Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,499,508 +C intergenic (+85/‑79) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,499,5061.C97.1% 97.6 / ‑4.4 35intergenic (+83/‑81)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base C (12/21);  minor base G (1/0);  total (14/21)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

AGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT  >  NC_002947/1499366‑1499640
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aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑‑cccgctc                                                                                                                                  <  2:40030/149‑6 (MQ=25)
   ccGTGATGCGGCCATTCAGACCCTGAACCACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAACCGGTGGGAGCGGGCGTCCCCGCTAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCGGGT‑GT‑TCGCGGCCACGC‑CC‑GCTCCca                                                                                                                               <  6:306501/149‑1 (MQ=18)
    cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTACCAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCCCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TAGCGGGCACGC‑CC‑GCTCCca                                                                                                                               >  2:20832/1‑148 (MQ=21)
         tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGAc                                                                                                                          <  6:186338/148‑1 (MQ=21)
               cATTCAGAGCCTGAACTAAGCCTTGGCACCACTCCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGc                                                                                                                   <  8:205677/149‑1 (MQ=21)
                    agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCCCCGTGCATGGCACC‑CGCTTTGCCGGT‑GT‑TAGCGGGCACGC‑CC‑GCTCCCCCAGACTGATTGCCAta                                                                                                               >  4:316877/1‑147 (MQ=21)
                       gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGc                                                                                                           <  5:169620/149‑1 (MQ=21)
                            acctaaGCCTTGGCACAACCGCAGCACTGTGGCTCCCAACGGGGGGGCGGGGGGGGCCCCGCTAACCAGGCGCCGCGGT‑CCTGGCCCCGGGCTTGGCGGGT‑GTGC‑GCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                                       <  2:344247/144‑1 (MQ=255)
                            aagcaaGCCTTGCCACCCCAGCCGGACTGCGGCTGCCAACCGGGGGGTCCGGGCGGGCCCGCTAAACTGGGCCCGGGGTGCATGGCACG‑GGTTTTGCCGGT‑GT‑TCGGGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                                       <  2:144009/144‑1 (MQ=255)
                              cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGAtt                                                                                                                     >  5:70945/1‑132 (MQ=18)
                              cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGAtt                                                                                                                     <  6:70945/132‑1 (MQ=18)
                                            cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGAGCCCCGGTGCATGGCACC‑GGGATTGGCCGT‑GT‑TCGCGGGCCCG‑‑CCAGCAGCCCCAGACAGCTTGCCATTAATCGGGCCGATCGCGACCTTGACg                                                                                      >  2:269692/1‑149 (MQ=12)
                                                                          gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                                                        <  4:276694/149‑1 (MQ=25)
                                                                            gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                      <  8:346796/149‑1 (MQ=25)
                                                                            gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                      >  7:37042/1‑149 (MQ=25)
                                                                                  gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                                >  6:164727/1‑149 (MQ=25)
                                                                                  gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                                >  6:335789/1‑149 (MQ=25)
                                                                                    gCCCGCGAAACCGGCGCCGCGGTGCATGCCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                              <  5:294165/149‑1 (MQ=37)
                                                                                    gCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                              <  2:106586/149‑1 (MQ=37)
                                                                                     cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                              <  5:338274/148‑1 (MQ=37)
                                                                                            aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCGCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc                                      <  1:20832/149‑1 (MQ=37)
                                                                                              cAGGCGCCGCGGTTCAGGGCAGC‑GGCTTTGCCGGTGGT‑C‑GCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                    <  7:246569/149‑1 (MQ=38)
                                                                                                 gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                  >  3:162670/1‑148 (MQ=37)
                                                                                                   gccgcGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                               <  3:316877/149‑1 (MQ=37)
                                                                                                    ccgcGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                    >  7:46428/1‑127 (MQ=25)
                                                                                                    ccgcGGTGCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                    <  8:46428/127‑1 (MQ=25)
                                                                                                           gCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                        <  4:283689/100‑1 (MQ=21)
                                                                                                           gCATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                        >  3:283689/1‑100 (MQ=21)
                                                                                                            cATGGCACC‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg                       >  5:24700/1‑148 (MQ=38)
                                                                                                               ggcacc‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTGTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaaa                    >  1:33375/6‑149 (MQ=38)
                                                                                                                gcacc‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTCTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc                   <  2:33375/144‑1 (MQ=38)
                                                                                                                  acc‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg                                                                                 >  2:200542/3‑84 (MQ=21)
                                                                                                                  acc‑GGCTTTGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg                                                                                 <  1:200542/82‑1 (MQ=21)
                                                                                                                         tttGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt                          <  5:97323/133‑1 (MQ=39)
                                                                                                                         tttGCCGGT‑GT‑TCGCGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt                          >  6:97323/1‑133 (MQ=39)
                                                                                                                                   tgttcgcGGGCACGC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt  <  3:83948/143‑1 (MQ=255)
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AGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT  >  NC_002947/1499366‑1499640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑