Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,586,035	+G	intergenic (+118/+97)	PP_4061 → / ← PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,586,033	1	.	G	100.0%	55.8 / NA	18	intergenic (+116/+99)	PP_4061/PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (6/12);  total (6/12)

GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCG  >  NC_002947/4585887‑4586117                                                                                                                                                    |                                                                                       gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg                                                                                      <  5:286670/149‑1 (MQ=255)     aGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggta                                                                                   >  2:338348/1‑145 (MQ=255)              ccACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc                                                                          <  1:335052/149‑1 (MQ=255)              ccACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTAGCGGGTAAAcccccac                                                                           >  4:122463/1‑144 (MQ=255)                aCCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTa                                                                        >  4:343942/1‑149 (MQ=255)                  cGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAcc                                                                      <  6:83299/149‑1 (MQ=255)                    gTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑g                                                                   >  7:265529/1‑149 (MQ=255)                       aaGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGa                                                                <  4:347258/149‑1 (MQ=255)                              aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGA‑ccgtgc                                                         <  1:338348/148‑6 (MQ=39)                                 ggTAGACCACGGCACCGGCGTGAACAGCTGAGCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGGGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGc                                                      <  6:176559/149‑1 (MQ=38)                                  gTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGcc                                                     <  4:287350/149‑1 (MQ=39)                                  gTAGACCACGGAACCGGCGTGAAGAGCTGATCTGCGCTGCTCCATTGAAAGGTTGCTGAGGACCGGGTGCTTGTCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑GGGACCGTGCCGGcc                                                     <  6:237590/149‑1 (MQ=25)                                           ggCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATgcg                                            <  8:86046/149‑1 (MQ=39)                                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                             <  2:71064/149‑1 (MQ=39)                                                                    cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                      <  8:265529/146‑1 (MQ=38)                                                                         ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt                              >  7:334170/1‑133 (MQ=38)                                                                         ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt                              <  8:334170/133‑1 (MQ=38)                                                                                     gTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTTAACCCGCTCCTACCAGGGGC‑CGTGGCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGGTTTCCCGCGAAGAggccg  >  5:287493/1‑149 (MQ=37)                                                                                                            cAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg                     >  7:211847/1‑107 (MQ=25)                                                                                                            cAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg                     <  8:211847/107‑1 (MQ=25)                                                                                                                                                    |                                                                                       GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCG  >  NC_002947/4585887‑4586117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑