Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,264,120	C→T	P24S (CCT→TCT)	accD →	acetyl‑CoA carboxylase carboxyltransferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,264,120	0	C	T	100.0%	162.3 / NA	48	P24S (CCT→TCT)	accD	acetyl‑CoA carboxylase carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

ATAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGCCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGC  >  NC_002947/2263974‑2264262                                                                                                                                                   |                                                                                                                                               aTAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCt                                                                                                                                              <  5:277905/149‑1 (MQ=255)    aaaGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGa                                                                                                                                            <  3:69400/148‑1 (MQ=255)    aaaGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAg                                                                                                                                           >  5:263073/1‑149 (MQ=255)       gATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGc                                                                                                                                        <  6:263073/149‑1 (MQ=255)              aGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTgg                                                                                                                                  >  1:259055/1‑148 (MQ=255)                cTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGca                                                                                                                                >  5:281714/1‑148 (MQ=255)                     gggcagggcagggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTg                                                                                                                          >  7:150933/1‑149 (MQ=255)                         agggcagggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCg                                                                                                                      <  5:75887/149‑1 (MQ=255)                             cagggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCt                                                                                                                  <  5:140772/149‑1 (MQ=255)                               gggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGGGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGc                                                                                                                <  6:302397/149‑1 (MQ=255)                                 gcCGGTTACGCCGGCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGa                                                                                                              <  3:141550/149‑1 (MQ=255)                                      tGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCg                                                                                                         >  4:215196/1‑149 (MQ=255)                                          gCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCt                                                                                                     <  7:260660/149‑1 (MQ=255)                                            cGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTg                                                                                                    >  6:229952/1‑148 (MQ=255)                                             gTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTa                                                                                                  <  6:328613/149‑1 (MQ=255)                                             gTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTa                                                                                                  <  2:259055/149‑1 (MQ=255)                                                 cccGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATctt                                                                                              >  7:270314/1‑147 (MQ=255)                                                 cccGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGATGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGt                                                                                              <  2:261908/149‑1 (MQ=255)                                                      cTGCCAATACTGGATAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGa                                                                                         <  6:350771/149‑1 (MQ=255)                                                           aaTACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTgg                                                                                    <  7:156912/149‑1 (MQ=255)                                                           aaTACTGGAGAAAGAAAGCATGAGCAACGGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTgg                                                                                    <  8:150933/149‑1 (MQ=255)                                                                tGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaa                                                                                  >  4:3516/1‑146 (MQ=255)                                                                tGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaa                                                                                  >  3:168049/1‑146 (MQ=255)                                                                tGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaa                                                                                  >  2:44481/1‑146 (MQ=255)                                                                  gagaAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAgg                                                                                                                                          >  3:258576/1‑88 (MQ=255)                                                                  gagaAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAgg                                                                                                                                          <  4:258576/88‑1 (MQ=255)                                                                  gagaAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGAc                                                                             >  6:46105/1‑149 (MQ=255)                                                                  gagaAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGAc                                                                             >  4:17381/1‑149 (MQ=255)                                                                    gaaagaaaGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGAccc                                                                           <  6:281714/149‑1 (MQ=255)                                                                       agaaagCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTg                                                                         >  6:333473/1‑148 (MQ=255)                                                                         aaagCATGAGAAACTGGTTAGTCGACAAACTGATCCCTTAGATCATGCGTTCCGAGGTGATAAAGAGATCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCTTCCTGCGAGGCCGTGCTGTATCGTCCGGAGATGGAAAAGCCCCTGGAt                                                                      >  8:176478/1‑149 (MQ=255)                                                                                         gTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGc                                                       >  8:184968/1‑148 (MQ=255)                                                                                                gACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATcacca                                               <  4:168049/149‑1 (MQ=255)                                                                                                     aCTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATgc                                          <  8:270314/149‑1 (MQ=255)                                                                                                     aCTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATgc                                          <  5:333473/149‑1 (MQ=255)                                                                                                             cTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGgc                                  >  1:285612/1‑149 (MQ=255)                                                                                                                cGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGgcgc                                <  3:17381/148‑1 (MQ=255)                                                                                                                  aTCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACg                             >  1:330657/1‑149 (MQ=255)                                                                                                                        cGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTgcgc                        <  3:215196/148‑1 (MQ=255)                                                                                                                        cGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTgcgc                        <  2:156334/148‑1 (MQ=255)                                                                                                                               aGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGAc                <  1:44481/149‑1 (MQ=255)                                                                                                                                   gAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCt            >  7:291146/1‑149 (MQ=255)                                                                                                                                   gAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCt            >  1:290780/1‑149 (MQ=255)                                                                                                                                    aaaaaGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCtt           >  7:267724/1‑149 (MQ=255)                                                                                                                                     aaaaGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTc          >  3:97660/1‑149 (MQ=255)                                                                                                                                       aaGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCt        <  4:97660/149‑1 (MQ=255)                                                                                                                                       aaGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGGCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCt        <  7:184968/149‑1 (MQ=255)                                                                                                                                             tCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGc  >  6:326662/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                               ATAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGCCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGC  >  NC_002947/2263974‑2264262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑