| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.0% | 103.1 / ‑2.9 | 33 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (17/15); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
GGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932492‑1932776 | ggcggcGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑cc > 1:336766/1‑149 (MQ=255) gcggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑ccc < 6:365056/149‑2 (MQ=255) gcggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑cc < 4:347365/148‑1 (MQ=255) tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAg < 4:303285/149‑1 (MQ=255) tGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGcc > 1:195201/1‑149 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCa < 3:40819/144‑1 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGGATATGCGTCCCCAGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGccc > 3:221583/1‑148 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCaaa > 5:287507/1‑149 (MQ=255) cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGAc > 1:18435/1‑148 (MQ=255) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTa > 7:175001/1‑149 (MQ=255) atgttctcAAGAAAATCGATGTAACCGCGGGGCCCGCTTTGCGGCCGTTCGCGGGAACGCCCGCTCCTACAACGTATTTGGGTCCCCTGTTGTTGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGAt < 8:281478/145‑1 (MQ=255) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATc > 8:344385/1‑149 (MQ=255) ggTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 6:287507/149‑1 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑ccc > 7:96016/1‑102 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑ccc < 8:96016/103‑2 (MQ=255) aTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCGCTGATCCgcgta < 7:344385/149‑5 (MQ=37) aaGCAGAAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGCCCCCTGTAGGAGGGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 8:280740/148‑1 (MQ=37) gCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCgcgtagtc < 2:115076/149‑8 (MQ=255) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCCAAGGCAGCCCCAAATTGTCGACTAcgc < 1:10369/126‑1 (MQ=255) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTAcgc > 2:10369/1‑125 (MQ=255) gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCAAAAAAGGATATGGGTGCCCAGTAGGAGCGGGCTTTC‑CCGCGAAAGGCTT‑CAAGGCAGCCCCAAATTGTAGACAAAGCCGATAAGCGATACGGCAGGGTGATGTTGAGCTACa > 8:230170/1‑148 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCCGCGATCCGGCAGGGTGATg > 5:71106/1‑136 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGACGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGc > 7:342600/1‑127 (MQ=255) ggCAAGCCCGCTCATACAACGTATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt < 7:230170/149‑1 (MQ=39) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 2:18435/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 4:353772/1‑149 (MQ=255) aGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 7:139703/1‑149 (MQ=255) gATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat > 6:292166/1‑148 (MQ=255) tGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTc > 2:355856/1‑59 (MQ=18) tGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTc < 1:355856/59‑1 (MQ=18) gCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 5:81531/1‑149 (MQ=255) tCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 6:81531/149‑1 (MQ=255) cTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 7:326145/1‑149 (MQ=255) tAGGAGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 2:195201/149‑1 (MQ=255) aGCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcac < 8:175001/148‑1 (MQ=255) gCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCCGCGATCCGGCAGGGTGAGGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATAATCGTTTCCGATGTTcaga > 1:133767/1‑146 (MQ=255) gCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt < 4:207788/109‑1 (MQ=255) gCGGGC‑TTGCCCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 3:207788/1‑109 (MQ=255) | GGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTG‑CAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932492‑1932776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |