Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	515,173	C→T	G190D (GGT→GAT)	coq ←	2‑nonaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	515,173	0	C	T	100.0%	146.0 / NA	44	G190D (GGT→GAT)	coq	2‑nonaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

AGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAACCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGCTTGCACACCTGATGTTCGGTAGCGGC  >  NC_002947/515028‑515315                                                                                                                                                  |                                                                                                                                               agaATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAatcac                                                                                                                                             >  5:304958/1‑149 (MQ=255)   aaTGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCg                                                                                                                                           >  3:141882/1‑149 (MQ=255)              gCACGGCGCGCGCCAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGt                                                                                                                                  <  2:278814/147‑1 (MQ=255)              gCACGGCGCGCGCCAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTg                                                                                                                                 <  6:304958/148‑1 (MQ=255)                           cAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGc                                                                                                                   >  7:174439/1‑149 (MQ=255)                              gTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGc                                                                                                                       >  3:3883/1‑142 (MQ=255)                              gTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGc                                                                                                                       <  4:3883/142‑1 (MQ=255)                                                aTCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATc                                                                                              >  2:195462/1‑149 (MQ=255)                                                   cGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATc                                                                                              >  8:204632/1‑146 (MQ=255)                                                   cGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATc                                                                                              <  7:204632/146‑1 (MQ=255)                                                        tGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTc                                                                                         >  5:208498/1‑146 (MQ=255)                                                                ccTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGccc                                                                              <  4:141882/149‑1 (MQ=255)                                                                ccTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACAGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGccc                                                                              >  4:169962/1‑149 (MQ=255)                                                                 cTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGTAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCg                                                                             >  5:113931/1‑149 (MQ=255)                                                                 cTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCg                                                                             <  6:208498/149‑1 (MQ=255)                                                                    cAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGa                                                                          >  7:28301/1‑149 (MQ=255)                                                                          cGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTc                                                                    <  8:28301/149‑1 (MQ=255)                                                                                 tgCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCg                                                             >  1:289414/1‑149 (MQ=255)                                                                                  gCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGGGGACGGGGCCCGGGAAGCGTTAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTGATCTTCGAAGCGCATCTGCGGCACTATGGCCCGGGATTTTACAAGTTCg                                                             >  2:56709/1‑148 (MQ=255)                                                                                   ctctTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTg                                                           <  1:195462/149‑1 (MQ=255)                                                                                        ggTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGTAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCgg                                                        <  6:113931/147‑1 (MQ=255)                                                                                         gTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCggg                                                       >  4:151346/1‑147 (MQ=255)                                                                                         gTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCggg                                                       <  3:151346/147‑1 (MQ=255)                                                                                          tCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCg                                                                             <  2:57265/124‑1 (MQ=255)                                                                                          tCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCg                                                                             >  1:57265/1‑124 (MQ=255)                                                                                             tGACCTTGGCCAGCAAGCTCATGCCAAATCGGACGGGGGCCTGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAgctgc                                                 <  7:356718/149‑1 (MQ=255)                                                                                             tGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAgctgc                                                 <  1:347627/149‑1 (MQ=255)                                                                                                 cTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCa                                             <  8:174439/149‑1 (MQ=255)                                                                                                    tgCCAGCAAGCTAATTACAATGCGGACGGGGGGCGGGAAGCGATAATCACCGGCCGCCAGTGCAGAGTCTGCGTGCTGTTCTTCGTCGATGCTCATCTGCTCGATAATGGCCTGGGATTTTTCATCTTCGTGCGGGATCTGCTCCAg                                            <  6:237539/146‑1 (MQ=255)                                                                                                      ccAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGc                                        >  3:195407/1‑149 (MQ=255)                                                                                                       cAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTg                                         >  1:108730/1‑147 (MQ=255)                                                                                                       cAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTg                                         <  2:108730/147‑1 (MQ=255)                                                                                                              cTCATGCCAAAGCGGACGGGGGGCGGGATGCGATAATCACAGGCTTCCAGTGGAGACTCGGCGAGCTGATCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCTGCTTCGTGCGGGAGCTGCTCGAGGTGCTCGTCGAg                                <  1:56709/149‑1 (MQ=255)                                                                                                                 aTGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAgg                               <  4:195407/147‑1 (MQ=255)                                                                                                                 aTGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTg                             >  8:324336/1‑149 (MQ=255)                                                                                                                 aTGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTg                             >  6:293614/1‑149 (MQ=255)                                                                                                                  tGCCAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCGCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGc                            <  2:289414/149‑1 (MQ=255)                                                                                                                     cAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTc                                                                                         <  7:319963/85‑1 (MQ=255)                                                                                                                     cAAAGCGGACGGGGGCCGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTc                                                                                         >  8:319963/1‑85 (MQ=255)                                                                                                                                    ccGGGAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGCTTGCACACCTGATGTTCg          >  3:73127/1‑149 (MQ=255)                                                                                                                                       ggAAGCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGCTTGCACACCTGATGTTCGGTa       <  1:206060/149‑1 (MQ=255)                                                                                                                                           gCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTg                                                           <  4:357091/93‑1 (MQ=255)                                                                                                                                           gCGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTg                                                           >  3:357091/1‑93 (MQ=255)                                                                                                                                            cGATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGCTTGCACACCTGATGTTCGGTAgcggc  >  2:366428/1‑149 (MQ=255)                                                                                                                                             gATAATCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGCTTGCACACCTGATGTTCGGTAgcggc  >  6:254605/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                               AGAATGCCTGCTTGCACGGCGCGCGCCAGGTCCTTGGCGTCGAATCGATCCGTCATGGCGATGCCTTCAGATGCGGTATGTGCTCTTGGTCATGACCTTGGCCAGCAAGCTCATGCCAAAGCGGACGGGGGCCGGGAAGCGATAACCACCGGCTTCCAGTGCAGACTCGGCGTGCTGTTCTTCGTCGATGCGCATCTGCTCGAGAATGGCCCGGGATTTTTCATCTTCGTGCGGGAGCTGCTCCAGGTGCTCGTCGAGGTGCTTGCACACCTGATGTTCGGTAGCGGC  >  NC_002947/515028‑515315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑