Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,845,705	+G	intergenic (‑72/+26)	PP_3394 ← / ← PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,845,703	1	.	G	100.0%	148.8 / NA	44	intergenic (‑70/+28)	PP_3394/PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/22);  total (22/22)

GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACC  >  NC_002947/3845556‑3845838                                                                                                                                                     |                                                                                                                                           ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCTGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcgc                                                                                                                                             <  2:234607/148‑1 (MQ=255)  gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gg                                                                                                                                          <  6:99618/149‑1 (MQ=255)    tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCTCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc                                                                                                                                        <  4:64716/149‑3 (MQ=255)      ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc                                                                                                                                        <  4:190924/147‑3 (MQ=255)                gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGt                                                                                                                              >  5:286328/1‑148 (MQ=255)                gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGt                                                                                                                              >  2:301536/1‑148 (MQ=255)                 aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCa                                                                                                                            <  2:152520/149‑1 (MQ=255)                    aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGc                                                                                                                          <  4:321479/148‑1 (MQ=255)                        gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATc                                                                                                                                    <  5:319824/134‑1 (MQ=255)                        gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATc                                                                                                                                    >  6:319824/1‑134 (MQ=255)                        gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACg                                                                                                                      <  4:54624/148‑1 (MQ=255)                         cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGa                                                                                                                     <  2:158897/148‑1 (MQ=255)                         cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGa                                                                                                                     <  8:176976/148‑1 (MQ=255)                         cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGa                                                                                                                     <  7:23743/148‑1 (MQ=255)                           ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATgg                                                                                                                  <  4:111987/149‑1 (MQ=255)                             ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                <  1:301536/149‑1 (MQ=255)                             ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGAAACCTCGTCACCCACCAAGGa                                                                                                                 >  3:208276/1‑148 (MQ=255)                              tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                >  8:204323/1‑148 (MQ=255)                                tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                <  7:204323/146‑1 (MQ=255)                                  aaaGCCATCTCGCCGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTGGCTGACGATGGATCa                                                                                                              <  6:261165/145‑1 (MQ=255)                                    aGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCcg                                                                                                         >  2:219138/1‑149 (MQ=255)                                        aTCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGc‑‑‑c                                                                                                  >  7:168034/1‑149 (MQ=255)                                             gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGcc                                                                                             <  1:219138/149‑1 (MQ=255)                                                 gCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGc                                                                                         >  8:318714/1‑149 (MQ=255)                                                  ccGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCAAGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGcc                                                                                        >  7:47928/1‑149 (MQ=255)                                                       ttCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGc                                                                                   >  6:308067/1‑149 (MQ=255)                                                              attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCa                                                                                                                                  <  2:4026/98‑1 (MQ=255)                                                              attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCa                                                                                                                                  >  1:4026/1‑98 (MQ=255)                                                              attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACgcag                                                                             >  2:306281/1‑148 (MQ=255)                                                                    attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAgg                                                                      >  3:252028/1‑149 (MQ=255)                                                                        ttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCGGCCAGGCCGGTGCACGCAGCAACAggtggt                                                                  >  4:120891/1‑149 (MQ=255)                                                                        ttggCATCGCGGTCTCTCTGGCTGACGCGTTGCGCCAGACTAAGCGCTGGATGACGGGCAGAAAAGCCGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGc‑‑‑c                                                                                                  <  7:347480/117‑1 (MQ=255)                                                                          gtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc                                                                 >  5:264113/1‑148 (MQ=255)                                                                             aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCg                                                                                       <  5:175178/123‑1 (MQ=255)                                                                             aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCg                                                                                       >  6:175178/1‑123 (MQ=255)                                                                                   ggctccctGTCTGAAGCGTGGCGCCAGACTAAGAGCGGGATGCCGGGCATAAAAGACGTGTTGCGCGGGCTGAGCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgcgc                                                       <  6:286328/147‑1 (MQ=255)                                                                                        tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                                  >  5:136625/1‑149 (MQ=255)                                                                                           gtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCAGCCAGGCCCGTGCACGCAGCAACAGGTGGTCAACCAGGCgccgcgccga                                               >  6:325420/1‑149 (MQ=255)                                                                                                   cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga                                       <  8:168034/149‑1 (MQ=255)                                                                                                     ttGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCAGATCCATCGTCAGCCACCATGGATCAGCCCCCGC‑‑‑CCTTCCAGGCCGGTGCACCCAGCAACAGGGGGTCAAGCAGGCGCCCAGCCGACCGCGAGAGc                                     >  4:53137/1‑149 (MQ=255)                                                                                                                            gACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGa               <  6:136625/148‑1 (MQ=255)                                                                                                                              cGGGCACAAAAGCCGTGTGTCGCGGGCTCACCCAGCGTCCCCCACGAGGTATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGa            <  3:120891/149‑1 (MQ=255)                                                                                                                                  cAGAAACGCGGTGTTGCGCGGGCTGACCCATCGTCCCCCACGATGCATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa        <  7:318714/149‑1 (MQ=255)                                                                                                                                  aaGAAAAGACGTGTTTGGCGGGCTGACCCATCGGCCGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa        <  6:264113/148‑1 (MQ=255)                                                                                                                                     aaaaGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt      >  6:88949/1‑148 (MQ=255)                                                                                                                                        aGACGTGTTTCGCGGGCTTATCCATCGTCAGCCACGATGGATCAGCCCGGGCTCACTTGCCCCCCCCCTCCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc     <  1:306281/149‑1 (MQ=255)                                                                                                                                         gACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAcc  >  2:274267/1‑148 (MQ=255)                                                                                                                                          aCGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAcc  >  3:352627/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                           GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGC‑‑‑CCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACC  >  NC_002947/3845556‑3845838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑