Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,013,885	T→C	intergenic (+90/+20)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,885	0	T	C	100.0%	111.8 / NA	35	intergenic (+90/+20)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/19);  total (16/19)

AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCA  >  NC_002947/6013740‑6014025                                                                                                                                                  |                                                                                                                                              aaTCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCCCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGc                                                                                                                                             >  6:95579/1‑148 (MQ=255)        ccctcGGGTGAAGTCGCTGGAAGGTGGGGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGcc                                                                                                                                     <  5:297242/145‑1 (MQ=255)         gCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGcc                                                                                                                                     <  8:71599/148‑1 (MQ=255)                  aaGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCCGTGCAGCACAGGTGTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCCCCACCCgtt                                                                                                                           >  4:103484/1‑149 (MQ=37)                        cTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                                                     >  5:135573/1‑149 (MQ=255)                                  gtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTGTCGAACCCGGTGCCGTACCGGTGGGCGCGGGCGTGCCCGCGAATGGCCGCAACCCATTTTTATGGTTGCCGCCa                                                                                                           >  2:264600/1‑149 (MQ=37)                                     tggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAAt                                                                                                                                         <  5:301215/116‑1 (MQ=255)                                       gtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCGACCCCACGTTCACCACAGGGGAAGCAACCGGTGCCGGACCGGTGGGAGCGGGCGGGCCCCCGAATTGCCCCAACCCATTTATATCGTTTGCCCCCGcccc                                                                                                      >  4:232990/1‑149 (MQ=255)                                            cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCTCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCca                                                                                                  <  4:271535/148‑1 (MQ=255)                                                        aaCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                                     <  1:154666/149‑1 (MQ=255)                                                           gcgACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTa                                                                                  <  7:31551/149‑1 (MQ=255)                                                           gcgACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTa                                                                                  <  3:232990/149‑1 (MQ=255)                                                                        cgcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                                                                       <  6:135573/147‑1 (MQ=255)                                                                         gcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                                                                    <  5:95579/149‑1 (MQ=255)                                                                               aCGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCCACCCGTGTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt                                                              >  7:68238/1‑149 (MQ=38)                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                                          >  3:273058/1‑143 (MQ=255)                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                                          <  4:273058/143‑1 (MQ=255)                                                                                          cacaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                                                                            >  1:164077/1‑124 (MQ=255)                                                                                          cacaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                                                                            <  2:164077/124‑1 (MQ=255)                                                                                            caGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCaa                                                 <  1:264600/149‑1 (MQ=255)                                                                                              ggTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                               <  7:228183/149‑1 (MQ=255)                                                                                                  cagcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑At                                          >  3:52076/1‑149 (MQ=255)                                                                                                     cacaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCg                                       >  2:50382/1‑149 (MQ=255)                                                                                                        aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCAGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGccc                                    >  8:204034/1‑149 (MQ=255)                                                                                                           tctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAg                                 >  2:129286/1‑149 (MQ=255)                                                                                                             tcGAACGCTGTGCCGTACCTGTCGGAGCGGGCGTGCCCGCGAATGGCCCCAACACTTTTATCTCGTTGCCGCCATCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAATAACCTGCGGCTGCACCAACTGC‑ATCATCCCCAgct                               >  4:30946/1‑147 (MQ=255)                                                                                                               gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGt                               <  3:103484/147‑1 (MQ=255)                                                                                                                   gCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCttt                         >  2:224527/1‑149 (MQ=255)                                                                                                                              cgtgtgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCGCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCTTTCATACCCCTc               <  6:186912/146‑1 (MQ=255)                                                                                                                               cTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCTTTCATACCCCTCCg             >  3:56750/1‑149 (MQ=255)                                                                                                                                  tgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCTTTCATACCCCTCCgg            <  4:52076/147‑1 (MQ=255)                                                                                                                                  tgGGAGCGGGCGGGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCATCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCAATCCGCCCCAGGTGGCTTTCATACCCCTCCGGc           <  1:50382/149‑1 (MQ=255)                                                                                                                                         gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGcccc                                   >  4:232068/1‑117 (MQ=255)                                                                                                                                         gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGcccc                                   <  3:232068/117‑1 (MQ=255)                                                                                                                                          ggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCa  <  1:353566/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGC‑ATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCA  >  NC_002947/6013740‑6014025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑