| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 90.0% | 150.9 / 10.2 | 50 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (5/0); new base C (16/29); total (21/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.60e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑C‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126506‑1126788 | ccgatgccgatCTTGACCCGGGCGAATACGCGCAGTGACATGTGGACCTCCTGCCTGGTTGTTGGGTGT‑ACTTTGAGCCCTGTTGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATGCGC‑GGG‑T‑AAACC‑CGCTCCt < 8:312354/149‑1 (MQ=37) tGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCCCCAAAGCGGAGGTATAGCACCCAACGCAATCGC‑GGG‑TAAAA‑C‑CGCGACGACACAGAGCC‑CGc > 2:82334/1‑149 (MQ=255) tGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTAACCCCCAATGCGGAGGTATACGCACCATCGCATTAGC‑GGG‑TAAAA‑A‑CGCTACAACACAGAGGC‑CGc > 2:87563/1‑149 (MQ=255) aaCAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCa < 1:249124/149‑1 (MQ=255) cGAACACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGGGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGc < 7:132750/149‑1 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCggg < 1:275695/149‑1 (MQ=255) gTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCgggg > 5:363252/1‑149 (MQ=255) cATGTGGCCCCCAGGCCCGGTTGTTGGGGGT‑CCTTTGCGCCCTGTCGGCGCGGGTTTCCCGGCTAATGCGGAGGTAACCCCACCATCGCATCCGC‑GGT‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAggg < 8:190771/149‑1 (MQ=255) cATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAggg < 5:314992/149‑1 (MQ=255) aTGTGGACCCCCTGCCCGGTTGTTGGGTGG‑ACATTGCGCCCGGTAGGCGCGGGTTTACCCGCTAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGc < 8:218798/149‑1 (MQ=38) gtgGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAATGCGGAGGTATAGCCCACATAGCATTAGCGGGG‑T‑AAA‑C‑CGCTGCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTa > 5:286697/1‑149 (MQ=255) ctcctGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTCGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCGAGGTGGCGGGGAGGGCTATCAGGc < 1:87563/149‑1 (MQ=255) ctcctGCCAGGTTGTTGGGGGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc < 1:82334/149‑1 (MQ=255) cctGCCAGGTGGTGGGGTGG‑ACCTTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATACCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGt < 5:210975/149‑1 (MQ=38) tgcctgtttttGGGGGGAACATTCAGCCCTTTCGGCGCGGTTTTACCCGCCAATGCGTAGTTATACCCACAACCGAATTCGG‑GTT‑A‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc < 2:166139/144‑1 (MQ=255) aGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGGGGATGTATTTCCACCATCGCATTAGC‑GGGTT‑AAA‑C‑CGCACCTACACAGAGAC‑CGCCACCCAACCCAGGTGGCGGGGAGGGCTATAACGCGtacaga > 5:108652/1‑143 (MQ=37) tgttgGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATTCGGAGGTATAGCCACCATCGCATTAGC‑GGG‑T‑TAA‑CACGCTCCTAAACAGAGCC‑CGCCACCCAAGCCAGGGGGCGGGGAGGGCTATCAGGCGTCCTGATAg > 7:252097/1‑147 (MQ=255) gCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGAATAGCCCCCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACAGAGAGCC‑CGCCACCCAAGCCTGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAg < 6:89512/148‑1 (MQ=38) cccTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 6:363252/149‑1 (MQ=255) gggTTTACCCGCGAATGGGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGtt < 5:1221/149‑1 (MQ=255) gggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCa < 7:22118/145‑1 (MQ=255) gggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCa > 8:22118/1‑145 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc < 3:26397/148‑1 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg > 2:206534/1‑149 (MQ=255) cgcgAATGCGGAGGTATAGCCGCCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 2:373042/1‑149 (MQ=255) cgcgAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc < 6:286697/149‑1 (MQ=255) gTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc > 3:316434/1‑148 (MQ=255) ataGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa > 1:183352/1‑90 (MQ=255) ataGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 2:183352/90‑1 (MQ=255) ataGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 5:363924/91‑1 (MQ=255) ataGCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt > 6:363924/1‑91 (MQ=255) gccgccATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 1:373042/148‑1 (MQ=255) gCCACCATCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg < 6:108652/149‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 1:340610/1‑149 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 5:326079/1‑149 (MQ=255) caccaTCGCATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 3:21029/1‑149 (MQ=255) cATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAgg < 5:289172/99‑1 (MQ=255) cATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAgg > 6:289172/1‑99 (MQ=255) cATTCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc < 3:64311/149‑1 (MQ=255) ttCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCCTCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcg > 1:39601/1‑149 (MQ=255) ttCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATTAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcgg > 4:130664/1‑149 (MQ=255) tCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt < 6:326190/127‑1 (MQ=255) tCGC‑GGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 5:326190/1‑127 (MQ=255) cgc‑gGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATTAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 3:130664/148‑1 (MQ=255) cgc‑gGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 4:21029/148‑1 (MQ=255) gc‑gGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg > 1:316254/1‑110 (MQ=255) gc‑gGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg < 2:316254/110‑1 (MQ=255) gc‑gGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc > 3:206709/1‑128 (MQ=255) gc‑gGG‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc < 4:206709/128‑1 (MQ=255) g‑T‑AAACC‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc < 4:303505/149‑1 (MQ=255) | CCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGG‑ACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑C‑CGCTCCTACACAGAGCC‑CGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126506‑1126788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |