Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,231	C→G	intergenic (+468/+285)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,231	0	C	G	100.0%	114.8 / NA	34	intergenic (+468/+285)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/18);  total (16/18)

CGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCT  >  NC_002947/4741124‑4741361                                                                                                            |                                                                                                                                     cGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCg                                                                                              >  8:156728/1‑148 (MQ=255)     tcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACGGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCggacac                                                                                         >  1:77437/1‑144 (MQ=255)       ctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCggacac                                                                                         <  7:156728/147‑6 (MQ=255)             ggTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                 >  2:87900/1‑148 (MQ=255)              gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                <  7:141206/149‑3 (MQ=255)                  tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGc                                                                            <  1:155747/149‑1 (MQ=255)                        gcagcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctcg                                                                      >  3:28701/1‑146 (MQ=255)                                      cgcgGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAgcgg                                                       <  7:88475/149‑1 (MQ=255)                                      cgcgGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAgcgg                                                       >  6:29747/1‑149 (MQ=255)                                               aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGAt                                              >  3:219391/1‑149 (MQ=255)                                                ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTAAATCCAGGCACAGGCCCCGGACACTTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATc                                             >  7:55794/1‑149 (MQ=255)                                                   gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACg                                          <  8:206768/149‑1 (MQ=255)                                                   gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACg                                          <  5:29747/149‑1 (MQ=255)                                                    ccATGTCGTATTGAACTGGGTATTGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTTAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACgg                                         <  7:344000/149‑1 (MQ=255)                                                       tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTca                                      <  7:89813/149‑1 (MQ=255)                                                        ggCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTca                                      >  3:141786/1‑148 (MQ=255)                                                           gTATTGAACTAGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACAcc                                  >  3:153862/1‑149 (MQ=255)                                                                 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACg                                          >  3:95304/1‑135 (MQ=255)                                                                 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACg                                          <  4:95304/135‑1 (MQ=255)                                                                  aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                           <  2:77437/149‑1 (MQ=255)                                                                  aCTAGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                            <  4:153862/148‑1 (MQ=255)                                                                       gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                      >  6:89677/1‑149 (MQ=255)                                                                        tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg                     <  4:141786/149‑1 (MQ=255)                                                                             gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                >  1:346199/1‑149 (MQ=255)                                                                                cAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGCGCCAGCGGCGCCTTGATCACGGTCACACCACGATAGGCCTTGTACTTg               <  1:87900/148‑1 (MQ=255)                                                                                      tGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACTTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc       >  6:377828/1‑149 (MQ=255)                                                                                      tGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGCACTAG‑GCCAGCGGCGCCTTGATCACGGTCAAACCGCGATAGGCCTTGTACTTGGACCCgga       >  6:312209/1‑148 (MQ=255)                                                                                        gTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCgg        <  2:346199/146‑1 (MQ=255)                                                                                        gTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc       <  2:305690/147‑1 (MQ=255)                                                                                        gTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa     >  5:124011/1‑149 (MQ=255)                                                                                           aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt  <  4:28701/149‑1 (MQ=255)                                                                                           aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt  <  4:219391/149‑1 (MQ=255)                                                                                             cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                         >  4:118671/1‑124 (MQ=255)                                                                                             cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                         <  3:118671/124‑1 (MQ=255)                                                                                                            |                                                                                                                                     CGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTG‑GCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCT  >  NC_002947/4741124‑4741361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑