Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,982,841	A→C	7.0%	*120C (TGA→TGC)	PP_3510 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,982,841	0	A	C	7.0%	241.1 / 8.0	83	*120C (TGA→TGC)	PP_3510	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/37);  new base C (2/4);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

GGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGGTGTCAT  >  NC_002947/3982694‑3982988                                                                                                                                                     |                                                                                                                                                     ggCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGAc                                                                                                                                                     <  3:6293/149‑1 (MQ=255)      gTATGCTGGCACTGACCTGCAAGGCATTGGAACACGCCGTAATACAGCTGGACAAACCCGCAT‑GGCATGTACGCATTGACAGCGAAGAGGCCTGTGCACTGCTTCATGGCGTGGACTCGGGAAACCGCCTGCAGCGGCATTGACCCTGc                                                                                                                                                >  4:271871/1‑149 (MQ=255)         tGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCgg                                                                                                                                              <  5:326457/148‑1 (MQ=255)         tGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGt                                                                                                                                             <  6:284912/149‑1 (MQ=255)           cTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcaggc                                                                                                                                            <  4:197249/148‑5 (MQ=255)           cTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcaggc                                                                                                                                            <  4:190939/148‑5 (MQ=255)           cTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcaggc                                                                                                                                            <  3:181937/148‑5 (MQ=255)           cTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                           <  7:334505/149‑1 (MQ=255)           cTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                           <  3:135556/149‑1 (MQ=255)           cTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGttt                                                                                                                                           >  2:110417/1‑149 (MQ=255)            tGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACCGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGCAAACGCCTGCAGGGGCATTGACCCTGCGGgtt                                                                                                                                           >  7:269936/1‑148 (MQ=255)              gCACTGACCTGCCAGTCATTGGACCAGGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGATGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTg                                                                                                                                          <  3:271871/147‑1 (MQ=255)              gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGa                                                                                                                                         <  8:208785/148‑1 (MQ=255)               cACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGAc                                                                                                                                        >  1:105642/1‑149 (MQ=255)               cACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGAc                                                                                                                                        >  5:65594/1‑148 (MQ=255)               cACTGACATGCCAGGCATTGGAGATCGAGGGAATTCATCTGGACCATCCCTCTT‑GGCATGTACGCTATGACAGCGTAGAGGCGTGTGCTCGGGTGCATGGCGGGGACTGGGGATAACGGCTGCAGGGGTATTGACCCTGCGGttttag                                                                                                                                        <  4:305761/148‑4 (MQ=255)                aCTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                                      >  3:173617/1‑149 (MQ=255)                aCTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                                      <  3:318687/149‑1 (MQ=255)                  tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACg                                                                                                                                       <  6:278213/146‑1 (MQ=255)                  tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACg                                                                                                                                       >  5:278213/1‑146 (MQ=255)                    aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATa                                                                                                                                    <  1:39299/147‑1 (MQ=255)                      cTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCCGCAGGGTCAATGCCCCTGcag                                                                                                                                              <  6:102387/135‑3 (MQ=255)                      cTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCCGCAGGGTCAATGCCCCTGcag                                                                                                                                              >  5:102387/1‑133 (MQ=255)                       tGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATc                                                                                                                               >  1:249394/1‑149 (MQ=255)                               aTTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTg                                                                                                                                                 >  7:152970/1‑123 (MQ=255)                               aTTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTg                                                                                                                                                 <  8:152970/123‑1 (MQ=255)                                ttGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCAc                                                                                                                     >  4:83381/1‑149 (MQ=255)                                  ggACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCAcc                                                                                                                    <  6:65594/148‑1 (MQ=255)                                           cGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCaaatcaaatc                                                                                                          >  4:297867/1‑149 (MQ=255)                                           cGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTAt                                                                                                                <  8:362657/143‑1 (MQ=255)                                           cGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTAt                                                                                                                >  7:362657/1‑143 (MQ=255)                                           cGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTc                                                                                                          <  2:105642/149‑1 (MQ=255)                                             gAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGt                                                                                                        >  4:142000/1‑149 (MQ=255)                                                 aCAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACt                                                                                                    >  2:394855/1‑149 (MQ=255)                                                  cAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTg                                                                                                   >  6:35980/1‑149 (MQ=255)                                                  cAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTg                                                                                                   >  6:41882/1‑149 (MQ=255)                                                  cAGATGGACCATACCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGTAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTg                                                                                                   >  1:326411/1‑149 (MQ=255)                                                   aGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCa                                                                                                                      >  1:34199/1‑129 (MQ=255)                                                   aGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCa                                                                                                                      <  2:34199/129‑1 (MQ=255)                                                      tGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCg                                                                                               >  3:401749/1‑149 (MQ=255)                                                        gACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGt                                                                                              <  4:401749/148‑1 (MQ=255)                                                          ccATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTcc                                                                                           <  7:179929/149‑1 (MQ=255)                                                           cATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGTCGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTccc                                                                                          >  7:253637/1‑149 (MQ=255)                                                              cccGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGAt                                                                                       >  6:123668/1‑149 (MQ=255)                                                                 gCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCg                                                                                    <  2:61189/149‑1 (MQ=255)                                                                          tGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGTGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGAt                                                                            <  5:41882/149‑1 (MQ=255)                                                                          tGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGAt                                                                            <  5:35980/149‑1 (MQ=255)                                                                                 tATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTAtt                                                                       >  1:71577/1‑147 (MQ=255)                                                                                   ttaCAGCGAAGAGTCCTGTGCACGGGTGCATGGCGGGTACTTGGGAAAACGCCTGCAGGGGCATTGTCCCTGCGGTTTGACGATAGTATCGCATTCGCACCCTATCAATTCGTGACTGGGCGTTCCCGATCCGCCAAGGATGTATTAAg                                                                    <  1:110417/147‑1 (MQ=255)                                                                                     aCAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGt                                                                  <  2:71577/148‑1 (MQ=255)                                                                                               ggCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTgc                                                        <  1:394855/148‑1 (MQ=255)                                                                                                gCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTgcgc                                                      <  2:52387/149‑1 (MQ=255)                                                                                                gCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTgcg                                                       >  2:381811/1‑148 (MQ=255)                                                                                                   tgtgCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCata                                                   >  8:63754/1‑149 (MQ=255)                                                                                                       cACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATaa                                                >  3:282356/1‑148 (MQ=255)                                                                                                       cACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATaa                                                <  1:381811/148‑1 (MQ=255)                                                                                                       cACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAc                                               <  3:297867/149‑1 (MQ=255)                                                                                                          gggTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAcag                                             >  2:405525/1‑148 (MQ=255)                                                                                                           ggTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAcagca                                           <  3:83381/149‑1 (MQ=255)                                                                                                              gCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTc                                                                                                          <  3:272167/83‑1 (MQ=255)                                                                                                              gCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTc                                                                                                          >  4:272167/1‑83 (MQ=255)                                                                                                                 tGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAgg                                                                   <  2:277878/119‑1 (MQ=255)                                                                                                                 tGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAgg                                                                   >  1:277878/1‑119 (MQ=255)                                                                                                                          cTGGGTAAAACTCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGAGCATATAACAGCAGCCCGAGCGATCggg                            <  2:326411/149‑1 (MQ=255)                                                                                                                            ggggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGc                           >  7:335381/1‑148 (MQ=255)                                                                                                                             gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCAc                         >  1:137781/1‑149 (MQ=255)                                                                                                                             gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCAc                         >  3:298879/1‑149 (MQ=255)                                                                                                                             gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGAGGCATGGTGCGCATATAACAGCAGCCCGAg                                    <  1:34303/138‑1 (MQ=255)                                                                                                                             gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGAGGCATGGTGCGCATATAACAGCAGCCCGAg                                    >  2:34303/1‑138 (MQ=255)                                                                                                                             gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTACCGATCAGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCAc                         >  3:250950/1‑149 (MQ=255)                                                                                                                               gAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACtt                       >  7:164724/1‑149 (MQ=255)                                                                                                                                 aaaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTACCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCatat                                                  >  5:318781/1‑120 (MQ=255)                                                                                                                                     gCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCATCCCGAGCGATCGGGCACTTGCAAGa                 <  1:405525/149‑1 (MQ=255)                                                                                                                                     gCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGa                 >  1:317847/1‑149 (MQ=255)                                                                                                                                     aaccgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGa                 >  3:113298/5‑149 (MQ=255)                                                                                                                                       cTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGAc                >  6:402723/1‑148 (MQ=255)                                                                                                                                           aGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa                   <  3:210046/141‑1 (MQ=255)                                                                                                                                           aGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa                   >  4:210046/1‑141 (MQ=255)                                                                                                                                            ggggCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAg          >  7:106274/1‑149 (MQ=255)                                                                                                                                             gggCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAgg         <  2:116379/149‑1 (MQ=255)                                                                                                                                             gggCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAgg         <  2:104848/149‑1 (MQ=255)                                                                                                                                               gCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGgt       <  3:142000/149‑1 (MQ=255)                                                                                                                                               gCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGgt       <  5:402723/149‑1 (MQ=255)                                                                                                                                                     aCCCTGCGGTTTGTCGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGGTGTCAt  <  8:253637/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTT‑GGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGC‑TTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAGGGTGTCAT  >  NC_002947/3982694‑3982988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑