Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,914,433 G→C 9.5% R118R (CGC→CGG)  PP_3454 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,914,4330GC 9.5% 172.3 / 7.1 61R118R (CGC→CGG) PP_3454transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base C (4/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

ACCACCAGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCC  >  NC_002947/3914293‑3914580
                                                                                                                                            |                                                                                                                                                   
accaccAGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGcagcag                                                                                                                                             >  3:319964/1‑149 (MQ=255)
 ccaccaGCAGGTTGTTCTGACCGCTGGACAGCTCCACGTCCTCGCCGCGCCTACTCACCGGGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGAGCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCagcagc                                                                                                                                            <  7:334057/149‑1 (MQ=255)
 ccaccaGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGCCGcagcagt                                                                                                                                            <  7:160907/149‑2 (MQ=255)
     cagcagGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACTCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTgc                                                                                                                                        <  6:56973/149‑1 (MQ=255)
              gTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGGCGTTGTCCAGGCGCTGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCgg                                                                                                                                <  7:209393/148‑1 (MQ=255)
                    aCCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCcag                                                                                                                           <  4:319964/147‑1 (MQ=255)
                    aCCGCTGGACAGCTCCACCTCATCGCCGCGCCAACTCACCGTGCGCTAGTACAGGTCGATACGCACGCCACCAATCAGGATCAGGTAGTTGTCCAGGCGAGGTTACTTGACACTGCTGCGGGGCAGCAGCGTGCGCACCCGGGCcag                                                                                                                           >  6:336946/1‑147 (MQ=255)
                       gCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAgcagca                                                                                                                      >  5:85265/1‑149 (MQ=255)
                           gACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCgcag                                                                                                                                                <  8:233032/119‑1 (MQ=255)
                           gACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCgcag                                                                                                                                                >  7:233032/1‑119 (MQ=255)
                             cAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTg                                                                                                                <  1:263388/149‑1 (MQ=255)
                                cTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCt                                                                                                             >  1:88032/1‑149 (MQ=255)
                                cTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCt                                                                                                             >  4:47459/1‑149 (MQ=255)
                                  ccACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTc                                                                                                            >  3:233838/1‑148 (MQ=255)
                                       tcctcGCCGCGCAAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGCAACAGGTCGTTGTCCAGGAGCGGTTCGTTGACACTGCTGCGCCGCAGAAGCGAGCGCACCCGGGCCACCAGCACGCGTGGCTCACAggg                                                                                                      >  6:214940/1‑149 (MQ=255)
                                          tcGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGttt                                                                                                   >  1:142248/1‑149 (MQ=255)
                                            gcctcGCCAACTCACCGTGCGCTCGGCCCGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTg                                                                                                  <  5:368094/148‑1 (MQ=255)
                                            gccgcGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGTGttt                                                                                                   <  5:214940/147‑1 (MQ=255)
                                            gccgcGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTg                                                                                                  <  6:85265/148‑1 (MQ=255)
                                            gccgcGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTg                                                                                                  <  4:7509/148‑1 (MQ=255)
                                              cgcgCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTc                                                                                               <  3:369193/149‑1 (MQ=255)
                                               gcgcCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCa                                                                                              <  2:142248/149‑1 (MQ=255)
                                                       tCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAgtcg                                                                                      >  1:207565/1‑149 (MQ=255)
                                                        cACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACCCTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAgtcgt                                                                                     >  6:120844/1‑149 (MQ=255)
                                                               cgcTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGccc                                                                               <  4:233838/148‑1 (MQ=255)
                                                                gcTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCca                                                                                                                            <  2:226884/102‑1 (MQ=255)
                                                                gcTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGcccc                                                                              >  3:237525/1‑148 (MQ=255)
                                                                  tCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCAt                                                                           >  7:175272/1‑149 (MQ=255)
                                                                     gCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTAGTCACGTAGTCGTCGGCCCCCATTTc                                                                        <  2:88032/149‑1 (MQ=255)
                                                                       cAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCCCAGGGTTTGGTCACGTAGTAGTCTGCCCCCATTTc                                                                        >  6:309839/1‑147 (MQ=255)
                                                                             gATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGcc                                                                  >  1:189959/1‑147 (MQ=255)
                                                                                    aGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTg                                                         >  7:104331/1‑149 (MQ=255)
                                                                                    aGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTg                                                         >  3:353089/1‑149 (MQ=255)
                                                                                      gCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGt                                                       <  4:353089/149‑1 (MQ=255)
                                                                                             atcaggatcaggTCGTTGTCCAGGCGCGGTACGTTGACACTCCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACCCGTGGCTCACAGGCTTTCGTCACGTAGTCGTCGGCCCCCATTACCAGGCCTAGCACCTGGTCATGGc                                                 >  8:36891/1‑148 (MQ=255)
                                                                                              tcaggatcaggTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTa                                               >  8:168737/1‑149 (MQ=255)
                                                                                                 ggatcaggTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTAtcg                                            >  2:156975/1‑149 (MQ=255)
                                                                                                 ggatcaggTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGACAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGGTAtcg                                            >  2:116259/1‑149 (MQ=255)
                                                                                                 ggaacaggTCGTTGTCCAGGCGCGGTTCGTTTACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGATTGGTCACGTAGTCGTCGCCCACCATTTCCAGGCCTAGAACCTGGACATGGCTAtc                                             >  6:237543/1‑148 (MQ=255)
                                                                                                      atgTCGTTGTCCAGGCTCGGTTCGTTGACTCTGCTGCGGCGCAGCAGCGTGCGCAGCCGGGCCAGCAGCACGCGTGGCTCACTTGGTTTGGTCACGTAGTCGTCGGCCCCCATTTGCAGGCCTAGCACCTGGTCATGGCTATCGTcgcg                                       <  7:36891/147‑1 (MQ=255)
                                                                                                        gTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCggg                                     >  3:299379/1‑149 (MQ=255)
                                                                                                        gTCGGTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGGGGCGCACAGGGGGTGGTCACGTAGGCGGCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCggg                                     <  5:309839/149‑1 (MQ=255)
                                                                                                            ttGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGt                                 >  7:74774/1‑149 (MQ=255)
                                                                                                              gTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGt                                 >  5:123278/1‑147 (MQ=255)
                                                                                                              gTCCAGGCGCGGTTCGTTGACACTGCTGCGGAGCAGCAGCGTGCGCACCCGGGCCACCAGCACGCGTGGCTCACAGGGATTGGTCACGTACTCGTCGGCACCCATTTCCAGCCCAAGCACCTGGTCATGGCTATCTTCGCGGGCCGTc                                <  5:336946/148‑1 (MQ=255)
                                                                                                                 cAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGGGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCa                            <  3:298147/149‑1 (MQ=255)
                                                                                                                     cgcgGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAg                        <  3:197566/149‑1 (MQ=255)
                                                                                                                      gcgGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAgg                       <  7:168737/149‑1 (MQ=255)
                                                                                                                               gcacgctgctgCGCCGCAGCAGTGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              >  5:67719/6‑149 (MQ=255)
                                                                                                                               gcacgctgctgCGCCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              >  8:94408/6‑149 (MQ=255)
                                                                                                                                gACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              <  6:123278/148‑1 (MQ=255)
                                                                                                                                cacgctgctgCGCCGCAGCAGTGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              <  4:26966/144‑1 (MQ=255)
                                                                                                                                cacgctgctgCGCCGCAGCAGTGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              >  3:26966/5‑148 (MQ=255)
                                                                                                                                 acacTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCgg                                      >  7:110536/1‑123 (MQ=255)
                                                                                                                                 acacTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCgg                                      <  8:110536/123‑1 (MQ=255)
                                                                                                                                 acacTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAggg             <  1:29290/148‑1 (MQ=255)
                                                                                                                                   acTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCt          >  3:15334/1‑149 (MQ=255)
                                                                                                                                       ctgcGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              <  8:283346/141‑1 (MQ=255)
                                                                                                                                       ctgcGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg              >  7:283346/1‑141 (MQ=255)
                                                                                                                                       ctgcGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGc        <  1:156975/147‑1 (MQ=255)
                                                                                                                                        tgcGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCGCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGAc     >  5:262300/1‑149 (MQ=255)
                                                                                                                                          cggcgCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGc        <  3:82839/144‑1 (MQ=255)
                                                                                                                                          cggcgCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTc   <  8:74774/149‑1 (MQ=255)
                                                                                                                                          cggcgCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGCGTCTGc        >  4:82839/1‑144 (MQ=255)
                                                                                                                                           ggcgCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTcc  <  8:175272/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
ACCACCAGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCAGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAGCGTGCGCACCCGGGCCAGCAGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCC  >  NC_002947/3914293‑3914580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑