198/1‑149 (MQ=255)
                                                                                                    cTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCg                                                                                  >  1:37050/1‑110 (MQ=255)
                                                                                                    cTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCg                                                                                  <  2:37050/110‑1 (MQ=255)
                                                                                                                    cAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCt                            <  7:241058/148‑1 (MQ=255)
                                                                                                                     aaCTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGCCCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTgc                          >  2:409184/1‑149 (MQ=255)
                                                                                                                     aaCTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTgc                          >  5:21624/1‑149 (MQ=255)
                                                                                                                                    tctGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTg           >  2:274603/1‑149 (MQ=255)
                                                                                                                                      tGGGCGCCCCTGCATGGGCGCAGCCTGGACGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTgg          <  6:179479/148‑1 (MQ=255)
                                                                                                                                      gctgcgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTgg                               <  7:144626/124‑1 (MQ=255)
                                                                                                                                      gctgcgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTgg                               >  8:144626/4‑127 (MQ=255)
                                                                                                                                       gggCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGc        >  4:34184/1‑149 (MQ=255)
                                                                                                                                       ctgcgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGAc                                          <  2:184042/113‑1 (MQ=255)
                                                                                                                                       ctgcgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGAc                                          >  1:184042/3‑115 (MQ=255)
                                                                                                                                        ggagcCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTgg          <  6:173407/143‑1 (MQ=255)
                                                                                                                                        ggCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTgg          <  5:157786/146‑1 (MQ=255)
                                                                                                                                        ggCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTgg          <  2:44648/146‑1 (MQ=255)
                                                                                                                                            ccccGGCATGGGCGCAGCCGGGACGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCGGTCCCGCCTGCAGACGGGAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGa   <  1:409184/149‑1 (MQ=255)
                                                                                                                                             cccTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGAg  >  5:390236/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CCTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCTCGGCATGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTGTGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGAG  >  NC_002947/3892773‑3893062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑