Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,751	+G	intergenic (+113/+27)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,750	1	.	G	100.0%	231.0 / NA	67	intergenic (+112/+28)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (32/35);  total (32/35)

CGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG  >  NC_002947/5674602‑5674889                                                                                                                                                       |                                                                                                                                            cgGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTCCAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAgc                                                                                                                                              <  5:289255/149‑1 (MQ=255)  ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAgc‑g                                                                                                                                            >  2:101543/1‑149 (MQ=255)                gctgctGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGtttt                                                                                                                               <  3:295283/149‑1 (MQ=255)                    ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            >  8:208604/1‑148 (MQ=255)                       aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        <  8:163140/149‑1 (MQ=255)                         ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        >  8:88707/1‑147 (MQ=255)                         ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        >  2:316514/1‑147 (MQ=255)                           gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                 <  7:284378/136‑1 (MQ=255)                           gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                 >  8:284378/1‑136 (MQ=255)                                ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            <  7:203197/136‑1 (MQ=255)                                ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            >  8:203197/1‑136 (MQ=255)                                ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTa                                                                                                               <  6:135383/149‑1 (MQ=255)                                     aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                           <  7:312956/148‑1 (MQ=255)                                     aTAGAAGTACCGACGGTGCTGCTTCGCAGCCCTATCACGACACAAGTACGATCCTGCAGAGAATTTCTTGATCTCTACATGAGCGGCCTTGTGTCGT‑TATAGGGCCGCAAAGCGGCCACCTCGGTTTTCAGCTGTCAGAAATTACTTc                                                                                                           <  5:10876/148‑1 (MQ=255)                                         aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                      >  3:298994/1‑149 (MQ=255)                                          aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTAGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                      <  1:101543/149‑1 (MQ=37)                                           gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca                                                                                                    <  7:208604/149‑1 (MQ=255)                                              ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCacac                                                                                                 <  6:364553/149‑1 (MQ=255)                                                  gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             <  5:97301/149‑1 (MQ=255)                                                      gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAgc‑gg                                                                                                                                           <  2:77305/98‑2 (MQ=255)                                                      gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAgc‑gg                                                                                                                                           >  5:162770/1‑97 (MQ=255)                                                      gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAgc‑gg                                                                                                                                           >  1:77305/1‑97 (MQ=255)                                                      gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAgc‑gg                                                                                                                                           <  6:162770/98‑2 (MQ=255)                                                            tCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGata                                                                                   >  6:151745/1‑149 (MQ=255)                                                             cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                  >  2:16996/1‑149 (MQ=255)                                                              gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                        >  2:291994/1‑126 (MQ=255)                                                              gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                        <  1:291994/126‑1 (MQ=255)                                                               cAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  5:217946/1‑148 (MQ=255)                                                                aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  1:43400/1‑147 (MQ=255)                                                                aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  1:39124/1‑147 (MQ=255)                                                                 gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                              <  7:88707/149‑1 (MQ=255)                                                                 gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                              <  3:290450/149‑1 (MQ=255)                                                                 gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                              <  1:316514/149‑1 (MQ=255)                                                                  cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACTGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                             <  5:35646/149‑1 (MQ=255)                                                                   ccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                            >  2:177554/1‑149 (MQ=255)                                                                     tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                      >  6:239825/1‑137 (MQ=255)                                                                     tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                      <  5:239825/137‑1 (MQ=255)                                                                          cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             >  4:347258/1‑125 (MQ=255)                                                                          cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             <  3:347258/125‑1 (MQ=255)                                                                                aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGc                                                                                                                       <  8:9991/93‑1 (MQ=255)                                                                                aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGc                                                                                                                       >  7:9991/1‑93 (MQ=255)                                                                                aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                >  5:232370/1‑148 (MQ=255)                                                                                    ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                           <  7:256270/149‑1 (MQ=255)                                                                                     cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                          >  3:335206/1‑149 (MQ=255)                                                                                      gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcc                                                                                <  5:350749/126‑1 (MQ=255)                                                                                      gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcc                                                                                >  6:350749/1‑126 (MQ=255)                                                                                      gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGc                                                         <  4:298994/149‑1 (MQ=255)                                                                                         ccTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAcc                                                      <  6:217946/149‑1 (MQ=255)                                                                                           tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                     <  3:308883/116‑1 (MQ=255)                                                                                           tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                     >  4:308883/1‑116 (MQ=255)                                                                                             cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCATGTCCAGGGTCTGCGAAGTAGCACCCgg                                                   <  1:16996/148‑1 (MQ=255)                                                                                             cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                  >  7:138462/1‑149 (MQ=255)                                                                                                     ttCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAATTCTGCTTCACAACTTCGACTATGATATCAAGGTCCAGGGTCAGCGAAGTAGCACCCGGGCCTTTGAt                                          >  8:149768/1‑149 (MQ=38)                                                                                                                tACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                 <  2:39124/147‑1 (MQ=255)                                                                                                                  cAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                                <  2:365835/146‑1 (MQ=255)                                                                                                                     gAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                          >  2:133743/1‑149 (MQ=255)                                                                                                                     gAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTATCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                          >  8:205692/1‑149 (MQ=255)                                                                                                                     gAGCGGCCTTGTGACGA‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCATAAAATACTTCTGATTCACACCTTCGACACTGATAACCAGGTCCAGGGTCTGCGAAGTAGCACCCGGCCCTTTGAAGCCGAAGACgttcagg                          >  6:2187/1‑149 (MQ=255)                                                                                                                               gtgtCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                 <  1:177554/148‑1 (MQ=255)                                                                                                                                tgtCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt               <  1:138562/149‑1 (MQ=255)                                                                                                                                  tCGT‑TATAGGGCCGAAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTTCTTCACACCTTCGACTCTGATATCCATGTCCAGGTTCTGCGATGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGTTTCAGCgtggtgg             <  3:109293/149‑1 (MQ=255)                                                                                                                                  tCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg             >  8:301525/1‑149 (MQ=255)                                                                                                                                  tCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg              <  6:232370/148‑1 (MQ=255)                                                                                                                                     t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGTAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg          <  2:43400/149‑1 (MQ=255)                                                                                                                                     t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                         >  6:214363/1‑134 (MQ=255)                                                                                                                                     t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                         <  5:214363/134‑1 (MQ=255)                                                                                                                                     t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg          >  2:30778/1‑149 (MQ=255)                                                                                                                                         tAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt       <  5:160680/149‑1 (MQ=255)                                                                                                                                            ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                           <  7:227325/126‑1 (MQ=255)                                                                                                                                            ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                           >  8:227325/1‑126 (MQ=255)                                                                                                                                            ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg     >  1:78656/1‑148 (MQ=255)                                                                                                                                             gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg     >  8:97966/1‑147 (MQ=255)                                                                                                                                              ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg  >  7:324860/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                            CGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG  >  NC_002947/5674602‑5674889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑