| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.3% | 114.5 / ‑6.1 | 38 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (17/19); minor base T (0/1); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932494‑1932776 | cggctTCATCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAACGATATGCGTCCCCAGTAGGAGCCGGCTTG‑c‑ccg > 2:194526/1‑147 (MQ=39) ggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCGACAAAGGGAAAGCGTGCCCGGGAGGGGCGGGCTTggccgc > 8:236278/1‑144 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CACGCGAAggg > 5:141635/1‑149 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑Tg > 5:141915/1‑148 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑Tg > 2:275449/1‑148 (MQ=255) cATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGaa < 1:321787/129‑1 (MQ=255) gccAGCGCCATTGCAGGTCGCACGAAAATAGCCGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACATAGCATATGCGTCCCCTGTAGGAGCGGGCTTGTC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTg < 1:228386/146‑1 (MQ=37) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c‑ccgcga < 1:203906/120‑6 (MQ=255) gcaaTTGCTGGTCTCACTAAAATTGCAGAAATCGCTGGGGCGGCTTTGGGGCCCTTGGCGGGCAAGCCGGCTCCTAAAAAGGAATTGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTa < 6:140262/146‑1 (MQ=25) gTCTCCCGAAAATCGCTGTACACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCCCCACCAACGGATATGCGTCCCCTGTAGGAGGGGGCGTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 2:257159/149‑1 (MQ=37) tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCTAAAAAGGATATGCGTCCCCAGGAGGAGCGGGCGTGCC‑CGCGAATGGC‑AGCAAGGCACCCCCAAATTGTCGACAACGCGGAtactc > 7:196037/1‑145 (MQ=37) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c < 1:9772/99‑1 (MQ=255) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c > 2:9772/1‑99 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCCGCCCCAAATTGTCGGCTACGCGGAGCAGCgctgcg > 8:84387/1‑144 (MQ=37) aaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCGCTGATCCgcg < 7:263849/149‑3 (MQ=37) cGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa < 4:56458/149‑1 (MQ=255) gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg > 8:286754/1‑149 (MQ=255) gggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 1:275449/149‑1 (MQ=255) ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CCCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTAGACAACGCCGATCAGCGCTCCCGCAGGGTGATGTTCAGCTc > 2:221937/1‑147 (MQ=37) ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCGGTAGGAGCGGGCTTGCC‑CGCGCAGGGC‑AGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGGAGGGGGATGTTGAGcgg > 6:316233/1‑145 (MQ=37) gCGGCCCGTCGCGGGCAAGCCCGCTCATACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag < 1:148645/148‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa > 7:57377/1‑126 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 3:336752/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCATGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 1:102467/1‑147 (MQ=255) cGCTCCTACAATGGATATGCGTCCCCTTTAGGAGCGGGGTTGCC‑CGCGAAGGTC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 5:137722/149‑1 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTTTCGTAGCGGGTTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGTCATGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 1:194526/149‑1 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATACGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTcca > 3:333051/1‑147 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 8:87169/1‑149 (MQ=255) cAACGGATATGCGCCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat < 5:316233/147‑1 (MQ=255) aaaaGGATATGCGGCCCCTGTTGTCGGGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg < 8:196037/147‑1 (MQ=255) ggATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 1:151238/1‑148 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc > 2:97719/1‑149 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt > 7:188756/1‑119 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt < 8:188756/119‑1 (MQ=255) tCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc < 6:104667/141‑1 (MQ=255) tCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc > 5:104667/1‑141 (MQ=255) ccTGTAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa < 7:84387/149‑1 (MQ=255) tAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCTGTCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 7:243051/149‑1 (MQ=255) tAGGAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt > 8:161663/1‑149 (MQ=255) aggaggGGGCTGGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 1:221937/148‑1 (MQ=255) ggAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCTAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 1:97719/148‑1 (MQ=255) ggAGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 7:161663/148‑1 (MQ=255) aGCGGGCTTGCC‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca > 3:340218/1‑149 (MQ=255) | CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑CGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932494‑1932776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |