Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,751	+G	intergenic (+113/+27)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,750	1	.	G	100.0%	242.2 / NA	68	intergenic (+112/+28)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (35/33);  total (35/33)

GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674603‑5674888                                                                                                                                                     |                                                                                                                                            ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑g                                                                                                                                            <  5:267418/149‑1 (MQ=255) ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑g                                                                                                                                            <  4:317439/149‑1 (MQ=255)  gTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑gg                                                                                                                                           <  8:221996/149‑2 (MQ=255)          ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCg                                                                                                                                    >  1:344724/1‑149 (MQ=255)                 tgctgcCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTGCTGCAGAGACTTTGTTGATAGGTACAGGAGGGGCCTTGTGTCGTGATATGGCCGCAAAGCGGCCCCCTCGGgttt                                                                                                                               >  6:161377/1‑147 (MQ=25)                        ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCt                                                                                                                         <  5:237905/145‑1 (MQ=255)                        ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCt                                                                                                                         >  6:237905/1‑145 (MQ=255)                        ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTgg                                                                                                                       >  5:26272/1‑147 (MQ=255)                                  tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACt                                                                                                            >  1:131741/1‑148 (MQ=255)                                    aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACtt                                                                                                           <  7:13551/147‑1 (MQ=255)                                    aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTc                                                                                                          <  7:116487/148‑1 (MQ=255)                                     tAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTg                                                                                                        <  6:371042/149‑1 (MQ=255)                                         aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAGTAATTTCTGcca                                                                                                     <  7:1124/148‑3 (MQ=37)                                          gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTca                                                                                                   <  2:85281/149‑1 (MQ=255)                                              cGAGGGGGCTGCTTCGCAGCCCTATCACGACACCAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                               <  2:75411/149‑1 (MQ=255)                                               gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCt                                                                                              >  3:39630/1‑149 (MQ=255)                                                 gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                            <  6:26272/149‑1 (MQ=255)                                                  ggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                           <  6:83812/149‑1 (MQ=255)                                                   gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          <  3:185170/149‑1 (MQ=255)                                                       tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGGAATTACTTCTGCTTCACACCTTCGACACt                                                                                      >  5:328141/1‑149 (MQ=255)                                                        gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                     >  7:185143/1‑149 (MQ=255)                                                            cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                 >  1:159654/1‑149 (MQ=255)                                                             gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                >  4:237189/1‑149 (MQ=255)                                                                  ccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                           >  8:56364/1‑149 (MQ=255)                                                                     aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtc                                                                          >  4:10163/1‑147 (MQ=255)                                                                        aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  7:329272/148‑1 (MQ=255)                                                                        aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  8:185143/148‑1 (MQ=255)                                                                        aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                     >  7:198271/1‑149 (MQ=255)                                                                          gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACCGATATCCAGGTCCAGGGTc                                                                   >  4:225441/1‑149 (MQ=255)                                                                             acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                >  5:30041/1‑149 (MQ=255)                                                                               aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                             >  4:149779/1‑134 (MQ=255)                                                                               aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                             <  3:149779/134‑1 (MQ=255)                                                                                aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                             <  2:159654/149‑1 (MQ=255)                                                                                 ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                            <  3:237189/149‑1 (MQ=255)                                                                                   ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCTCACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                <  2:207671/143‑1 (MQ=255)                                                                                   ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCTCACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                >  1:207671/1‑143 (MQ=255)                                                                                         cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGAAccc                                                    >  5:101907/1‑149 (MQ=255)                                                                                          tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                   >  6:2204/1‑149 (MQ=255)                                                                                             agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGc                                                >  5:172725/1‑149 (MQ=255)                                                                                             agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGc                                                >  3:33304/1‑149 (MQ=255)                                                                                             agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACCGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGc                                                <  3:225441/149‑1 (MQ=255)                                                                                              gagaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGcc                                               >  6:229419/1‑149 (MQ=255)                                                                                                gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                             <  1:226158/149‑1 (MQ=255)                                                                                                  aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTg                                           <  4:39630/149‑1 (MQ=255)                                                                                                    aagaaattctcTGCAGGAGCGGCCTTGTGTCGTGATCGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACAGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCCGGCCTTTGAt                                         >  6:115952/8‑149 (MQ=37)                                                                                                         gctctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCCCACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                     <  8:288472/146‑1 (MQ=255)                                                                                                         gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                     <  5:175241/148‑1 (MQ=255)                                                                                                            ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  3:322302/1‑149 (MQ=255)                                                                                                            ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  6:54444/1‑149 (MQ=255)                                                                                                             tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                            >  5:124260/1‑137 (MQ=255)                                                                                                             tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                            <  6:124260/137‑1 (MQ=255)                                                                                                             tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                >  4:186099/1‑149 (MQ=255)                                                                                                              ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                               >  1:59952/1‑149 (MQ=255)                                                                                                               tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgt                              >  5:312255/1‑149 (MQ=255)                                                                                                                 cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                               <  3:186099/146‑1 (MQ=255)                                                                                                                           cTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATACAGGTCCAGGGTCTGCGATGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTAAGCg                  >  7:132574/1‑149 (MQ=255)                                                                                                                               tgtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg               <  5:2204/148‑1 (MQ=255)                                                                                                                               tgtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg               <  2:305600/148‑1 (MQ=255)                                                                                                                                gtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      >  6:174733/1‑92 (MQ=255)                                                                                                                                gtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  5:174733/92‑1 (MQ=255)                                                                                                                                   gTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          <  6:30041/149‑1 (MQ=255)                                                                                                                                   gTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          >  2:308344/1‑149 (MQ=255)                                                                                                                                    tGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTGCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          >  1:232476/1‑149 (MQ=255)                                                                                                                                        aGGGCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt     >  2:132094/1‑149 (MQ=255)                                                                                                                                          ggCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  5:183685/117‑1 (MQ=255)                                                                                                                                          ggCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   >  6:183685/1‑117 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                         <  4:336346/110‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  4:130503/149‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  1:145920/149‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  4:33304/149‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  5:229419/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTT‑CAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674603‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑