Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	229.7 / NA	67	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (32/35);  total (32/35)

GCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG  >  NC_002947/5674617‑5674889                                                                                                                                           |                                                                                                                                         gctgctGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGtttt                                                                                                                               <  3:295283/149‑1 (MQ=255)     ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            >  8:208604/1‑148 (MQ=255)        aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        <  8:163140/149‑1 (MQ=255)          ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        >  8:88707/1‑147 (MQ=255)          ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        >  2:316514/1‑147 (MQ=255)            gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                 >  8:284378/1‑136 (MQ=255)            gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                 <  7:284378/136‑1 (MQ=255)                 ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            <  7:203197/136‑1 (MQ=255)                 ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            >  8:203197/1‑136 (MQ=255)                 ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTa                                                                                                               <  6:135383/149‑1 (MQ=255)                      aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                           <  7:312956/148‑1 (MQ=255)                      aTAGAAGTACCGACGGTGCTGCTTCGCAGCCCTATCACGACACAAGTACGATCCTGCAGAGAATTTCTTGATCTCTACATGAGCGGCCTTGTGTCGT‑TATAGGGCCGCAAAGCGGCCACCTCGGTTTTCAGCTGTCAGAAATTACTTc                                                                                                           <  5:10876/148‑1 (MQ=255)                          aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                      >  3:298994/1‑149 (MQ=255)                           aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTAGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                      <  1:101543/149‑1 (MQ=37)                            gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca                                                                                                    <  7:208604/149‑1 (MQ=255)                               ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCacac                                                                                                 <  6:364553/149‑1 (MQ=255)                                   gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             <  5:97301/149‑1 (MQ=255)                                             tCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGata                                                                                   >  6:151745/1‑149 (MQ=255)                                              cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                  >  2:16996/1‑149 (MQ=255)                                               gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                        <  1:291994/126‑1 (MQ=255)                                               gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                        >  2:291994/1‑126 (MQ=255)                                                cAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  5:217946/1‑148 (MQ=255)                                                 aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  1:43400/1‑147 (MQ=255)                                                 aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  1:39124/1‑147 (MQ=255)                                                  gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                              <  1:316514/149‑1 (MQ=255)                                                  gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                              <  3:290450/149‑1 (MQ=255)                                                  gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                              <  7:88707/149‑1 (MQ=255)                                                   cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACTGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                             <  5:35646/149‑1 (MQ=255)                                                    ccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                            >  2:177554/1‑149 (MQ=255)                                                      tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                      >  6:239825/1‑137 (MQ=255)                                                      tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                      <  5:239825/137‑1 (MQ=255)                                                           cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             <  3:347258/125‑1 (MQ=255)                                                           cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             >  4:347258/1‑125 (MQ=255)                                                                 aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGc                                                                                                                       <  8:9991/93‑1 (MQ=255)                                                                 aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGc                                                                                                                       >  7:9991/1‑93 (MQ=255)                                                                 aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                >  5:232370/1‑148 (MQ=255)                                                                     ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                           <  7:256270/149‑1 (MQ=255)                                                                      cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                          >  3:335206/1‑149 (MQ=255)                                                                       gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcc                                                                                >  6:350749/1‑126 (MQ=255)                                                                       gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcc                                                                                <  5:350749/126‑1 (MQ=255)                                                                       gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGc                                                         <  4:298994/149‑1 (MQ=255)                                                                          ccTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAcc                                                      <  6:217946/149‑1 (MQ=255)                                                                            tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                     >  4:308883/1‑116 (MQ=255)                                                                            tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                     <  3:308883/116‑1 (MQ=255)                                                                              cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCATGTCCAGGGTCTGCGAAGTAGCACCCgg                                                   <  1:16996/148‑1 (MQ=255)                                                                              cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                  >  7:138462/1‑149 (MQ=255)                                                                                      ttCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAATTCTGCTTCACAACTTCGACTATGATATCAAGGTCCAGGGTCAGCGAAGTAGCACCCGGGCCTTTGAt                                          >  8:149768/1‑149 (MQ=38)                                                                                                 tACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                 <  2:39124/147‑1 (MQ=255)                                                                                                   cAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                                <  2:365835/146‑1 (MQ=255)                                                                                                      gAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                          >  2:133743/1‑149 (MQ=255)                                                                                                      gAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTATCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                          >  8:205692/1‑149 (MQ=255)                                                                                                      gAGCGGCCTTGTGACGA‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCATAAAATACTTCTGATTCACACCTTCGACACTGATAACCAGGTCCAGGGTCTGCGAAGTAGCACCCGGCCCTTTGAAGCCGAAGACgttcagg                          >  6:2187/1‑149 (MQ=255)                                                                                                                gtgtCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                 <  1:177554/148‑1 (MQ=255)                                                                                                                 tgtCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt               <  1:138562/149‑1 (MQ=255)                                                                                                                   tCGT‑TATAGGGCCGAAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTTCTTCACACCTTCGACTCTGATATCCATGTCCAGGTTCTGCGATGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGTTTCAGCgtggtgg             <  3:109293/149‑1 (MQ=255)                                                                                                                   tCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg             >  8:301525/1‑149 (MQ=255)                                                                                                                   tCGT‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg              <  6:232370/148‑1 (MQ=255)                                                                                                                      t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGTAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg          <  2:43400/149‑1 (MQ=255)                                                                                                                      t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                         >  6:214363/1‑134 (MQ=255)                                                                                                                      t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                         <  5:214363/134‑1 (MQ=255)                                                                                                                      t‑GATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg          >  2:30778/1‑149 (MQ=255)                                                                                                                          tAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt       <  5:160680/149‑1 (MQ=255)                                                                                                                             ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                           <  7:227325/126‑1 (MQ=255)                                                                                                                             ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                           >  8:227325/1‑126 (MQ=255)                                                                                                                             ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg     >  1:78656/1‑148 (MQ=255)                                                                                                                              gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg     >  8:97966/1‑147 (MQ=255)                                                                                                                               ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg  >  7:324860/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                         GCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGT‑GATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG  >  NC_002947/5674617‑5674889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑